Restriction map for
pFN21AE4062
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbsI
BbvCI BciVI BcnI BfrI BglI
BglII BlnI BpuAI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SspBI SspI
StyI SwaI TspRI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 94 AAATTC [GA]^AATT[CT]
AfaI 372 GTAC GT^AC
ApoI 94 AAATTC [GA]^AATT[CT]
AvaII 543 GGACC G^G[AT]CC
BalI 500 TGGCCA TGG^CCA
BanII 853 GAGCTC G[GA]GC[CT]^C
BbrPI 260 CACGTG CAC^GTG
BcgI 804 CGACCTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 530 TGATCA T^GATCA
BpmI 654 CTGGAG CTGGAG(16/14)
BsaAI 260 CACGTG [CT]AC^GT[GA]
BsaI 433 GAGACC GGTCTC(1/5)
BsaWI 227 ACCGGA [AT]^CCGG[AT]
BseRI 159 GAGGAG GAGGAG(10/8)
BsmFI 594 GTCCC GGGAC(10/14)
BsrBI 136 GAGCGG CCGCTC(-3/-3)
BsrDI 255 CATTGC GCAATG(2/0)
BsrI 450 CCAGT ACTGG(1/-1)
BssSI 430 CACGAG CACGAG(-5/-1)
Cfr13I 543 GGACC G^G[ACGT]CC
EaeI 500 TGGCCA [CT]^GGCC[GA]
EcoNI 71 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 530 TGATCA T^GATCA
HapII 228 CCGG C^CGG
HpaII 228 CCGG C^CGG
HphI 533 TCACC GGTGA(8/7)
MluNI 500 TGGCCA TGG^CCA
MscI 500 TGGCCA TGG^CCA
MseI 883 TTAA T^TAA
MspI 228 CCGG C^CGG
NlaIV 543 GGACCC GG[ACGT]^[ACGT]CC
PleI 243 GACTC GAGTC(4/5)
PmaCI 260 CACGTG CAC^GTG
PmlI 260 CACGTG CAC^GTG
RsaI 372 GTAC GT^AC
SacI 853 GAGCTC GAGCT^C
SapI 402 GCTCTTC GCTCTTC(1/4)
Sau96I 543 GGACC G^G[ACGT]CC
SbfI 689 CCTGCAGG CCTGCA^GG
SphI 722 GCATGC GCATG^C
Sse8387I 689 CCTGCAGG CCTGCA^GG
StuI 176 AGGCCT AGG^CCT
TfiI 201 GATTC G^A[AT]TC
Tru9I 883 TTAA T^TAA
Tsp45I 263 GTGAC ^GT[GC]AC
Tsp509I 95 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 289 GATCC GGATC(4/5)
333 GATCC
BbvI 726 GCTGC GCAGC(8/12)
750 GCTGC
BfaI 325 CTAG C^TAG
511 CTAG
BsmAI 241 GAGAC GTCTC(1/5)
433 GAGAC
BspMI 221 ACCTGC ACCTGC(4/8)
744 GCAGGT
BstAPI 729 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
744 GCAGGTGCTGC
BstYI 288 AGATCC [GA]^GATC[CT]
332 AGATCC
FauI 738 GCGGG CCCGC(4/6)
767 GCGGG
HinfI 201 GATTC G^A[ACGT]TC
243 GACTC
MaeI 325 CTAG C^TAG
511 CTAG
MaeIII 263 GTGAC ^GT[ACGT]AC
347 GTTAC
MflI 288 AGATCC [GA]^GATC[CT]
332 AGATCC
MslI 475 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
492 CATTGGCGTG
MspA1I 409 CAGCTG C[AC]G^C[GT]G
477 CAGCTG
NspI 515 ACATGC [GA]CATG^[CT]
722 GCATGC
PflMI 408 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
702 CCAGGTGCTGG
PvuII 409 CAGCTG CAG^CTG
477 CAGCTG
SfaNI 399 GATGC GCATC(5/9)
811 GATGC
TseI 726 GCTGC G^C[AT]GC
750 GCTGC
Van91I 408 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
702 CCAGGTGCTGG
XhoII 288 AGATCC [GA]^GATC[CT]
332 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AlwNI AspHI BlpI
BmyI Bpu1102I BsaJI BsgI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I CelII DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
ItaI Ksp632I MaeII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
PstI Sau3AI ScrFI SfcI TaqI
TthHB8I