Restriction map for
pFN21AB6210
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII Acc65I AccI AclI AcsI
AgeI AhdI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BamHI BbrPI BbsI BcgI
BciVI BclI BglII BlnI BpuAI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI HincII HindII HindIII
HpaI KpnI KspI MaeIII MamI
MfeI MluNI MscI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
TfiI Tsp45I Tsp509I Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 813 TCCGGA T^CCGGA
AfaI 2068 GTAC GT^AC
AflII 949 CTTAAG C^TTAAG
AflIII 2045 ACGCGT A^C[GA][CT]GT
AlwI 72 GGATC GGATC(4/5)
AlwNI 779 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1276 CCTGAGG CC^T[ACGT]AGG
Asp700I 189 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfrI 949 CTTAAG C^TTAAG
BsaAI 335 CACGTA [CT]AC^GT[GA]
BsaI 2080 GAGACC GGTCTC(1/5)
BseAI 813 TCCGGA T^CCGGA
BsmBI 1739 CGTCTC CGTCTC(1/5)
BspEI 813 TCCGGA T^CCGGA
BstAPI 546 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 974 CCAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 72 GGATCT [GA]^GATC[CT]
Bsu36I 1276 CCTGAGG CC^T[ACGT]AGG
DraIII 989 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 633 CCACGG C^C[GA][CT]GG
Eco81I 1276 CCTGAGG CC^T[ACGT]AGG
HinfI 147 GAGTC G^A[ACGT]TC
MflI 72 GGATCT [GA]^GATC[CT]
MluI 2045 ACGCGT A^CGCGT
MroI 813 TCCGGA T^CCGGA
MseI 950 TTAA T^TAA
MslI 960 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 920 GCGGCCGC GC^GGCCGC
PleI 147 GAGTC GAGTC(4/5)
PstI 76 CTGCAG CTGCA^G
RsaI 2068 GTAC GT^AC
SacI 1975 GAGCTC GAGCT^C
SapI 370 GAAGAGC GCTCTTC(1/4)
SfaNI 1545 GCATC GCATC(5/9)
SphI 425 GCATGC GCATG^C
Tru9I 950 TTAA T^TAA
XcmI 443 CCACTGAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 72 GGATCT [GA]^GATC[CT]
XmnI 189 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 1480 GGCGCC G[GA]^CG[CT]C
1768 GGCGCC
Aor51HI 191 AGCGCT AGC^GCT
1077 AGCGCT
AviII 311 TGCGCA TGC^GCA
1847 TGCGCA
BbeI 1480 GGCGCC GGCGC^C
1768 GGCGCC
BfaI 1793 CTAG C^TAG
2018 CTAG
BglI 631 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1130 GCCTCTTGGGC
BlpI 1186 GCTGAGC GC^T[ACGT]AGC
1523 GCTCAGC
Bpu1102I 1186 GCTGAGC GC^T[ACGT]AGC
1523 GCTCAGC
BsaHI 1480 GGCGCC G[GA]^CG[CT]C
1768 GGCGCC
BsaWI 813 TCCGGA [AT]^CCGG[AT]
912 TCCGGT
BsmAI 2080 GAGAC GTCTC(1/5)
1740 GTCTC
BssHII 375 GCGCGC G^CGCGC
377 GCGCGC
BstF5I 549 CATCC GGATG(2/0)
122 GGATG
CelII 1186 GCTGAGC GC^T[ACGT]AGC
1523 GCTCAGC
Eco47III 191 AGCGCT AGC^GCT
1077 AGCGCT
FokI 549 CATCC GGATG(9/13)
122 GGATG
FseI 1165 GGCCGGCC GGCCGG^CC
1665 GGCCGGCC
FspI 311 TGCGCA TGC^GCA
1847 TGCGCA
Hin1I 1480 GGCGCC G[GA]^CG[CT]C
1768 GGCGCC
KasI 1480 GGCGCC G^GCGCC
1768 GGCGCC
MaeI 1793 CTAG C^TAG
2018 CTAG
NarI 1480 GGCGCC GG^CGCC
1768 GGCGCC
NspI 425 GCATGC [GA]CATG^[CT]
875 ACATGC
SfcI 76 CTGCAG C^T[GA][CT]AG
1633 CTACAG
SfoI 1480 GGCGCC GGC^GCC
1768 GGCGCC
StuI 248 AGGCCT AGG^CCT
890 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AluI Alw44I ApaI
ApaLI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PpuMI
Sau3AI Sau96I ScrFI SmaI SmlI
TaqI TseI TspRI TthHB8I XmaCI
XmaI