Restriction map for
pFN21AE6565
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# NO CUT ENZYMES ( 195 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseAI BsiEI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MaeII MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AciI 28 GCGG CCGC(-3/-1)
AlwI 489 GATCC GGATC(4/5)
AseI 448 ATTAAT AT^TAAT
AsnI 448 ATTAAT AT^TAAT
AvaII 442 GGACC G^G[AT]CC
BanII 212 GAGCTC G[GA]GC[CT]^C
BciVI 41 GTATCC GTATCC(6/5)
BpmI 163 CTGGAG CTGGAG(16/14)
BseRI 209 GAGGAG GAGGAG(10/8)
BsgI 245 GTGCAG GTGCAG(16/14)
BsmAI 481 GAGAC GTCTC(1/5)
BsmFI 441 GGGAC GGGAC(10/14)
BsrDI 226 GCAATG GCAATG(2/0)
BstF5I 415 GGATG GGATG(2/0)
DraI 51 TTTAAA TTT^AAA
DraII 441 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 95 CGGCCA [CT]^GGCC[GA]
EcoO109I 441 GGGACCC [GA]G^G[ACGT]CC[CT]
FokI 415 GGATG GGATG(9/13)
HphI 202 GGTGA GGTGA(8/7)
NlaIII 73 CATG CATG^
PpuMI 441 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 448 ATTAAT AT^TAAT
SacI 212 GAGCTC GAGCT^C
SapI 264 GAAGAGC GCTCTTC(1/4)
SspI 451 AATATT AAT^ATT
TfiI 510 GAATC G^A[AT]TC
TspRI 432 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspHI 10 GTGCTC G[AT]GC[AT]^C
212 GAGCTC
BbvI 465 GCAGC GCAGC(8/12)
334 GCTGC
BmyI 10 GTGCTC G[AGT]GC[ACT]^C
212 GAGCTC
BsiHKAI 10 GTGCTC G[AT]GC[AT]^C
212 GAGCTC
Bsp1286I 10 GTGCTC G[AGT]GC[ACT]^C
212 GAGCTC
BssKI 45 CCTGG ^CC[ACGT]GG
492 CCTGG
BstNI 45 CCTGG CC^[AT]GG
492 CCTGG
Cfr13I 3 GGCCC G^G[ACGT]CC
442 GGACC
DpnII 400 GATC ^GATC
489 GATC
EarI 264 GAAGAG CTCTTC(1/4)
436 GAAGAG
EcoRII 45 CCTGG ^CC[AT]GG
492 CCTGG
Fnu4HI 334 GCTGC GC^[ACGT]GC
465 GCAGC
HaeIII 3 GGCC GG^CC
96 GGCC
ItaI 334 GCTGC GC^[ACGT]GC
465 GCAGC
Ksp632I 264 GAAGAG CTCTTC(1/4)
436 GAAGAG
MboI 400 GATC ^GATC
489 GATC
MboII 264 GAAGA GAAGA(8/7)
436 GAAGA
MvaI 45 CCTGG CC^[AT]GG
492 CCTGG
NdeII 400 GATC ^GATC
489 GATC
PleI 22 GACTC GAGTC(4/5)
195 GACTC
Sau3AI 400 GATC ^GATC
489 GATC
Sau96I 3 GGCCC G^G[ACGT]CC
442 GGACC
ScrFI 45 CCTGG CC^[ACGT]GG
492 CCTGG
TaqI 35 TCGA T^CGA
60 TCGA
TseI 334 GCTGC G^C[AT]GC
465 GCAGC
TthHB8I 35 TCGA T^CGA
60 TCGA
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AluI DdeI HinfI MnlI MseI
NlaIV SfaNI Tru9I Tsp509I