Restriction map for
pFN21AB0406
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MaeII MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Alw44I 875 GTGCAC G^TGCAC
AocI 851 CCTCAGG CC^T[ACGT]AGG
ApaI 968 GGGCCC GGGCC^C
ApaLI 875 GTGCAC G^TGCAC
AvaI 1043 CTCGAG C^[CT]CG[GA]G
BalI 1530 TGGCCA TGG^CCA
BamHI 1788 GGATCC G^GATCC
BciVI 1857 GTATCC GTATCC(6/5)
BsaWI 222 ACCGGA [AT]^CCGG[AT]
BseRI 337 GAGGAG GAGGAG(10/8)
BsgI 1513 CTGCAC GTGCAG(16/14)
BsoBI 1043 CTCGAG C^[CT]CG[GA]G
BstBI 2238 TTCGAA TT^CGAA
BstXI 2096 CCAATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 851 CCTCAGG CC^T[ACGT]AGG
CfoI 135 GCGC GCG^C
DraI 802 TTTAAA TTT^AAA
DsaI 564 CCACGG C^C[GA][CT]GG
EaeI 1530 TGGCCA [CT]^GGCC[GA]
EarI 2007 GAAGAG CTCTTC(1/4)
Eco81I 851 CCTCAGG CC^T[ACGT]AGG
EcoNI 285 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2318 ATGCAT ATGCA^T
FauI 971 CCCGC CCCGC(4/6)
HapII 223 CCGG C^CGG
HgaI 522 GCGTC GACGC(5/10)
HhaI 135 GCGC GCG^C
HinP1I 135 GCGC G^CGC
HincII 2171 GTCAAC GT[CT]^[GA]AC
HindII 2171 GTCAAC GT[CT]^[GA]AC
HindIII 8 AAGCTT A^AGCTT
HpaII 223 CCGG C^CGG
Ksp632I 2007 GAAGAG CTCTTC(1/4)
MluNI 1530 TGGCCA TGG^CCA
MscI 1530 TGGCCA TGG^CCA
MslI 952 CACCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 223 CCGG C^CGG
NdeI 1993 CATATG CA^TATG
NsiI 2318 ATGCAT ATGCA^T
NspV 2238 TTCGAA TT^CGAA
PaeR7I 1043 CTCGAG C^TCGAG
PflMI 639 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 634 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 295 GAGCTC GAGCT^C
ScaI 895 AGTACT AGT^ACT
SexAI 1460 ACCTGGT A^CC[AT]GGT
SfuI 2238 TTCGAA TT^CGAA
SphI 974 GCATGC GCATG^C
Van91I 639 CCAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1043 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 129 CCGC CCGC(-3/-1)
972 CCGC
AcsI 1593 AAATTT [GA]^AATT[CT]
1706 AAATTT
ApoI 1593 AAATTT [GA]^AATT[CT]
1706 AAATTT
BanII 295 GAGCTC G[GA]GC[CT]^C
968 GGGCCC
BbsI 312 GAAGAC GAAGAC(2/6)
651 GAAGAC
BpuAI 312 GAAGAC GAAGAC(2/6)
651 GAAGAC
BsaBI 1783 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1789 GATCCTGATC
BsaI 219 GAGACC GGTCTC(1/5)
1566 GAGACC
BsmI 1651 GAATGC GAATGC(1/-1)
2200 GAATGC
BspMI 1511 ACCTGC ACCTGC(4/8)
81 GCAGGT
DraII 18 AGGCCCT [GA]G^G[ACGT]CC[CT]
634 AGGTCCC
EcoO109I 18 AGGCCCT [GA]G^G[ACGT]CC[CT]
634 AGGTCCC
MamI 1783 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1789 GATCCTGATC
NspI 422 ACATGC [GA]CATG^[CT]
974 GCATGC
PstI 683 CTGCAG CTGCA^G
1001 CTGCAG
StuI 395 AGGCCT AGG^CCT
1065 AGGCCT
TaqI 1044 TCGA T^CGA
2239 TCGA
TthHB8I 1044 TCGA T^CGA
2239 TCGA
XbaI 363 TCTAGA T^CTAGA
2358 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AfaI AluI AlwI AlwNI AspHI
AvaII BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII