##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstF5I      BstNI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRI       EcoRII      EcoRV       EcoT22I     FauI        
FbaI        Fnu4HI      FokI        FseI        FspI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
ItaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TseI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AcsI          384  AAATTC               [GA]^AATT[CT]
AfaI          161  GTAC                 GT^AC
AflII         645  CTTAAG               C^TTAAG
ApoI          384  AAATTC               [GA]^AATT[CT]
AseI           58  ATTAAT               AT^TAAT
AsnI           58  ATTAAT               AT^TAAT
AvaII          77  GGTCC                G^G[AT]CC
BfaI          277  CTAG                 C^TAG
BfrI          645  CTTAAG               C^TTAAG
BsaI          187  GGTCTC               GGTCTC(1/5)
BsaJI         170  CCATGG               C^C[ACGT][ACGT]GG
BsmAI         188  GTCTC                GTCTC(1/5)
BstEII        606  GGTTACC              G^GT[ACGT]ACC
BstPI         606  GGTTACC              G^GT[ACGT]ACC
Cfr13I         77  GGTCC                G^G[ACGT]CC
DsaI          170  CCATGG               C^C[GA][CT]GG
EcoO65I       606  GGTTACC              G^GT[ACGT]ACC
EcoT14I       170  CCATGG               C^C[AT][AT]GG
HindIII       465  AAGCTT               A^AGCTT
HinfI         262  GATTC                G^A[ACGT]TC
HphI          325  GGTGA                GGTGA(8/7)
MaeI          277  CTAG                 C^TAG
MfeI            7  CAATTG               C^AATTG
MnlI           64  GAGG                 CCTC(7/6)
MunI            7  CAATTG               C^AATTG
NcoI          170  CCATGG               C^CATGG
NlaIII        171  CATG                 CATG^
PshBI          58  ATTAAT               AT^TAAT
RsaI          161  GTAC                 GT^AC
Sau96I         77  GGTCC                G^G[ACGT]CC
SfaNI         376  GCATC                GCATC(5/9)
SfcI           46  CTACAG               C^T[GA][CT]AG
StyI          170  CCATGG               C^C[AT][AT]GG
TfiI          262  GATTC                G^A[AT]TC
TspRI         500  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AccI           44  GTCTAC               GT^[AC][GT]AC
              151  GTAGAC              
AluI          466  AGCT                 AG^CT
              681  AGCT                
AlwNI         260  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              374  CAGCATCTG           
BbsI          411  GAAGAC               GAAGAC(2/6)
              495  GAAGAC              
BpuAI         411  GAAGAC               GAAGAC(2/6)
              495  GAAGAC              
TaqI          351  TCGA                 T^CGA
              440  TCGA                
TthHB8I       351  TCGA                 T^CGA
              440  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

DdeI        DpnII       HgaI        MaeIII      MboI        
MboII       MseI        NdeII       Sau3AI      SmlI        
Tru9I       Tsp45I      Tsp509I