Restriction map for
pFN21AE9629
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AviII BseAI
BseRI BsiWI BsmBI BspDI BspEI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
EarI EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MfeI MluI MroI MunI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SexAI SfiI SgrAI SnaBI SpeI
SphI SspI SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 379 GTAGAC GT^[AC][GT]AC
AflII 1999 CTTAAG C^TTAAG
AspHI 1618 GAGCAC G[AT]GC[AT]^C
AvaI 2900 CCCGGG C^[CT]CG[GA]G
AvrII 2640 CCTAGG C^CTAGG
BbrPI 484 CACGTG CAC^GTG
BbsI 49 GAAGAC GAAGAC(2/6)
BbvCI 2028 CCTCAGC CCTCAGC(-5/-2)
BciVI 1440 GTATCC GTATCC(6/5)
BfrI 1999 CTTAAG C^TTAAG
BlnI 2640 CCTAGG C^CTAGG
BlpI 2057 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2057 GCTCAGC GC^T[ACGT]AGC
BpuAI 49 GAAGAC GAAGAC(2/6)
BsaWI 29 TCCGGT [AT]^CCGG[AT]
BsiEI 165 CGGTCG CG[GA][CT]^CG
BsiHKAI 1618 GAGCAC G[AT]GC[AT]^C
BsmI 1184 GCATTC GAATGC(1/-1)
BsoBI 2900 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2138 TGTACA T^GTACA
BspLU11I 2761 ACATGT A^CATGT
BsrFI 2495 GCCGGC [GA]^CCGG[CT]
BsrGI 2138 TGTACA T^GTACA
BstBI 305 TTCGAA TT^CGAA
CelII 2057 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2495 GCCGGC [GA]^CCGG[CT]
DraIII 2131 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2972 GACACACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 650 CCTTTACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 844 GAATTC G^AATTC
EcoRV 1936 GATATC GAT^ATC
HgaI 130 GACGC GACGC(5/10)
NaeI 2495 GCCGGC GCC^GGC
NdeI 1445 CATATG CA^TATG
NgoMIV 2495 GCCGGC G^CCGGC
NheI 2275 GCTAGC G^CTAGC
NspI 2761 ACATGT [GA]CATG^[CT]
NspV 305 TTCGAA TT^CGAA
PmaCI 484 CACGTG CAC^GTG
PmlI 484 CACGTG CAC^GTG
PpuMI 2368 GGGTCCT [GA]G^G[AT]CC[CT]
SbfI 2295 CCTGCAGG CCTGCA^GG
SfuI 305 TTCGAA TT^CGAA
SmaI 2900 CCCGGG CCC^GGG
Sse8387I 2295 CCTGCAGG CCTGCA^GG
SspBI 2138 TGTACA T^GTACA
StuI 1886 AGGCCT AGG^CCT
XbaI 1188 TCTAGA T^CTAGA
XmaCI 2900 CCCGGG C^CCGGG
XmaI 2900 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AcyI 2602 GGCGCC G[GA]^CG[CT]C
2747 GGCGCC
AflIII 485 ACGTGT A^C[GA][CT]GT
2761 ACATGT
AhdI 151 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2917 GACATGGGGTC
ApaI 2262 GGGCCC GGGCC^C
2481 GGGCCC
AspEI 151 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2917 GACATGGGGTC
AspI 1990 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2364 GACTGGGTC
BalI 234 TGGCCA TGG^CCA
2445 TGGCCA
BbeI 2602 GGCGCC GGCGC^C
2747 GGCGCC
BcgI 48 CGAAGACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1563 CGACCTCATTGC
BclI 588 TGATCA T^GATCA
1449 TGATCA
BglI 772 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2618 GCCCACCAGGC
BglII 206 AGATCT A^GATCT
943 AGATCT
BsaAI 484 CACGTG [CT]AC^GT[GA]
1648 TACGTG
BsaHI 2602 GGCGCC G[GA]^CG[CT]C
2747 GGCGCC
BsgI 1828 GTGCAG GTGCAG(16/14)
2722 GTGCAG
BsrDI 1569 CATTGC GCAATG(2/0)
1214 GCAATG
Eam1105I 151 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2917 GACATGGGGTC
FbaI 588 TGATCA T^GATCA
1449 TGATCA
HaeII 2602 GGCGCC [GA]GCGC^[CT]
2747 GGCGCC
Hin1I 2602 GGCGCC G[GA]^CG[CT]C
2747 GGCGCC
KasI 2602 GGCGCC G^GCGCC
2747 GGCGCC
MaeII 485 ACGT A^CGT
1649 ACGT
MluNI 234 TGGCCA TGG^CCA
2445 TGGCCA
MscI 234 TGGCCA TGG^CCA
2445 TGGCCA
NarI 2602 GGCGCC GG^CGCC
2747 GGCGCC
PflFI 1990 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2364 GACTGGGTC
PstI 511 CTGCAG CTGCA^G
2296 CTGCAG
ScaI 359 AGTACT AGT^ACT
1898 AGTACT
SfcI 511 CTGCAG C^T[GA][CT]AG
2296 CTGCAG
SfoI 2602 GGCGCC GGC^GCC
2747 GGCGCC
Tth111I 1990 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2364 GACTGGGTC
XcmI 920 CCATGGCAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1444 CCATATGATCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AvaII
BamHI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaBI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII