Restriction map for
pFN21AE3473
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# NO CUT ENZYMES ( 70 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbsI BciVI BclI BfrI
BlnI BpuAI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eco47III EcoRI EcoRV EcoT22I
FbaI FspI HpaI KpnI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshBI Psp1406I PvuI
RsrII ScaI SfuI SnaBI SpeI
SspBI SspI SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AflIII 4526 ACGTGT A^C[GA][CT]GT
AgeI 4382 ACCGGT A^CCGGT
Alw44I 4522 GTGCAC G^TGCAC
ApaLI 4522 GTGCAC G^TGCAC
AspI 1317 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 4525 CACGTG CAC^GTG
BcgI 3240 GCAGGACAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 4525 CACGTG [CT]AC^GT[GA]
BsaBI 82 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4382 ACCGGT [AT]^CCGG[AT]
BsmBI 1166 CGTCTC CGTCTC(1/5)
BspHI 102 TCATGA T^CATGA
BssSI 702 CACGAG CACGAG(-5/-1)
BstEII 354 GGTGACC G^GT[ACGT]ACC
BstPI 354 GGTGACC G^GT[ACGT]ACC
EcoO65I 354 GGTGACC G^GT[ACGT]ACC
HincII 4252 GTCGAC GT[CT]^[GA]AC
HindII 4252 GTCGAC GT[CT]^[GA]AC
MamI 82 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 178 TTAA T^TAA
PflFI 1317 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4382 ACCGGT A^CCGGT
PmaCI 4525 CACGTG CAC^GTG
PmlI 4525 CACGTG CAC^GTG
PshAI 23 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 102 TCATGA T^CATGA
SalI 4252 GTCGAC G^TCGAC
SexAI 425 ACCTGGT A^CC[AT]GGT
SfiI 4227 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 3693 CGCCGGCG C[GA]^CCGG[CT]G
StuI 4515 AGGCCT AGG^CCT
Tru9I 178 TTAA T^TAA
Tth111I 1317 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1461 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AhdI 1999 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2725 GACCCCTCGTC
AocI 739 CCTGAGG CC^T[ACGT]AGG
2479 CCTCAGG
AspEI 1999 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2725 GACCCCTCGTC
BglII 1709 AGATCT A^GATCT
1886 AGATCT
BsmI 2400 GCATTC GAATGC(1/-1)
2645 GCATTC
BssHII 1769 GCGCGC G^CGCGC
2556 GCGCGC
Bsu36I 739 CCTGAGG CC^T[ACGT]AGG
2479 CCTCAGG
DraIII 349 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2365 CACACGGTG
DrdI 4243 GACCGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4377 GACGCACCGGTC
Eam1105I 1999 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2725 GACCCCTCGTC
Eco81I 739 CCTGAGG CC^T[ACGT]AGG
2479 CCTCAGG
HindIII 198 AAGCTT A^AGCTT
1456 AAGCTT
NspI 209 GCATGC [GA]CATG^[CT]
636 GCATGC
PpuMI 2483 AGGACCC [GA]G^G[AT]CC[CT]
2935 GGGTCCC
SbfI 1530 CCTGCAGG CCTGCA^GG
4440 CCTGCAGG
SmaI 3921 CCCGGG CCC^GGG
4238 CCCGGG
SphI 209 GCATGC GCATG^C
636 GCATGC
Sse8387I 1530 CCTGCAGG CCTGCA^GG
4440 CCTGCAGG
XmaCI 3921 CCCGGG C^CCGGG
4238 CCCGGG
XmaI 3921 CCCGGG C^CCGGG
4238 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FseI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PstI PvuII RsaI SacI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII