Restriction map for
pFN21AE9444
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI Asp718I AspEI AviII BanI
BbeI BbvCI BfrI BglI BlpI
Bpu1102I BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BstAPI
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I FseI FspI KasI KpnI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacI SalI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 145 GACGTC GACGT^C
AcyI 145 GACGTC G[GA]^CG[CT]C
AflIII 2586 ACATGT A^C[GA][CT]GT
AseI 3289 ATTAAT AT^TAAT
AsnI 3289 ATTAAT AT^TAAT
Asp700I 3955 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2062 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4260 CCTAGG C^CTAGG
BalI 4 TGGCCA TGG^CCA
BbrPI 2640 CACGTG CAC^GTG
BcgI 3415 CGAACACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4260 CCTAGG C^CTAGG
BsaHI 145 GACGTC G[GA]^CG[CT]C
BsaWI 1391 ACCGGA [AT]^CCGG[AT]
BseRI 3937 GAGGAG GAGGAG(10/8)
Bsp1407I 1809 TGTACA T^GTACA
BspLU11I 2586 ACATGT A^CATGT
BspMI 301 GCAGGT ACCTGC(4/8)
BsrGI 1809 TGTACA T^GTACA
BssSI 3934 CACGAG CACGAG(-5/-1)
BstBI 3545 TTCGAA TT^CGAA
BstEII 3667 GGTAACC G^GT[ACGT]ACC
BstPI 3667 GGTAACC G^GT[ACGT]ACC
DraIII 1147 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3163 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3667 GGTAACC G^GT[ACGT]ACC
EcoRI 953 GAATTC G^AATTC
EcoRV 1276 GATATC GAT^ATC
EcoT22I 1955 ATGCAT ATGCA^T
FauI 2708 GCGGG CCCGC(4/6)
Hin1I 145 GACGTC G[GA]^CG[CT]C
HpaI 1924 GTTAAC GTT^AAC
KspI 2706 CCGCGG CCGC^GG
MfeI 865 CAATTG C^AATTG
MluNI 4 TGGCCA TGG^CCA
MscI 4 TGGCCA TGG^CCA
MunI 865 CAATTG C^AATTG
NdeI 911 CATATG CA^TATG
NsiI 1955 ATGCAT ATGCA^T
NspV 3545 TTCGAA TT^CGAA
PflFI 2062 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2640 CACGTG CAC^GTG
PmlI 2640 CACGTG CAC^GTG
PshAI 322 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3289 ATTAAT AT^TAAT
SacII 2706 CCGCGG CCGC^GG
SbfI 1564 CCTGCAGG CCTGCA^GG
SfuI 3545 TTCGAA TT^CGAA
Sse8387I 1564 CCTGCAGG CCTGCA^GG
SspBI 1809 TGTACA T^GTACA
StuI 1797 AGGCCT AGG^CCT
Tth111I 2062 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3955 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 2707 CGCG CG^CG
4312 CGCG
AlwNI 302 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1641 CAGCAACTG
Aor51HI 1883 AGCGCT AGC^GCT
3827 AGCGCT
AspHI 2112 GAGCAC G[AT]GC[AT]^C
4093 GAGCAC
AvaI 1508 CCCGAG C^[CT]CG[GA]G
4363 CCCGAG
BamHI 1620 GGATCC G^GATCC
4046 GGATCC
BanII 1347 GAGCCC G[GA]GC[CT]^C
2710 GGGCTC
BclI 1980 TGATCA T^GATCA
2636 TGATCA
BcnI 1776 CCGGG CC^[GC]GG
1853 CCCGG
BglII 1635 AGATCT A^GATCT
4104 AGATCT
BsaAI 2640 CACGTG [CT]AC^GT[GA]
2933 CACGTA
BsaBI 2679 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2784 GATTGGGATC
BsaI 1964 GAGACC GGTCTC(1/5)
3754 GAGACC
BsiHKAI 2112 GAGCAC G[AT]GC[AT]^C
4093 GAGCAC
BsoBI 1508 CCCGAG C^[CT]CG[GA]G
4363 CCCGAG
BsrFI 4209 ACCGGC [GA]^CCGG[CT]
4383 ACCGGC
Bst1107I 424 GTATAC GTA^TAC
4000 GTATAC
BstUI 2707 CGCG CG^CG
4312 CGCG
BstXI 456 CCACCAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3902 CCATTGTCCTGG
BstZ17I 424 GTATAC GTA^TAC
4000 GTATAC
Cfr10I 4209 ACCGGC [GA]^CCGG[CT]
4383 ACCGGC
Eco47III 1883 AGCGCT AGC^GCT
3827 AGCGCT
FbaI 1980 TGATCA T^GATCA
2636 TGATCA
HaeII 1883 AGCGCT [GA]GCGC^[CT]
3827 AGCGCT
HgaI 3491 GACGC GACGC(5/10)
3595 GCGTC
HincII 1924 GTTAAC GT[CT]^[GA]AC
2496 GTCAAC
HindII 1924 GTTAAC GT[CT]^[GA]AC
2496 GTCAAC
MamI 2679 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2784 GATTGGGATC
MvnI 2707 CGCG CG^CG
4312 CGCG
NciI 1776 CCGGG CC^[GC]GG
1853 CCCGG
PflMI 1905 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3658 CCACTGAATGG
SapI 2775 GCTCTTC GCTCTTC(1/4)
3241 GCTCTTC
ScaI 1396 AGTACT AGT^ACT
2574 AGTACT
SpeI 1399 ACTAGT A^CTAGT
4399 ACTAGT
SphI 4142 GCATGC GCATG^C
4250 GCATGC
SspI 1157 AATATT AAT^ATT
2086 AATATT
Van91I 1905 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3658 CCACTGAATGG
XcmI 1781 CCATGGACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2279 CCAGTATGAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII BbsI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII