Restriction map for
pFN21AE6765
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbvCI BcgI BclI BfrI
BlnI BsaBI BsiEI BsiWI BsmBI
BsmI BspDI BspLU11I BssHII BstAPI
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MunI
NdeI NheI NotI NruI NspI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 611 GACGTC GACGT^C
AflIII 266 ACGTGT A^C[GA][CT]GT
AocI 444 CCTGAGG CC^T[ACGT]AGG
Asp700I 2091 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1670 TGGCCA TGG^CCA
BbeI 1997 GGCGCC GGCGC^C
Bsp1407I 1925 TGTACA T^GTACA
BsrBI 483 CCGCTC CCGCTC(-3/-3)
BsrGI 1925 TGTACA T^GTACA
Bst1107I 2185 GTATAC GTA^TAC
BstBI 2804 TTCGAA TT^CGAA
BstEII 2217 GGTGACC G^GT[ACGT]ACC
BstPI 2217 GGTGACC G^GT[ACGT]ACC
BstXI 1107 CCAGATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2185 GTATAC GTA^TAC
Bsu36I 444 CCTGAGG CC^T[ACGT]AGG
DrdI 611 GACGTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 444 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2217 GGTGACC G^GT[ACGT]ACC
EcoRI 2010 GAATTC G^AATTC
EcoRV 1309 GATATC GAT^ATC
EcoT22I 1249 ATGCAT ATGCA^T
HgaI 718 GCGTC GACGC(5/10)
HincII 2263 GTCAAC GT[CT]^[GA]AC
HindII 2263 GTCAAC GT[CT]^[GA]AC
KasI 1997 GGCGCC G^GCGCC
MluNI 1670 TGGCCA TGG^CCA
MscI 1670 TGGCCA TGG^CCA
MseI 1937 TTAA T^TAA
NarI 1997 GGCGCC GG^CGCC
NsiI 1249 ATGCAT ATGCA^T
NspV 2804 TTCGAA TT^CGAA
PflMI 2451 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 2628 GCTCTTC GCTCTTC(1/4)
ScaI 1790 AGTACT AGT^ACT
SfoI 1997 GGCGCC GGC^GCC
SfuI 2804 TTCGAA TT^CGAA
SmlI 2410 CTCAAG C^T[CT][GA]AG
SspBI 1925 TGTACA T^GTACA
Tru9I 1937 TTAA T^TAA
Van91I 2451 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 722 CCAACGTGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2091 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 560 GTCTAC GT^[AC][GT]AC
2185 GTATAC
AccIII 66 TCCGGA T^CCGGA
1034 TCCGGA
AcyI 611 GACGTC G[GA]^CG[CT]C
1997 GGCGCC
AlwNI 86 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2452 CAGTTTCTG
ApaI 1552 GGGCCC GGGCC^C
2915 GGGCCC
AvaI 555 CCCGAG C^[CT]CG[GA]G
1518 CTCGGG
BamHI 2693 GGATCC G^GATCC
2741 GGATCC
BbrPI 265 CACGTG CAC^GTG
2677 CACGTG
BglI 1894 GCCCCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2127 GCCAACGCGGC
BglII 960 AGATCT A^GATCT
3031 AGATCT
BlpI 91 GCTGAGC GC^T[ACGT]AGC
1965 GCTGAGC
Bpu1102I 91 GCTGAGC GC^T[ACGT]AGC
1965 GCTGAGC
BsaHI 611 GACGTC G[GA]^CG[CT]C
1997 GGCGCC
BseAI 66 TCCGGA T^CCGGA
1034 TCCGGA
BsgI 283 CTGCAC GTGCAG(16/14)
2713 GTGCAG
BsoBI 555 CCCGAG C^[CT]CG[GA]G
1518 CTCGGG
BspEI 66 TCCGGA T^CCGGA
1034 TCCGGA
BspHI 689 TCATGA T^CATGA
2780 TCATGA
BspMI 307 ACCTGC ACCTGC(4/8)
581 ACCTGC
BssSI 2920 CACGAG CACGAG(-5/-1)
730 CTCGTG
CelII 91 GCTGAGC GC^T[ACGT]AGC
1965 GCTGAGC
HaeII 1997 GGCGCC [GA]GCGC^[CT]
2037 GGCGCT
Hin1I 611 GACGTC G[GA]^CG[CT]C
1997 GGCGCC
MroI 66 TCCGGA T^CCGGA
1034 TCCGGA
NaeI 1207 GCCGGC GCC^GGC
2296 GCCGGC
NcoI 1490 CCATGG C^CATGG
2975 CCATGG
NgoMIV 1207 GCCGGC G^CCGGC
2296 GCCGGC
PmaCI 265 CACGTG CAC^GTG
2677 CACGTG
PmlI 265 CACGTG CAC^GTG
2677 CACGTG
PstI 166 CTGCAG CTGCA^G
313 CTGCAG
PvuII 86 CAGCTG CAG^CTG
1646 CAGCTG
RcaI 689 TCATGA T^CATGA
2780 TCATGA
SfcI 166 CTGCAG C^T[GA][CT]AG
313 CTGCAG
TfiI 2510 GAATC G^A[AT]TC
2970 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII