Restriction map for
pFN21AB8513
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI AspEI AviII AvrII
BbrPI BcgI BfrI BlnI BsaAI
BsiWI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DraI
DraIII Eam1105I EcoT22I FseI FspI
HindIII HpaI KspI MfeI MunI
NdeI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SbfI ScaI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 2728 GACGTC GACGT^C
Acc65I 340 GGTACC G^GTACC
AccI 2788 GTCGAC GT^[AC][GT]AC
AccIII 1623 TCCGGA T^CCGGA
AcsI 1397 GAATTC [GA]^AATT[CT]
Alw44I 1954 GTGCAC G^TGCAC
ApaLI 1954 GTGCAC G^TGCAC
ApoI 1397 GAATTC [GA]^AATT[CT]
Asp700I 1397 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 340 GGTACC G^GTACC
AspI 594 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 896 TGGCCA TGG^CCA
BciVI 770 GTATCC GTATCC(6/5)
BclI 35 TGATCA T^GATCA
BglII 1449 AGATCT A^GATCT
BlpI 123 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC
BsaBI 2226 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1623 TCCGGA [AT]^CCGG[AT]
BseAI 1623 TCCGGA T^CCGGA
BspEI 1623 TCCGGA T^CCGGA
BspHI 38 TCATGA T^CATGA
BspLU11I 2294 ACATGT A^CATGT
BsrDI 612 GCAATG GCAATG(2/0)
BssSI 2611 CACGAG CACGAG(-5/-1)
BstAPI 405 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2573 TTCGAA TT^CGAA
BstEII 2496 GGTCACC G^GT[ACGT]ACC
BstPI 2496 GGTCACC G^GT[ACGT]ACC
CelII 123 GCTGAGC GC^T[ACGT]AGC
DrdI 2799 GACCGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2496 GGTCACC G^GT[ACGT]ACC
EcoRI 1397 GAATTC G^AATTC
EcoRV 1115 GATATC GAT^ATC
FbaI 35 TGATCA T^GATCA
HincII 2788 GTCGAC GT[CT]^[GA]AC
HindII 2788 GTCGAC GT[CT]^[GA]AC
KpnI 340 GGTACC GGTAC^C
MamI 2226 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1552 ACGCGT A^CGCGT
MluNI 896 TGGCCA TGG^CCA
MroI 1623 TCCGGA T^CCGGA
MscI 896 TGGCCA TGG^CCA
MseI 889 TTAA T^TAA
NaeI 233 GCCGGC GCC^GGC
NgoMIV 233 GCCGGC G^CCGGC
NheI 883 GCTAGC G^CTAGC
NspV 2573 TTCGAA TT^CGAA
PflFI 594 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 38 TCATGA T^CATGA
SalI 2788 GTCGAC G^TCGAC
SapI 60 GAAGAGC GCTCTTC(1/4)
SfuI 2573 TTCGAA TT^CGAA
SmlI 988 CTTGAG C^T[CT][GA]AG
SphI 1130 GCATGC GCATG^C
Tru9I 889 TTAA T^TAA
Tth111I 594 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2583 TCTAGA T^CTAGA
XmnI 1397 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1552 ACGCGT A^C[GA][CT]GT
2294 ACATGT
AlwNI 1033 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2713 CAGACCCTG
Aor51HI 2081 AGCGCT AGC^GCT
2162 AGCGCT
BamHI 2000 GGATCC G^GATCC
2051 GGATCC
BbeI 157 GGCGCC GGCGC^C
571 GGCGCC
BbvCI 1361 CCTCAGC CCTCAGC(-5/-2)
2112 GCTGAGG
BsaI 2392 GAGACC GGTCTC(1/5)
1961 GGTCTC
BsgI 412 GTGCAG GTGCAG(16/14)
1492 GTGCAG
BsiEI 231 CGGCCG CG[GA][CT]^CG
705 CGGCCG
BsmI 689 GAATGC GAATGC(1/-1)
1412 GAATGC
BsrBI 63 GAGCGG CCGCTC(-3/-3)
228 GAGCGG
EagI 231 CGGCCG C^GGCCG
705 CGGCCG
EclXI 231 CGGCCG C^GGCCG
705 CGGCCG
Eco47III 2081 AGCGCT AGC^GCT
2162 AGCGCT
Eco52I 231 CGGCCG C^GGCCG
705 CGGCCG
KasI 157 GGCGCC G^GCGCC
571 GGCGCC
MaeII 410 ACGT A^CGT
2729 ACGT
NarI 157 GGCGCC GG^CGCC
571 GGCGCC
NcoI 1526 CCATGG C^CATGG
2136 CCATGG
PvuII 2005 CAGCTG CAG^CTG
2036 CAGCTG
SacI 462 GAGCTC GAGCT^C
2254 GAGCTC
SfoI 157 GGCGCC GGC^GCC
571 GGCGCC
StuI 1667 AGGCCT AGG^CCT
2504 AGGCCT
TfiI 2231 GAATC G^A[AT]TC
2435 GAATC
Tsp509I 813 AATT ^AATT
1398 AATT
XcmI 2405 CCACGGCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2610 CCACGAGGTCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XhoII XmaCI XmaI