Restriction map for
pFN21AE0662
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbeI BbrPI BbsI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspMI BsrDI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV FseI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MfeI MluI
MroI MseI MslI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SfcI
SfiI SfoI SfuI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 154 GACGTC GACGT^C
AflIII 635 ACATGT A^C[GA][CT]GT
AgeI 160 ACCGGT A^CCGGT
Alw44I 439 GTGCAC G^TGCAC
AlwNI 703 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 439 GTGCAC G^TGCAC
AviII 233 TGCGCA TGC^GCA
BalI 1065 TGGCCA TGG^CCA
BbvCI 714 GCTGAGG CCTCAGC(-5/-2)
BcgI 544 CGAGAGGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 722 TGATCA T^GATCA
BglI 684 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 993 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 659 TGTACA T^GTACA
BspHI 725 TCATGA T^CATGA
BspLU11I 635 ACATGT A^CATGT
BsrBI 729 GAGCGG CCGCTC(-3/-3)
BsrGI 659 TGTACA T^GTACA
BssSI 123 CACGAG CACGAG(-5/-1)
BstEII 357 GGTCACC G^GT[ACGT]ACC
BstPI 357 GGTCACC G^GT[ACGT]ACC
BstXI 563 CCAGTTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1069 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 357 GGTCACC G^GT[ACGT]ACC
EcoT22I 108 ATGCAT ATGCA^T
FbaI 722 TGATCA T^GATCA
FspI 233 TGCGCA TGC^GCA
MaeII 155 ACGT A^CGT
MamI 993 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1065 TGGCCA TGG^CCA
MscI 1065 TGGCCA TGG^CCA
NsiI 108 ATGCAT ATGCA^T
NspI 635 ACATGT [GA]CATG^[CT]
PinAI 160 ACCGGT A^CCGGT
PleI 25 GACTC GAGTC(4/5)
RcaI 725 TCATGA T^CATGA
ScaI 869 AGTACT AGT^ACT
SexAI 788 ACCTGGT A^CC[AT]GGT
SgrAI 159 CACCGGTG C[GA]^CCGG[CT]G
SmlI 363 CTTGAG C^T[CT][GA]AG
SspBI 659 TGTACA T^GTACA
StuI 842 AGGCCT AGG^CCT
Tsp509I 55 AATT ^AATT
XcmI 65 CCAAAGAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwI 72 GATCC GGATC(4/5)
950 GGATC
BanI 248 GGCACC G^G[CT][GA]CC
692 GGCACC
BpmI 403 CTGGAG CTGGAG(16/14)
523 CTGGAG
BsaWI 160 ACCGGT [AT]^CCGG[AT]
695 ACCGGA
BsgI 35 CTGCAC GTGCAG(16/14)
414 CTGCAC
BstYI 71 AGATCC [GA]^GATC[CT]
950 GGATCT
CfoI 234 GCGC GCG^C
1029 GCGC
EarI 321 GAAGAG CTCTTC(1/4)
838 GAAGAG
EcoNI 231 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1143 CCTGGCCGAGG
EcoT14I 845 CCTTGG C^C[AT][AT]GG
1124 CCAAGG
HgaI 1042 GACGC GACGC(5/10)
650 GCGTC
HhaI 234 GCGC GCG^C
1029 GCGC
HinP1I 234 GCGC G^CGC
1029 GCGC
Ksp632I 321 GAAGAG CTCTTC(1/4)
838 GAAGAG
MflI 71 AGATCC [GA]^GATC[CT]
950 GGATCT
NaeI 673 GCCGGC GCC^GGC
1031 GCCGGC
NgoMIV 673 GCCGGC G^CCGGC
1031 GCCGGC
PvuII 265 CAGCTG CAG^CTG
520 CAGCTG
SacI 331 GAGCTC GAGCT^C
395 GAGCTC
StyI 845 CCTTGG C^C[AT][AT]GG
1124 CCAAGG
TfiI 800 GATTC G^A[AT]TC
1058 GAATC
XhoII 71 AGATCC [GA]^GATC[CT]
950 GGATCT
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII Hin1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
TaqI TseI Tsp45I TspRI TthHB8I
XmaCI XmaI