Restriction map for
pFN21AE5765
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AvrII BamHI BbeI BbrPI BbvCI
BciVI BcnI BfrI BglI BlnI
BsaHI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FauI
FseI HaeII Hin1I KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflMI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
Sse8387I SspBI StuI SwaI Van91I
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 71 enzymes) #
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Acc65I 711 GGTACC G^GTACC
AflIII 920 ACATGT A^C[GA][CT]GT
AgeI 500 ACCGGT A^CCGGT
AlwNI 2735 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1981 CCTCAGG CC^T[ACGT]AGG
Asp718I 711 GGTACC G^GTACC
AspHI 2762 GAGCTC G[AT]GC[AT]^C
AspI 2318 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 61 CTCGAG C^[CT]CG[GA]G
AviII 2658 TGCGCA TGC^GCA
BalI 729 TGGCCA TGG^CCA
BcgI 53 GCAAGACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 48 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 48 GCTCAGC GC^T[ACGT]AGC
BsaAI 850 TACGTA [CT]AC^GT[GA]
BsaI 497 GAGACC GGTCTC(1/5)
BsaWI 500 ACCGGT [AT]^CCGG[AT]
BsiHKAI 2762 GAGCTC G[AT]GC[AT]^C
BsmI 1229 GCATTC GAATGC(1/-1)
BsoBI 61 CTCGAG C^[CT]CG[GA]G
BspHI 822 TCATGA T^CATGA
BspLU11I 920 ACATGT A^CATGT
BspMI 1947 ACCTGC ACCTGC(4/8)
BsrBI 1912 GAGCGG CCGCTC(-3/-3)
BsrDI 1106 CATTGC GCAATG(2/0)
BsrFI 500 ACCGGT [GA]^CCGG[CT]
Bst1107I 2439 GTATAC GTA^TAC
BstXI 2597 CCAGGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2439 GTATAC GTA^TAC
Bsu36I 1981 CCTCAGG CC^T[ACGT]AGG
CelII 48 GCTCAGC GC^T[ACGT]AGC
CfoI 2659 GCGC GCG^C
Cfr10I 500 ACCGGT [GA]^CCGG[CT]
DraII 1081 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1348 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1981 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1081 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 2501 GAATTC G^AATTC
EcoT14I 1673 CCTTGG C^C[AT][AT]GG
FspI 2658 TGCGCA TGC^GCA
HapII 501 CCGG C^CGG
HgaI 2318 GACGC GACGC(5/10)
HhaI 2659 GCGC GCG^C
HinP1I 2659 GCGC G^CGC
HincII 1678 GTTAAC GT[CT]^[GA]AC
HindII 1678 GTTAAC GT[CT]^[GA]AC
HindIII 1597 AAGCTT A^AGCTT
HpaI 1678 GTTAAC GTT^AAC
HpaII 501 CCGG C^CGG
KpnI 711 GGTACC GGTAC^C
MaeII 851 ACGT A^CGT
MluNI 729 TGGCCA TGG^CCA
MscI 729 TGGCCA TGG^CCA
MslI 937 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 501 CCGG C^CGG
NdeI 1280 CATATG CA^TATG
PaeR7I 61 CTCGAG C^TCGAG
PflFI 2318 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 500 ACCGGT A^CCGGT
PshAI 919 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 822 TCATGA T^CATGA
SacI 2762 GAGCTC GAGCT^C
SapI 340 GAAGAGC GCTCTTC(1/4)
ScaI 1936 AGTACT AGT^ACT
SnaBI 850 TACGTA TAC^GTA
SphI 1356 GCATGC GCATG^C
StyI 1673 CCTTGG C^C[AT][AT]GG
Tsp45I 2419 GTCAC ^GT[GC]AC
Tth111I 2318 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 361 TCTAGA T^CTAGA
XhoI 61 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AccI 1992 GTCTAC GT^[AC][GT]AC
2439 GTATAC
BanI 711 GGTACC G^G[CT][GA]CC
1714 GGTGCC
BanII 64 GAGCCC G[GA]GC[CT]^C
2762 GAGCTC
BglII 2640 AGATCT A^GATCT
2781 AGATCT
BsgI 2045 CTGCAC GTGCAG(16/14)
2522 GTGCAG
BsmFI 584 GGGAC GGGAC(10/14)
2594 GTCCC
Cac8I 1356 GCATGC GC[ACGT]^[ACGT]GC
2403 GCAGGC
EaeI 729 TGGCCA [CT]^GGCC[GA]
2532 TGGCCG
EcoNI 1126 CCTGTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1977 CCTTCCTCAGG
NspI 920 ACATGT [GA]CATG^[CT]
1356 GCATGC
SmlI 37 CTCAAG C^T[CT][GA]AG
61 CTCGAG
SspI 2275 AATATT AAT^ATT
2815 AATATT
TspRI 2018 CAGTG CA[GC]TG[ACGT][ACGT]^
2421 CACTG
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaBI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EarI EcoRII FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TthHB8I XhoII
XmnI