Restriction map for
pFN21AB6220
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
ApoI AseI AsnI Asp718I AspEI
AspI BbeI BbrPI BbsI BciVI
BfrI BpuAI BsaAI BsaBI BsaHI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FseI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MroI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI ScaI SexAI SfoI
SfuI SnaBI SpeI SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflIII 1837 ACGCGT A^C[GA][CT]GT
AscI 1487 GGCGCGCC GG^CGCGCC
Asp700I 2118 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 511 CCTAGG C^CTAGG
BamHI 960 GGATCC G^GATCC
BanI 1118 GGCACC G^G[CT][GA]CC
BbvCI 1113 GCTGAGG CCTCAGC(-5/-2)
BcgI 336 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 989 TGATCA T^GATCA
BglI 860 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 674 AGATCT A^GATCT
BlnI 511 CCTAGG C^CTAGG
BlpI 1408 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1408 GCTAAGC GC^T[ACGT]AGC
BsaI 1908 GGTCTC GGTCTC(1/5)
BsaWI 140 TCCGGT [AT]^CCGG[AT]
BsmAI 1909 GTCTC GTCTC(1/5)
CelII 1408 GCTAAGC GC^T[ACGT]AGC
EarI 1282 GAAGAG CTCTTC(1/4)
FbaI 989 TGATCA T^GATCA
Ksp632I 1282 GAAGAG CTCTTC(1/4)
MaeII 833 ACGT A^CGT
MluI 1837 ACGCGT A^CGCGT
NotI 1166 GCGGCCGC GC^GGCCGC
SapI 1282 GAAGAGC GCTCTTC(1/4)
SbfI 540 CCTGCAGG CCTGCA^GG
SfiI 859 GGCCTTTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1395 CCCGGG CCC^GGG
SmlI 1828 CTCAAG C^T[CT][GA]AG
Sse8387I 540 CCTGCAGG CCTGCA^GG
TaqI 1547 TCGA T^CGA
TfiI 526 GAATC G^A[AT]TC
Tsp45I 397 GTCAC ^GT[GC]AC
TthHB8I 1547 TCGA T^CGA
XcmI 684 CCACAGCAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1395 CCCGGG C^CCGGG
XmaI 1395 CCCGGG C^CCGGG
XmnI 2118 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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Alw44I 1297 GTGCAC G^TGCAC
1600 GTGCAC
AocI 637 CCTGAGG CC^T[ACGT]AGG
700 CCTGAGG
ApaLI 1297 GTGCAC G^TGCAC
1600 GTGCAC
BstXI 648 CCAGAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2133 CCAGGACTGTGG
Bsu36I 637 CCTGAGG CC^T[ACGT]AGG
700 CCTGAGG
DsaI 950 CCACGG C^C[GA][CT]GG
1383 CCACGG
Eco81I 637 CCTGAGG CC^T[ACGT]AGG
700 CCTGAGG
EcoNI 633 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
710 CCTCATGGAGG
EcoT14I 236 CCAAGG C^C[AT][AT]GG
511 CCTAGG
MaeIII 397 GTCAC ^GT[ACGT]AC
565 GTTAC
NspI 906 GCATGC [GA]CATG^[CT]
2044 GCATGC
PflMI 487 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
995 CCACCCGCTGG
SphI 906 GCATGC GCATG^C
2044 GCATGC
StyI 236 CCAAGG C^C[AT][AT]GG
511 CCTAGG
Van91I 487 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
995 CCACCCGCTGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Aor51HI ApaI AspHI AvaI
AvaII AviII BalI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EclXI
Eco47III Eco52I EcoO109I EcoRII FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SgrAI
StuI TseI TspRI XhoII