Restriction map for
pFN21AE9679
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# NO CUT ENZYMES ( 68 enzymes) #
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AatII AgeI AhdI Aor51HI ApaI
AscI AspEI AspI AviII BbeI
BbrPI BcgI BglI BlpI Bpu1102I
BsiEI BsrBI BssHII Bst1107I BstEII
BstPI BstZ17I CelII CfoI CpoI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI HaeII
HhaI HinP1I KasI KspI MluI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 4983 GGTACC G^GTACC
AccII 6663 CGCG CG^CG
AccIII 4439 TCCGGA T^CCGGA
AclI 2800 AACGTT AA^CGTT
AcyI 5357 GGCGTC G[GA]^CG[CT]C
Alw44I 1069 GTGCAC G^TGCAC
AocI 1033 CCTCAGG CC^T[ACGT]AGG
ApaLI 1069 GTGCAC G^TGCAC
AseI 7423 ATTAAT AT^TAAT
AsnI 7423 ATTAAT AT^TAAT
Asp718I 4983 GGTACC G^GTACC
AvrII 6094 CCTAGG C^CTAGG
BalI 6775 TGGCCA TGG^CCA
BbvCI 3457 GCTGAGG CCTCAGC(-5/-2)
BciVI 2361 GGATAC GTATCC(6/5)
BlnI 6094 CCTAGG C^CTAGG
BsaAI 2784 TACGTG [CT]AC^GT[GA]
BsaHI 5357 GGCGTC G[GA]^CG[CT]C
BseAI 4439 TCCGGA T^CCGGA
BsgI 125 GTGCAG GTGCAG(16/14)
BsiWI 2782 CGTACG C^GTACG
BspDI 4135 ATCGAT AT^CGAT
BspEI 4439 TCCGGA T^CCGGA
BstUI 6663 CGCG CG^CG
Bsu36I 1033 CCTCAGG CC^T[ACGT]AGG
ClaI 4135 ATCGAT AT^CGAT
DrdI 6720 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1033 CCTCAGG CC^T[ACGT]AGG
EcoRV 4132 GATATC GAT^ATC
FauI 7842 GCGGG CCCGC(4/6)
Hin1I 5357 GGCGTC G[GA]^CG[CT]C
HpaI 628 GTTAAC GTT^AAC
KpnI 4983 GGTACC GGTAC^C
MluNI 6775 TGGCCA TGG^CCA
MroI 4439 TCCGGA T^CCGGA
MscI 6775 TGGCCA TGG^CCA
MvnI 6663 CGCG CG^CG
PshAI 4187 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 7423 ATTAAT AT^TAAT
Psp1406I 2800 AACGTT AA^CGTT
SexAI 2013 ACCTGGT A^CC[AT]GGT
SmaI 7503 CCCGGG CCC^GGG
SpeI 102 ACTAGT A^CTAGT
XmaCI 7503 CCCGGG C^CCGGG
XmaI 7503 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 8279 CCGC CCGC(-3/-1)
7842 GCGG
AflII 3135 CTTAAG C^TTAAG
7492 CTTAAG
BamHI 2222 GGATCC G^GATCC
5721 GGATCC
BfrI 3135 CTTAAG C^TTAAG
7492 CTTAAG
BsaWI 4232 ACCGGA [AT]^CCGG[AT]
4439 TCCGGA
Bsp1407I 81 TGTACA T^GTACA
8433 TGTACA
BspMI 4502 ACCTGC ACCTGC(4/8)
5214 ACCTGC
BsrDI 2405 CATTGC GCAATG(2/0)
3526 GCAATG
BsrFI 6964 GCCGGT [GA]^CCGG[CT]
8041 ACCGGC
BsrGI 81 TGTACA T^GTACA
8433 TGTACA
BssSI 1637 CACGAG CACGAG(-5/-1)
4694 CACGAG
BstBI 4067 TTCGAA TT^CGAA
4805 TTCGAA
Cfr10I 6964 GCCGGT [GA]^CCGG[CT]
8041 ACCGGC
DraI 5062 TTTAAA TTT^AAA
5230 TTTAAA
DraIII 3058 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
5929 CACGGGGTG
EaeI 4548 TGGCCG [CT]^GGCC[GA]
6775 TGGCCA
NspV 4067 TTCGAA TT^CGAA
4805 TTCGAA
SacI 3415 GAGCTC GAGCT^C
4598 GAGCTC
SapI 1467 GAAGAGC GCTCTTC(1/4)
2197 GAAGAGC
ScaI 2422 AGTACT AGT^ACT
6625 AGTACT
SfuI 4067 TTCGAA TT^CGAA
4805 TTCGAA
SspBI 81 TGTACA T^GTACA
8433 TGTACA
XbaI 2092 TCTAGA T^CTAGA
6123 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
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AccI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspHI BspLU11I BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI