Restriction map for
pFN21AE5352
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI Bsp1286I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 21 GGTACC G^GTACC
AhdI 896 GACGAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 802 GATCC GGATC(4/5)
Asp700I 446 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 21 GGTACC G^GTACC
AspEI 896 GACGAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 21 GGTACC G^G[CT][GA]CC
BbsI 334 GAAGAC GAAGAC(2/6)
BpmI 31 CTGGAG CTGGAG(16/14)
BpuAI 334 GAAGAC GAAGAC(2/6)
BsgI 824 CTGCAC GTGCAG(16/14)
BsmFI 881 GGGAC GGGAC(10/14)
BsmI 766 GAATGC GAATGC(1/-1)
Bsp1407I 118 TGTACA T^GTACA
BsrGI 118 TGTACA T^GTACA
BstAPI 51 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 443 TTCGAA TT^CGAA
BstNI 457 CCAGG CC^[AT]GG
BstYI 801 AGATCC [GA]^GATC[CT]
DpnII 802 GATC ^GATC
DraI 567 TTTAAA TTT^AAA
DrdI 247 GACCAGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 896 GACGAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 457 CCAGG ^CC[AT]GG
EcoRV 358 GATATC GAT^ATC
EcoT14I 635 CCAAGG C^C[AT][AT]GG
KpnI 21 GGTACC GGTAC^C
MboI 802 GATC ^GATC
MflI 801 AGATCC [GA]^GATC[CT]
MspA1I 266 CAGCTG C[AC]G^C[GT]G
MvaI 457 CCAGG CC^[AT]GG
NdeII 802 GATC ^GATC
NspV 443 TTCGAA TT^CGAA
PaeR7I 545 CTCGAG C^TCGAG
PleI 474 GACTC GAGTC(4/5)
PstI 188 CTGCAG CTGCA^G
PvuII 266 CAGCTG CAG^CTG
Sau3AI 802 GATC ^GATC
ScaI 588 AGTACT AGT^ACT
SfuI 443 TTCGAA TT^CGAA
SmlI 545 CTCGAG C^T[CT][GA]AG
SspBI 118 TGTACA T^GTACA
StyI 635 CCAAGG C^C[AT][AT]GG
TfiI 177 GATTC G^A[AT]TC
XhoI 545 CTCGAG C^TCGAG
XhoII 801 AGATCC [GA]^GATC[CT]
XmnI 446 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AvaI 44 CTCGGG C^[CT]CG[GA]G
545 CTCGAG
AvaII 255 GGTCC G^G[AT]CC
903 GGTCC
BfaI 341 CTAG C^TAG
641 CTAG
BsaJI 25 CCCCGG C^C[ACGT][ACGT]GG
635 CCAAGG
BsoBI 44 CTCGGG C^[CT]CG[GA]G
545 CTCGAG
BstF5I 303 GGATG GGATG(2/0)
348 GGATG
DdeI 145 CTAAG C^T[ACGT]AG
941 CTGAG
EaeI 2 TGGCCG [CT]^GGCC[GA]
857 TGGCCG
EcoRI 286 GAATTC G^AATTC
406 GAATTC
FokI 303 GGATG GGATG(9/13)
348 GGATG
HinfI 177 GATTC G^A[ACGT]TC
474 GACTC
HphI 670 GGTGA GGTGA(8/7)
454 TCACC
MaeI 341 CTAG C^TAG
641 CTAG
MslI 478 CATTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
761 CATCTGAATG
SfaNI 550 GATGC GCATC(5/9)
814 GCATC
TaqI 444 TCGA T^CGA
546 TCGA
Tsp45I 324 GTCAC ^GT[GC]AC
511 GTGAC
TthHB8I 444 TCGA T^CGA
546 TCGA
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
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AcsI AfaI AluI ApoI BbvI
BcnI BsiYI BslI BsrI BssKI
Cfr13I EarI Fnu4HI HaeIII HapII
HpaII ItaI Ksp632I MaeIII MboII
MnlI MseI MspI MwoI NciI
NlaIII NlaIV RsaI Sau96I ScrFI
SfcI Tru9I TseI Tsp509I TspRI