Restriction map for
pFN21AB6280
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AclI AflII AflIII Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BstAPI BstBI Bsu36I ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoT22I FbaI HincII HindII HpaI
KasI MaeI MamI MfeI MluI
MunI NarI NcoI NheI NotI
NruI NsiI NspI NspV PacI
PflMI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 1062 GGTACC G^GTACC
AccIII 1465 TCCGGA T^CCGGA
AcyI 169 GACGCC G[GA]^CG[CT]C
AgeI 1935 ACCGGT A^CCGGT
AhdI 2020 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 801 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1062 GGTACC G^GTACC
AspEI 2020 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 629 TGCGCA TGC^GCA
BalI 767 TGGCCA TGG^CCA
BbvCI 963 CCTCAGC CCTCAGC(-5/-2)
BglI 229 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 126 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 126 GCTGAGC GC^T[ACGT]AGC
BsaAI 425 TACGTG [CT]AC^GT[GA]
BsaHI 169 GACGCC G[GA]^CG[CT]C
BsaI 847 GAGACC GGTCTC(1/5)
BseAI 1465 TCCGGA T^CCGGA
BsmBI 673 GAGACG CGTCTC(1/5)
BspEI 1465 TCCGGA T^CCGGA
BssHII 565 GCGCGC G^CGCGC
BssSI 460 CTCGTG CACGAG(-5/-1)
Bst1107I 1660 GTATAC GTA^TAC
BstEII 2089 GGTCACC G^GT[ACGT]ACC
BstPI 2089 GGTCACC G^GT[ACGT]ACC
BstZ17I 1660 GTATAC GTA^TAC
CelII 126 GCTGAGC GC^T[ACGT]AGC
DraII 1113 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 591 CCGCGG C^C[GA][CT]GG
Eam1105I 2020 GACCTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1113 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 2089 GGTCACC G^GT[ACGT]ACC
EcoRI 1258 GAATTC G^AATTC
EcoRV 1020 GATATC GAT^ATC
EcoT14I 2052 CCTTGG C^C[AT][AT]GG
FseI 595 GGCCGGCC GGCCGG^CC
FspI 629 TGCGCA TGC^GCA
HgaI 169 GACGC GACGC(5/10)
Hin1I 169 GACGCC G[GA]^CG[CT]C
HindIII 1056 AAGCTT A^AGCTT
KpnI 1062 GGTACC GGTAC^C
KspI 591 CCGCGG CCGC^GG
MluNI 767 TGGCCA TGG^CCA
MroI 1465 TCCGGA T^CCGGA
MscI 767 TGGCCA TGG^CCA
MslI 1350 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 979 CATATG CA^TATG
PaeR7I 505 CTCGAG C^TCGAG
PinAI 1935 ACCGGT A^CCGGT
PpuMI 1113 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1959 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 591 CCGCGG CCGC^GG
SapI 1031 GCTCTTC GCTCTTC(1/4)
ScaI 1072 AGTACT AGT^ACT
SgrAI 1934 CACCGGTG C[GA]^CCGG[CT]G
StuI 1335 AGGCCT AGG^CCT
StyI 2052 CCTTGG C^C[AT][AT]GG
XcmI 1479 CCAGTTCCTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 505 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspI 331 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
849 GACCCAGTC
BsaWI 1465 TCCGGA [AT]^CCGG[AT]
1935 ACCGGT
BsgI 1931 CTGCAC GTGCAG(16/14)
1652 GTGCAG
BsmI 1758 GAATGC GAATGC(1/-1)
1370 GCATTC
BspMI 419 ACCTGC ACCTGC(4/8)
1065 ACCTGC
BsrBI 195 GAGCGG CCGCTC(-3/-3)
233 GAGCGG
BsrDI 1908 CATTGC GCAATG(2/0)
1205 GCAATG
BstXI 974 CCAGTCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1649 CCAGTGCAGTGG
BstYI 1454 AGATCC [GA]^GATC[CT]
1693 GGATCT
HaeII 70 AGCGCC [GA]GCGC^[CT]
456 GGCGCT
MflI 1454 AGATCC [GA]^GATC[CT]
1693 GGATCT
NaeI 596 GCCGGC GCC^GGC
821 GCCGGC
NgoMIV 596 GCCGGC G^CCGGC
821 GCCGGC
PflFI 331 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
849 GACCCAGTC
TaqI 506 TCGA T^CGA
521 TCGA
Tth111I 331 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
849 GACCCAGTC
TthHB8I 506 TCGA T^CGA
521 TCGA
XhoII 1454 AGATCC [GA]^GATC[CT]
1693 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII EaeI EagI EarI
EclXI Eco52I EcoNI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI