Restriction map for
pFN21AB6251
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccI AclI AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BcgI BcnI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KspI MaeII
MamI MluI MluNI MscI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 940 GGTACC G^GTACC
AccII 523 CGCG CG^CG
AccIII 713 TCCGGA T^CCGGA
AcyI 1813 GACGCC G[GA]^CG[CT]C
AflII 514 CTTAAG C^TTAAG
AhdI 1575 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1044 CCTCAGG CC^T[ACGT]AGG
Asp700I 856 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 940 GGTACC G^GTACC
AspEI 1575 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 892 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 709 GAGCTC G[GA]GC[CT]^C
BbvCI 1102 GCTGAGG CCTCAGC(-5/-2)
BfrI 514 CTTAAG C^TTAAG
BsaHI 1813 GACGCC G[GA]^CG[CT]C
BsaWI 713 TCCGGA [AT]^CCGG[AT]
BseAI 713 TCCGGA T^CCGGA
BsiYI 1157 CCATCAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1157 CCATCAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspEI 713 TCCGGA T^CCGGA
BspHI 864 TCATGA T^CATGA
BsrBI 945 CCGCTC CCGCTC(-3/-3)
BsrFI 611 ACCGGC [GA]^CCGG[CT]
BstUI 523 CGCG CG^CG
Bsu36I 1044 CCTCAGG CC^T[ACGT]AGG
CfoI 754 GCGC GCG^C
Cfr10I 611 ACCGGC [GA]^CCGG[CT]
DraIII 902 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1370 CCATGG C^C[GA][CT]GG
Eam1105I 1575 GACTGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1044 CCTCAGG CC^T[ACGT]AGG
HaeII 753 GGCGCT [GA]GCGC^[CT]
HgaI 1813 GACGC GACGC(5/10)
HhaI 754 GCGC GCG^C
Hin1I 1813 GACGCC G[GA]^CG[CT]C
HinP1I 754 GCGC G^CGC
KpnI 940 GGTACC GGTAC^C
MfeI 2069 CAATTG C^AATTG
MroI 713 TCCGGA T^CCGGA
MunI 2069 CAATTG C^AATTG
MvnI 523 CGCG CG^CG
NcoI 1370 CCATGG C^CATGG
PflFI 892 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 864 TCATGA T^CATGA
SacI 709 GAGCTC GAGCT^C
SspI 499 AATATT AAT^ATT
StuI 1557 AGGCCT AGG^CCT
Tth111I 892 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 856 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 1844 AAATTC [GA]^AATT[CT]
1900 AAATTC
AfaI 941 GTAC GT^AC
1007 GTAC
AlwNI 1271 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
1601 CAGCTGCTG
ApoI 1844 AAATTC [GA]^AATT[CT]
1900 AAATTC
AspHI 3 GAGCAC G[AT]GC[AT]^C
709 GAGCTC
BciVI 804 GGATAC GTATCC(6/5)
1894 GTATCC
BmyI 3 GAGCAC G[AGT]GC[ACT]^C
709 GAGCTC
BsgI 1309 GTGCAG GTGCAG(16/14)
1687 GTGCAG
BsiHKAI 3 GAGCAC G[AT]GC[AT]^C
709 GAGCTC
BsmAI 846 GAGAC GTCTC(1/5)
2037 GTCTC
Bsp1286I 3 GAGCAC G[AGT]GC[ACT]^C
709 GAGCTC
Cac8I 520 GCACGC GC[ACGT]^[ACGT]GC
1333 GCCCGC
FauI 944 CCCGC CCCGC(4/6)
1334 CCCGC
HaeIII 614 GGCC GG^CC
1558 GGCC
HapII 612 CCGG C^CGG
714 CCGG
HincII 870 GTTGAC GT[CT]^[GA]AC
1435 GTTGAC
HindII 870 GTTGAC GT[CT]^[GA]AC
1435 GTTGAC
HpaII 612 CCGG C^CGG
714 CCGG
MspA1I 920 CAGCTG C[AC]G^C[GT]G
1601 CAGCTG
MspI 612 CCGG C^CGG
714 CCGG
NspI 647 GCATGT [GA]CATG^[CT]
1314 GCATGT
PvuII 920 CAGCTG CAG^CTG
1601 CAGCTG
RsaI 941 GTAC GT^AC
1007 GTAC
TaqI 1772 TCGA T^CGA
2258 TCGA
TthHB8I 1772 TCGA T^CGA
2258 TCGA
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbvI BclI BfaI BpmI BsaJI
BsmFI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I FbaI Fnu4HI
FokI HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII