Restriction map for
pFN21AB0472
##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII Aor51HI AscI AseI
AsnI Asp700I Asp718I AvrII BbeI
BclI BfaI BfrI BlnI BsaBI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssSI Bst1107I BstZ17I ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I FbaI FseI HindIII
HpaI KasI KpnI MaeI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI PacI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 393 GAATTC [GA]^AATT[CT]
AgeI 2395 ACCGGT A^CCGGT
AhdI 1513 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 393 GAATTC [GA]^AATT[CT]
AspEI 1513 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 901 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2436 TGCGCA TGC^GCA
BbrPI 1819 CACGTG CAC^GTG
BbsI 1869 GAAGAC GAAGAC(2/6)
BcgI 1295 CGAGGGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 154 GTATCC GTATCC(6/5)
BlpI 2937 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2937 GCTCAGC GC^T[ACGT]AGC
BpuAI 1869 GAAGAC GAAGAC(2/6)
BsaAI 1819 CACGTG [CT]AC^GT[GA]
BsaI 1611 GGTCTC GGTCTC(1/5)
BsrDI 1602 CATTGC GCAATG(2/0)
BssHII 2528 GCGCGC G^CGCGC
BstAPI 2059 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 956 TTCGAA TT^CGAA
CelII 2937 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1513 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 393 GAATTC G^AATTC
EcoT22I 1859 ATGCAT ATGCA^T
FspI 2436 TGCGCA TGC^GCA
HaeII 758 AGCGCC [GA]GCGC^[CT]
HincII 1849 GTCAAC GT[CT]^[GA]AC
HindII 1849 GTCAAC GT[CT]^[GA]AC
KspI 221 CCGCGG CCGC^GG
NsiI 1859 ATGCAT ATGCA^T
NspI 1328 GCATGC [GA]CATG^[CT]
NspV 956 TTCGAA TT^CGAA
PflFI 901 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1566 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2395 ACCGGT A^CCGGT
PmaCI 1819 CACGTG CAC^GTG
PmlI 1819 CACGTG CAC^GTG
PshAI 318 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 221 CCGCGG CCGC^GG
SapI 229 GCTCTTC GCTCTTC(1/4)
SexAI 902 ACCAGGT A^CC[AT]GGT
SfuI 956 TTCGAA TT^CGAA
SmaI 320 CCCGGG CCC^GGG
SphI 1328 GCATGC GCATG^C
StuI 2561 AGGCCT AGG^CCT
Tth111I 901 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1566 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 320 CCCGGG C^CCGGG
XmaI 320 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 1814 GACGCC G[GA]^CG[CT]C
2713 GGCGTC
Alw44I 1657 GTGCAC G^TGCAC
2703 GTGCAC
AocI 1413 CCTGAGG CC^T[ACGT]AGG
2564 CCTCAGG
ApaI 2354 GGGCCC GGGCC^C
2897 GGGCCC
ApaLI 1657 GTGCAC G^TGCAC
2703 GTGCAC
BalI 302 TGGCCA TGG^CCA
2210 TGGCCA
BamHI 1157 GGATCC G^GATCC
2875 GGATCC
BanI 1406 GGCACC G^G[CT][GA]CC
1664 GGCACC
BglII 517 AGATCT A^GATCT
2625 AGATCT
BsaHI 1814 GACGCC G[GA]^CG[CT]C
2713 GGCGTC
BsgI 2497 CTGCAC GTGCAG(16/14)
1318 GTGCAG
BsmBI 62 GAGACG CGTCTC(1/5)
1447 GAGACG
BstEII 192 GGTCACC G^GT[ACGT]ACC
324 GGTCACC
BstPI 192 GGTCACC G^GT[ACGT]ACC
324 GGTCACC
Bsu36I 1413 CCTGAGG CC^T[ACGT]AGG
2564 CCTCAGG
DrdI 1108 GACGTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1930 GACGATATTGTC
Eco81I 1413 CCTGAGG CC^T[ACGT]AGG
2564 CCTCAGG
EcoNI 1164 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1275 CCTGGCTGAGG
EcoO65I 192 GGTCACC G^GT[ACGT]ACC
324 GGTCACC
EcoRV 259 GATATC GAT^ATC
883 GATATC
Hin1I 1814 GACGCC G[GA]^CG[CT]C
2713 GGCGTC
MluNI 302 TGGCCA TGG^CCA
2210 TGGCCA
MscI 302 TGGCCA TGG^CCA
2210 TGGCCA
NcoI 636 CCATGG C^CATGG
2787 CCATGG
TfiI 783 GATTC G^A[AT]TC
2828 GAATC
XcmI 636 CCATGGACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
731 CCAACATCGCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvCI BbvI BcnI BglI BmyI
BpmI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PaeR7I PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoI XhoII