Restriction map for
pFN21AE3252
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflIII AgeI Aor51HI
AscI Asp718I AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BclI BcnI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsoBI
Bsp1407I BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I CfoI CpoI DraII
DrdI EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 406 GTAGAC GT^[AC][GT]AC
AflII 660 CTTAAG C^TTAAG
AhdI 1134 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1253 GTGCAC G^TGCAC
ApaI 1608 GGGCCC GGGCC^C
ApaLI 1253 GTGCAC G^TGCAC
AseI 331 ATTAAT AT^TAAT
AsnI 331 ATTAAT AT^TAAT
AspEI 1134 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1749 GGATCC G^GATCC
BcgI 275 GCAAAATTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 387 GTATCC GTATCC(6/5)
BfrI 660 CTTAAG C^TTAAG
BlpI 850 GCTCAGC GC^T[ACGT]AGC
BpmI 1036 CTGGAG CTGGAG(16/14)
Bpu1102I 850 GCTCAGC GC^T[ACGT]AGC
BsaWI 483 ACCGGA [AT]^CCGG[AT]
BseRI 346 GAGGAG GAGGAG(10/8)
BsiYI 1316 CCGCTGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1316 CCGCTGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 1596 GAGACG CGTCTC(1/5)
BsmFI 1203 GGGAC GGGAC(10/14)
BsmI 1578 GAATGC GAATGC(1/-1)
BspDI 340 ATCGAT AT^CGAT
BspMI 364 GCAGGT ACCTGC(4/8)
BsrDI 130 GCAATG GCAATG(2/0)
BsrFI 1278 GCCGGT [GA]^CCGG[CT]
BstAPI 1255 GCACAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 628 TTCGAA TT^CGAA
BstXI 1287 CCAGTTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 850 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1278 GCCGGT [GA]^CCGG[CT]
ClaI 340 ATCGAT AT^CGAT
DraI 919 TTTAAA TTT^AAA
DraIII 437 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1695 CCATGG C^C[GA][CT]GG
Eam1105I 1134 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1695 CCATGG C^C[AT][AT]GG
HaeIII 1609 GGCC GG^CC
MspA1I 1316 CCGCTG C[AC]G^C[GT]G
NcoI 1695 CCATGG C^CATGG
NspV 628 TTCGAA TT^CGAA
PshBI 331 ATTAAT AT^TAAT
SfcI 600 CTATAG C^T[GA][CT]AG
SfuI 628 TTCGAA TT^CGAA
SspI 1232 AATATT AAT^ATT
StyI 1695 CCATGG C^C[AT][AT]GG
XbaI 510 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 310 CCGC CCGC(-3/-1)
1316 CCGC
AlwNI 200 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1402 CAGATCCTG
AocI 766 CCTGAGG CC^T[ACGT]AGG
1753 CCTGAGG
AspHI 571 GTGCTC G[AT]GC[AT]^C
1253 GTGCAC
BanII 1608 GGGCCC G[GA]GC[CT]^C
1658 GAGCCC
BsaJI 779 CCCAGG C^C[ACGT][ACGT]GG
1695 CCATGG
BsiHKAI 571 GTGCTC G[AT]GC[AT]^C
1253 GTGCAC
BspHI 981 TCATGA T^CATGA
1559 TCATGA
BstYI 1403 AGATCC [GA]^GATC[CT]
1749 GGATCC
Bsu36I 766 CCTGAGG CC^T[ACGT]AGG
1753 CCTGAGG
Cac8I 4 GCAAGC GC[ACGT]^[ACGT]GC
1178 GCTTGC
Eco81I 766 CCTGAGG CC^T[ACGT]AGG
1753 CCTGAGG
HapII 484 CCGG C^CGG
1279 CCGG
HincII 592 GTCAAC GT[CT]^[GA]AC
1282 GTCAAC
HindII 592 GTCAAC GT[CT]^[GA]AC
1282 GTCAAC
HpaII 484 CCGG C^CGG
1279 CCGG
MflI 1403 AGATCC [GA]^GATC[CT]
1749 GGATCC
MspI 484 CCGG C^CGG
1279 CCGG
RcaI 981 TCATGA T^CATGA
1559 TCATGA
SmlI 660 CTTAAG C^T[CT][GA]AG
683 CTCAAG
XcmI 105 CCAAGCCTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
780 CCAGGTTACACCTGG
XhoII 1403 AGATCC [GA]^GATC[CT]
1749 GGATCC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AvaII BbsI BbvI BfaI BmyI
BpuAI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI