Restriction map for
pFN21AE3070
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsiYI BslI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaI HpaII HphI KasI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI TfiI Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 486 GCGG CCGC(-3/-1)
AflIII 368 ACATGT A^C[GA][CT]GT
AlwNI 11 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 507 GAGCAC G[AT]GC[AT]^C
AvaII 244 GGACC G^G[AT]CC
BanI 387 GGCACC G^G[CT][GA]CC
BbsI 547 GTCTTC GAAGAC(2/6)
BciVI 612 GGATAC GTATCC(6/5)
BmyI 507 GAGCAC G[AGT]GC[ACT]^C
BpuAI 547 GTCTTC GAAGAC(2/6)
BsaJI 320 CCTTGG C^C[ACGT][ACGT]GG
BseRI 377 GAGGAG GAGGAG(10/8)
BsgI 140 CTGCAC GTGCAG(16/14)
BsiHKAI 507 GAGCAC G[AT]GC[AT]^C
BsmAI 536 GAGAC GTCTC(1/5)
BsmFI 243 GGGAC GGGAC(10/14)
Bsp1286I 507 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 368 ACATGT A^CATGT
BspMI 10 GCAGGT ACCTGC(4/8)
BsrDI 492 CATTGC GCAATG(2/0)
BsrI 542 ACTGG ACTGG(1/-1)
BssKI 258 CCTGG ^CC[ACGT]GG
BstAPI 10 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 258 CCTGG CC^[AT]GG
BstXI 233 CCAACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 176 GGATCT [GA]^GATC[CT]
Cac8I 187 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 244 GGACC G^G[ACGT]CC
DdeI 194 CTGAG C^T[ACGT]AG
DraI 211 TTTAAA TTT^AAA
DraII 243 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 538 GACAACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 302 GAAGAG CTCTTC(1/4)
EcoO109I 243 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 258 CCTGG ^CC[AT]GG
EcoT14I 320 CCTTGG C^C[AT][AT]GG
EcoT22I 660 ATGCAT ATGCA^T
HindIII 185 AAGCTT A^AGCTT
Ksp632I 302 GAAGAG CTCTTC(1/4)
MflI 176 GGATCT [GA]^GATC[CT]
MspA1I 137 CAGCTG C[AC]G^C[GT]G
MvaI 258 CCTGG CC^[AT]GG
NlaIII 369 CATG CATG^
NsiI 660 ATGCAT ATGCA^T
NspI 368 ACATGT [GA]CATG^[CT]
PpuMI 243 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 137 CAGCTG CAG^CTG
Sau96I 244 GGACC G^G[ACGT]CC
ScrFI 258 CCTGG CC^[ACGT]GG
SmlI 71 CTCAAG C^T[CT][GA]AG
SspI 359 AATATT AAT^ATT
StyI 320 CCTTGG C^C[AT][AT]GG
TspRI 41 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 248 CCAAAATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 176 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 3 enzymes) #
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AlwI 176 GGATC GGATC(4/5)
308 GGATC
HaeIII 411 GGCC GG^CC
635 GGCC
MaeII 144 ACGT A^CGT
236 ACGT
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BbvI BstF5I DpnII Fnu4HI
FokI ItaI MboI MboII MnlI
MseI MwoI NdeII NlaIV Sau3AI
SfaNI Tru9I TseI