Restriction map for
pFN21AE4159
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BclI BcnI BglI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspEI BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I CpoI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRV FauI FbaI FseI
FspI HincII HindII HpaI KasI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 68 enzymes) #
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Acc65I 869 GGTACC G^GTACC
AcyI 1193 GGCGTC G[GA]^CG[CT]C
AflII 1318 CTTAAG C^TTAAG
AflIII 759 ACATGT A^C[GA][CT]GT
Aor51HI 515 AGCGCT AGC^GCT
Asp718I 869 GGTACC G^GTACC
BanI 869 GGTACC G^G[CT][GA]CC
BanII 748 GAGCTC G[GA]GC[CT]^C
BfrI 1318 CTTAAG C^TTAAG
BlpI 1036 GCTAAGC GC^T[ACGT]AGC
BpmI 157 CTCCAG CTGGAG(16/14)
Bpu1102I 1036 GCTAAGC GC^T[ACGT]AGC
BsaAI 1576 CACGTA [CT]AC^GT[GA]
BsaHI 1193 GGCGTC G[GA]^CG[CT]C
BsgI 1454 GTGCAG GTGCAG(16/14)
Bsp1407I 405 TGTACA T^GTACA
BspDI 1295 ATCGAT AT^CGAT
BspHI 108 TCATGA T^CATGA
BspLU11I 759 ACATGT A^CATGT
BspMI 917 GCAGGT ACCTGC(4/8)
BsrDI 1729 GCAATG GCAATG(2/0)
BsrFI 1519 GCCGGT [GA]^CCGG[CT]
BsrGI 405 TGTACA T^GTACA
BssKI 1644 CCTGG ^CC[ACGT]GG
BssSI 745 CACGAG CACGAG(-5/-1)
BstAPI 1069 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 1644 CCTGG CC^[AT]GG
BstYI 36 GGATCT [GA]^GATC[CT]
CelII 1036 GCTAAGC GC^T[ACGT]AGC
CfoI 516 GCGC GCG^C
Cfr10I 1519 GCCGGT [GA]^CCGG[CT]
ClaI 1295 ATCGAT AT^CGAT
DraI 1792 TTTAAA TTT^AAA
DraII 1383 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 993 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 515 AGCGCT AGC^GCT
EcoNI 1009 CCTGTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1383 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 828 GAATTC G^AATTC
EcoRII 1644 CCTGG ^CC[AT]GG
EcoT22I 458 ATGCAT ATGCA^T
HaeII 515 AGCGCT [GA]GCGC^[CT]
HapII 1520 CCGG C^CGG
HhaI 516 GCGC GCG^C
Hin1I 1193 GGCGTC G[GA]^CG[CT]C
HinP1I 516 GCGC G^CGC
HindIII 1039 AAGCTT A^AGCTT
HpaII 1520 CCGG C^CGG
KpnI 869 GGTACC GGTAC^C
MaeII 1577 ACGT A^CGT
MflI 36 GGATCT [GA]^GATC[CT]
MspA1I 1487 CAGCTG C[AC]G^C[GT]G
MspI 1520 CCGG C^CGG
MvaI 1644 CCTGG CC^[AT]GG
NsiI 458 ATGCAT ATGCA^T
PflMI 1436 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 767 GACTC GAGTC(4/5)
PpuMI 1383 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1340 CTGCAG CTGCA^G
PvuII 1487 CAGCTG CAG^CTG
RcaI 108 TCATGA T^CATGA
SacI 748 GAGCTC GAGCT^C
ScrFI 1644 CCTGG CC^[ACGT]GG
SspBI 405 TGTACA T^GTACA
TaqI 1296 TCGA T^CGA
TthHB8I 1296 TCGA T^CGA
Van91I 1436 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 36 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 186 CCGC CCGC(-3/-1)
1649 GCGG
Asp700I 604 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1584 GAATACATTC
BbsI 731 GAAGAC GAAGAC(2/6)
1652 GAAGAC
BciVI 592 GTATCC GTATCC(6/5)
1492 GTATCC
BfaI 908 CTAG C^TAG
1349 CTAG
BpuAI 731 GAAGAC GAAGAC(2/6)
1652 GAAGAC
BseRI 478 GAGGAG GAGGAG(10/8)
676 GAGGAG
BsiYI 1009 CCTGTAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1436 CCAATTCTTGG
BslI 1009 CCTGTAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1436 CCAATTCTTGG
BsmAI 421 GAGAC GTCTC(1/5)
765 GAGAC
BsmFI 15 GGGAC GGGAC(10/14)
897 GTCCC
BstXI 467 CCAAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
873 CCACATACTTGG
EarI 126 CTCTTC CTCTTC(1/4)
547 CTCTTC
HaeIII 911 GGCC GG^CC
1739 GGCC
HgaI 17 GACGC GACGC(5/10)
1194 GCGTC
HphI 384 GGTGA GGTGA(8/7)
939 GGTGA
Ksp632I 126 CTCTTC CTCTTC(1/4)
547 CTCTTC
MaeI 908 CTAG C^TAG
1349 CTAG
MfeI 817 CAATTG C^AATTG
1466 CAATTG
MslI 207 CATTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1753 CATGGCTGTG
MunI 817 CAATTG C^AATTG
1466 CAATTG
MwoI 1069 GCAGTCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1352 GCTGTGTAGGC
NlaIV 87 GGACCC GG[ACGT]^[ACGT]CC
869 GGTACC
NspI 460 GCATGT [GA]CATG^[CT]
759 ACATGT
SmlI 613 CTTGAG C^T[CT][GA]AG
1318 CTTAAG
SspI 9 AATATT AAT^ATT
1153 AATATT
XmnI 604 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1584 GAATACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BbvI BmyI BsaJI
BsiHKAI Bsp1286I BsrI BstF5I Cfr13I
DdeI DpnII EcoT14I Fnu4HI FokI
HinfI ItaI MaeIII MboI MboII
MnlI MseI NdeII NlaIII RsaI
Sau3AI Sau96I SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI