Restriction map for
pFN21AE4057
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstYI BstZ17I ClaI DraI
DraIII Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MaeI MfeI MflI MluI MluNI
MroI MscI MseI MunI NarI
NcoI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 6 GACGCC G[GA]^CG[CT]C
AfaI 429 GTAC GT^AC
AlwI 360 GATCC GGATC(4/5)
AlwNI 139 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 757 CCTGAGG CC^T[ACGT]AGG
ApaI 256 GGGCCC GGGCC^C
Asp700I 159 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 840 GGCACC G^G[CT][GA]CC
BglI 34 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 360 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 6 GACGCC G[GA]^CG[CT]C
BsaWI 377 ACCGGA [AT]^CCGG[AT]
BsgI 703 GTGCAG GTGCAG(16/14)
BspMI 705 GCAGGT ACCTGC(4/8)
BstEII 402 GGTCACC G^GT[ACGT]ACC
BstPI 402 GGTCACC G^GT[ACGT]ACC
BstXI 870 CCAGGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 757 CCTGAGG CC^T[ACGT]AGG
CpoI 203 CGGACCG CG^G[AT]CCG
DrdI 394 GACTACACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 10 CCGCGG C^C[GA][CT]GG
Eco81I 757 CCTGAGG CC^T[ACGT]AGG
EcoNI 177 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 402 GGTCACC G^GT[ACGT]ACC
EcoT14I 575 CCAAGG C^C[AT][AT]GG
Hin1I 6 GACGCC G[GA]^CG[CT]C
HinfI 613 GAGTC G^A[ACGT]TC
KspI 10 CCGCGG CCGC^GG
MaeII 107 ACGT A^CGT
MamI 360 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 65 GCGGCCGC GC^GGCCGC
NspI 423 GCATGT [GA]CATG^[CT]
PleI 613 GAGTC GAGTC(4/5)
RsaI 429 GTAC GT^AC
RsrII 203 CGGACCG CG^G[AT]CCG
SacII 10 CCGCGG CCGC^GG
SapI 190 GAAGAGC GCTCTTC(1/4)
SgrAI 50 CGCCGGCG C[GA]^CCGG[CT]G
StyI 575 CCAAGG C^C[AT][AT]GG
TaqI 500 TCGA T^CGA
TthHB8I 500 TCGA T^CGA
XmnI 159 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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BbvCI 168 CCTCAGC CCTCAGC(-5/-2)
310 GCTGAGG
BlpI 603 GCTGAGC GC^T[ACGT]AGC
763 GCTGAGC
Bpu1102I 603 GCTGAGC GC^T[ACGT]AGC
763 GCTGAGC
BsaI 214 GAGACC GGTCTC(1/5)
471 GGTCTC
BsiEI 13 CGGCCG CG[GA][CT]^CG
66 CGGCCG
BsmAI 214 GAGAC GTCTC(1/5)
472 GTCTC
BsmFI 873 GGGAC GGGAC(10/14)
412 GTCCC
BsrFI 15 GCCGGC [GA]^CCGG[CT]
51 GCCGGC
CelII 603 GCTGAGC GC^T[ACGT]AGC
763 GCTGAGC
Cfr10I 15 GCCGGC [GA]^CCGG[CT]
51 GCCGGC
DpnII 360 GATC ^GATC
862 GATC
EaeI 13 CGGCCG [CT]^GGCC[GA]
66 CGGCCG
EagI 13 CGGCCG C^GGCCG
66 CGGCCG
EarI 190 GAAGAG CTCTTC(1/4)
385 GAAGAG
EclXI 13 CGGCCG C^GGCCG
66 CGGCCG
Eco52I 13 CGGCCG C^GGCCG
66 CGGCCG
HaeII 45 AGCGCC [GA]GCGC^[CT]
494 GGCGCT
HgaI 6 GACGC GACGC(5/10)
141 GACGC
Ksp632I 190 GAAGAG CTCTTC(1/4)
385 GAAGAG
MboI 360 GATC ^GATC
862 GATC
MboII 190 GAAGA GAAGA(8/7)
385 GAAGA
MslI 462 CACGCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
842 CACCCGGATG
NaeI 15 GCCGGC GCC^GGC
51 GCCGGC
NdeII 360 GATC ^GATC
862 GATC
NgoMIV 15 GCCGGC G^CCGGC
51 GCCGGC
PpuMI 320 GGGTCCT [GA]G^G[AT]CC[CT]
410 AGGTCCC
PstI 196 CTGCAG CTGCA^G
439 CTGCAG
PvuII 505 CAGCTG CAG^CTG
646 CAGCTG
Sau3AI 360 GATC ^GATC
862 GATC
SfaNI 718 GATGC GCATC(5/9)
365 GCATC
SfcI 196 CTGCAG C^T[GA][CT]AG
439 CTGCAG
Tsp45I 239 GTGAC ^GT[GC]AC
403 GTCAC
TspRI 442 CAGTG CA[GC]TG[ACGT][ACGT]^
773 CAGTG
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV SacI Sau96I ScrFI
TseI