Restriction map for
pFN21AB5882
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI Alw44I ApaLI AseI
AsnI Asp700I Asp718I AspI AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BsaI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
EarI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI Ksp632I MaeI
MamI MfeI MluI MluNI MscI
MseI MunI NcoI NdeI NheI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 966 TCCGGA T^CCGGA
AcsI 943 GAATTC [GA]^AATT[CT]
AhdI 372 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 667 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1081 CCTCAGG CC^T[ACGT]AGG
ApoI 943 GAATTC [GA]^AATT[CT]
AscI 406 GGCGCGCC GG^CGCGCC
AspEI 372 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1280 TGCGCA TGC^GCA
BsaAI 525 CACGTA [CT]AC^GT[GA]
BseAI 966 TCCGGA T^CCGGA
BsgI 993 GTGCAG GTGCAG(16/14)
BsmBI 364 GAGACG CGTCTC(1/5)
BspEI 966 TCCGGA T^CCGGA
BstAPI 666 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 369 GGTGACC G^GT[ACGT]ACC
BstPI 369 GGTGACC G^GT[ACGT]ACC
Bsu36I 1081 CCTCAGG CC^T[ACGT]AGG
DraIII 709 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1051 GACCAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 372 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1081 CCTCAGG CC^T[ACGT]AGG
EcoNI 1077 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 369 GGTGACC G^GT[ACGT]ACC
EcoRI 943 GAATTC G^AATTC
EcoT14I 1406 CCAAGG C^C[AT][AT]GG
FspI 1280 TGCGCA TGC^GCA
HincII 1234 GTCAAC GT[CT]^[GA]AC
HindII 1234 GTCAAC GT[CT]^[GA]AC
MroI 966 TCCGGA T^CCGGA
MslI 1305 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 959 GCATGT [GA]CATG^[CT]
PflMI 1406 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 667 CAGCTG CAG^CTG
SacI 303 GAGCTC GAGCT^C
SfiI 435 GGCCGGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 547 CTCAAG C^T[CT][GA]AG
StyI 1406 CCAAGG C^C[AT][AT]GG
Tsp509I 944 AATT ^AATT
Van91I 1406 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 422 CCAGCGACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AlwI 1467 GATCC GGATC(4/5)
873 GGATC
Aor51HI 554 AGCGCT AGC^GCT
1325 AGCGCT
AvaII 8 GGTCC G^G[AT]CC
54 GGTCC
BbeI 226 GGCGCC GGCGC^C
245 GGCGCC
BcgI 1151 GCAGCAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1394 GCATCAGCTTCG
BlpI 127 GCTGAGC GC^T[ACGT]AGC
1176 GCTCAGC
Bpu1102I 127 GCTGAGC GC^T[ACGT]AGC
1176 GCTCAGC
BsaWI 966 TCCGGA [AT]^CCGG[AT]
1016 ACCGGA
BseRI 848 CTCCTC GAGGAG(10/8)
1471 CTCCTC
BspMI 373 ACCTGC ACCTGC(4/8)
701 ACCTGC
BsrBI 168 CCGCTC CCGCTC(-3/-3)
391 GAGCGG
BstF5I 1170 CATCC GGATG(2/0)
105 GGATG
BstYI 873 GGATCT [GA]^GATC[CT]
1466 AGATCC
CelII 127 GCTGAGC GC^T[ACGT]AGC
1176 GCTCAGC
Eco47III 554 AGCGCT AGC^GCT
1325 AGCGCT
FokI 1170 CATCC GGATG(9/13)
105 GGATG
KasI 226 GGCGCC G^GCGCC
245 GGCGCC
MaeII 317 ACGT A^CGT
526 ACGT
MboII 1067 TCTTC GAAGA(8/7)
1229 TCTTC
MflI 873 GGATCT [GA]^GATC[CT]
1466 AGATCC
NarI 226 GGCGCC GG^CGCC
245 GGCGCC
NlaIII 276 CATG CATG^
960 CATG
NotI 164 GCGGCCGC GC^GGCCGC
1262 GCGGCCGC
NruI 474 TCGCGA TCG^CGA
852 TCGCGA
PstI 703 CTGCAG CTGCA^G
1336 CTGCAG
SfcI 703 CTGCAG C^T[GA][CT]AG
1336 CTGCAG
SfoI 226 GGCGCC GGC^GCC
245 GGCGCC
SgrAI 247 CGCCGGCG C[GA]^CCGG[CT]G
402 CGCCGGCG
TaqI 1316 TCGA T^CGA
1322 TCGA
TfiI 817 GAATC G^A[AT]TC
883 GATTC
TthHB8I 1316 TCGA T^CGA
1322 TCGA
XhoII 873 GGATCT [GA]^GATC[CT]
1466 AGATCC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AspHI AvaI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KspI MaeIII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIV PleI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SmaI
TseI Tsp45I TspRI XmaCI XmaI