Restriction map for
pFN21AB6296
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BspMI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoT22I FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KspI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 241 GGTACC G^GTACC
AlwI 1041 GGATC GGATC(4/5)
Asp718I 241 GGTACC G^GTACC
BanII 2036 GAGCTC G[GA]GC[CT]^C
BfaI 895 CTAG C^TAG
BglI 2134 GCCTACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1923 AGATCT A^GATCT
BsaBI 102 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 145 CTGCAC GTGCAG(16/14)
BsmI 533 GCATTC GAATGC(1/-1)
Bsp1407I 770 TGTACA T^GTACA
BsrBI 80 GAGCGG CCGCTC(-3/-3)
BsrDI 290 GCAATG GCAATG(2/0)
BsrGI 770 TGTACA T^GTACA
BstEII 1569 GGTAACC G^GT[ACGT]ACC
BstPI 1569 GGTAACC G^GT[ACGT]ACC
CfoI 62 GCGC GCG^C
DsaI 222 CCATGG C^C[GA][CT]GG
EarI 995 GAAGAG CTCTTC(1/4)
EcoO65I 1569 GGTAACC G^GT[ACGT]ACC
EcoT14I 222 CCATGG C^C[AT][AT]GG
HhaI 62 GCGC GCG^C
HinP1I 62 GCGC G^CGC
HincII 2177 GTCAAC GT[CT]^[GA]AC
HindII 2177 GTCAAC GT[CT]^[GA]AC
KpnI 241 GGTACC GGTAC^C
Ksp632I 995 GAAGAG CTCTTC(1/4)
MaeI 895 CTAG C^TAG
MaeII 149 ACGT A^CGT
MamI 102 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 222 CCATGG C^CATGG
NdeI 1676 CATATG CA^TATG
NspI 1716 ACATGC [GA]CATG^[CT]
PflMI 940 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2045 CTGCAG CTGCA^G
SacI 2036 GAGCTC GAGCT^C
ScaI 1543 AGTACT AGT^ACT
SspBI 770 TGTACA T^GTACA
StuI 2272 AGGCCT AGG^CCT
StyI 222 CCATGG C^C[AT][AT]GG
SwaI 476 ATTTAAAT ATTT^AAAT
TaqI 975 TCGA T^CGA
Tsp45I 541 GTGAC ^GT[GC]AC
TthHB8I 975 TCGA T^CGA
Van91I 940 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 151 GTCTAC GT^[AC][GT]AC
1140 GTCTAC
AccII 61 CGCG CG^CG
135 CGCG
AflII 1912 CTTAAG C^TTAAG
2209 CTTAAG
AspHI 647 GTGCTC G[AT]GC[AT]^C
2036 GAGCTC
BanI 241 GGTACC G^G[CT][GA]CC
1074 GGCACC
BclI 104 TGATCA T^GATCA
1094 TGATCA
BcnI 65 CCCGG CC^[GC]GG
93 CCGGG
BfrI 1912 CTTAAG C^TTAAG
2209 CTTAAG
BmyI 647 GTGCTC G[AGT]GC[ACT]^C
2036 GAGCTC
BseRI 16 CTCCTC GAGGAG(10/8)
194 GAGGAG
BsiHKAI 647 GTGCTC G[AT]GC[AT]^C
2036 GAGCTC
Bsp1286I 647 GTGCTC G[AGT]GC[ACT]^C
2036 GAGCTC
BspHI 825 TCATGA T^CATGA
2189 TCATGA
BsrFI 51 GCCGGT [GA]^CCGG[CT]
1071 ACCGGC
BstAPI 1405 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1681 GCACTGCCTGC
BstUI 61 CGCG CG^CG
135 CGCG
BstYI 1041 GGATCT [GA]^GATC[CT]
1923 AGATCT
Cfr10I 51 GCCGGT [GA]^CCGG[CT]
1071 ACCGGC
EcoRV 199 GATATC GAT^ATC
2292 GATATC
FauI 59 CCCGC CCCGC(4/6)
1777 GCGGG
FbaI 104 TGATCA T^GATCA
1094 TGATCA
MflI 1041 GGATCT [GA]^GATC[CT]
1923 AGATCT
MslI 534 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
826 CATGATTGTG
MvnI 61 CGCG CG^CG
135 CGCG
NciI 65 CCCGG CC^[GC]GG
93 CCGGG
PleI 1746 GACTC GAGTC(4/5)
1232 GAGTC
RcaI 825 TCATGA T^CATGA
2189 TCATGA
XhoII 1041 GGATCT [GA]^GATC[CT]
1923 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BalI BbvI BciVI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TfiI Tru9I TseI Tsp509I TspRI