Restriction map for
pFN21AB5860
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# NO CUT ENZYMES ( 147 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspHI AspI AvaI AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BglI BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I FauI FseI
HaeII HapII HgaI HindIII HpaII
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 257 GACGTC GACGT^C
AciI 94 CCGC CCGC(-3/-1)
AcyI 257 GACGTC G[GA]^CG[CT]C
AflII 867 CTTAAG C^TTAAG
AhdI 856 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 341 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1091 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 856 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 558 GGACC G^G[AT]CC
AviII 123 TGCGCA TGC^GCA
BclI 198 TGATCA T^GATCA
BfrI 867 CTTAAG C^TTAAG
BglII 234 AGATCT A^GATCT
BpmI 850 CTGGAG CTGGAG(16/14)
BsaHI 257 GACGTC G[GA]^CG[CT]C
BsaI 1213 GAGACC GGTCTC(1/5)
BseRI 571 GAGGAG GAGGAG(10/8)
BsgI 416 GTGCAG GTGCAG(16/14)
BsiYI 1330 CCACGAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1330 CCACGAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 1334 GAGACG CGTCTC(1/5)
BsmI 922 GAATGC GAATGC(1/-1)
BspMI 118 GCAGGT ACCTGC(4/8)
BsrI 252 ACTGG ACTGG(1/-1)
BssSI 1331 CACGAG CACGAG(-5/-1)
BstAPI 115 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 891 GGTTACC G^GT[ACGT]ACC
BstPI 891 GGTTACC G^GT[ACGT]ACC
CfoI 124 GCGC GCG^C
Eam1105I 856 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 891 GGTTACC G^GT[ACGT]ACC
EcoRI 1177 GAATTC G^AATTC
EcoT22I 492 ATGCAT ATGCA^T
FbaI 198 TGATCA T^GATCA
FspI 123 TGCGCA TGC^GCA
HhaI 124 GCGC GCG^C
Hin1I 257 GACGTC G[GA]^CG[CT]C
HinP1I 124 GCGC G^CGC
HincII 1008 GTTAAC GT[CT]^[GA]AC
HindII 1008 GTTAAC GT[CT]^[GA]AC
HpaI 1008 GTTAAC GTT^AAC
MslI 177 CATAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1444 CAGCTG C[AC]G^C[GT]G
NdeI 214 CATATG CA^TATG
NlaIV 1101 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 492 ATGCAT ATGCA^T
NspI 111 GCATGC [GA]CATG^[CT]
PvuII 1444 CAGCTG CAG^CTG
SphI 111 GCATGC GCATG^C
TaqI 1086 TCGA T^CGA
TthHB8I 1086 TCGA T^CGA
XbaI 502 TCTAGA T^CTAGA
XcmI 1217 CCATCTCAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1091 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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BsaJI 561 CCTTGG C^C[ACGT][ACGT]GG
595 CCTTGG
BsmAI 1213 GAGAC GTCTC(1/5)
1334 GAGAC
BsmFI 255 GGGAC GGGAC(10/14)
1075 GTCCC
BssKI 61 CCTGG ^CC[ACGT]GG
849 CCTGG
BstNI 61 CCTGG CC^[AT]GG
849 CCTGG
BstYI 53 GGATCT [GA]^GATC[CT]
234 AGATCT
EcoRII 61 CCTGG ^CC[AT]GG
849 CCTGG
EcoRV 460 GATATC GAT^ATC
1033 GATATC
EcoT14I 561 CCTTGG C^C[AT][AT]GG
595 CCTTGG
MaeII 258 ACGT A^CGT
1027 ACGT
MflI 53 GGATCT [GA]^GATC[CT]
234 AGATCT
MvaI 61 CCTGG CC^[AT]GG
849 CCTGG
ScrFI 61 CCTGG CC^[ACGT]GG
849 CCTGG
SfaNI 343 GATGC GCATC(5/9)
1123 GATGC
StyI 561 CCTTGG C^C[AT][AT]GG
595 CCTTGG
XhoII 53 GGATCT [GA]^GATC[CT]
234 AGATCT
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BfaI BpuAI BstF5I
Cac8I Cfr13I DdeI DpnII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MwoI NdeII NlaIII PleI
RsaI Sau3AI Sau96I SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI