Restriction map for
pFN21AE2714
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# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcsI 445 AAATTT [GA]^AATT[CT]
ApoI 445 AAATTT [GA]^AATT[CT]
BamHI 141 GGATCC G^GATCC
BanII 182 GAGCCC G[GA]GC[CT]^C
BcnI 30 CCGGG CC^[GC]GG
BmyI 182 GAGCCC G[AGT]GC[ACT]^C
BsmFI 32 GGGAC GGGAC(10/14)
Bsp1286I 182 GAGCCC G[AGT]GC[ACT]^C
BspHI 591 TCATGA T^CATGA
BstEII 267 GGTTACC G^GT[ACGT]ACC
BstNI 659 CCAGG CC^[AT]GG
BstPI 267 GGTTACC G^GT[ACGT]ACC
Cac8I 510 GCAGGC GC[ACGT]^[ACGT]GC
DraI 256 TTTAAA TTT^AAA
DsaI 454 CCACGG C^C[GA][CT]GG
EarI 429 GAAGAG CTCTTC(1/4)
EcoO65I 267 GGTTACC G^GT[ACGT]ACC
EcoRII 659 CCAGG ^CC[AT]GG
EcoT14I 263 CCAAGG C^C[AT][AT]GG
Ksp632I 429 GAAGAG CTCTTC(1/4)
MvaI 659 CCAGG CC^[AT]GG
NciI 30 CCGGG CC^[GC]GG
PpuMI 32 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 629 CAGCTG CAG^CTG
RcaI 591 TCATGA T^CATGA
SfaNI 229 GATGC GCATC(5/9)
StyI 263 CCAAGG C^C[AT][AT]GG
SwaI 255 ATTTAAAT ATTT^AAAT
TaqI 567 TCGA T^CGA
TfiI 460 GATTC G^A[AT]TC
Tsp45I 482 GTGAC ^GT[GC]AC
TspRI 365 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 567 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 525 GTAC GT^AC
580 GTAC
AflIII 326 ACGTGT A^C[GA][CT]GT
527 ACACGT
AvaII 33 GGACC G^G[AT]CC
503 GGACC
BsmAI 21 GAGAC GTCTC(1/5)
394 GAGAC
BsmBI 21 GAGACG CGTCTC(1/5)
394 GAGACG
BsrI 84 CCAGT ACTGG(1/-1)
621 CCAGT
BssKI 30 CCGGG ^CC[ACGT]GG
659 CCAGG
BstF5I 381 CATCC GGATG(2/0)
228 GGATG
BstYI 141 GGATCC [GA]^GATC[CT]
557 GGATCT
DdeI 114 CTCAG C^T[ACGT]AG
521 CTCAG
DraII 32 GGGACCC [GA]G^G[ACGT]CC[CT]
512 AGGCCCC
EcoO109I 32 GGGACCC [GA]G^G[ACGT]CC[CT]
512 AGGCCCC
FokI 381 CATCC GGATG(9/13)
228 GGATG
HaeIII 499 GGCC GG^CC
513 GGCC
HapII 30 CCGG C^CGG
501 CCGG
HpaII 30 CCGG C^CGG
501 CCGG
MaeIII 268 GTTAC ^GT[ACGT]AC
482 GTGAC
MboII 426 GAAGA GAAGA(8/7)
429 GAAGA
MflI 141 GGATCC [GA]^GATC[CT]
557 GGATCT
MseI 257 TTAA T^TAA
443 TTAA
MspA1I 70 CAGCGG C[AC]G^C[GT]G
629 CAGCTG
MspI 30 CCGG C^CGG
501 CCGG
PleI 595 GACTC GAGTC(4/5)
155 GAGTC
RsaI 525 GTAC GT^AC
580 GTAC
ScrFI 30 CCGGG CC^[ACGT]GG
659 CCAGG
Tru9I 257 TTAA T^TAA
443 TTAA
Tsp509I 387 AATT ^AATT
446 AATT
XhoII 141 GGATCC [GA]^GATC[CT]
557 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI Cfr13I DpnII FauI
Fnu4HI HinfI ItaI MaeII MboI
MnlI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I TseI