Restriction map for
pFN21AE3774
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeI MamI MfeI MluNI MroI
MscI MseI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 248 GGTACC G^GTACC
AccI 190 GTCTAC GT^[AC][GT]AC
AccII 611 CGCG CG^CG
AcsI 51 GAATTT [GA]^AATT[CT]
AfaI 249 GTAC GT^AC
AhdI 553 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 51 GAATTT [GA]^AATT[CT]
Asp718I 248 GGTACC G^GTACC
AspEI 553 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 254 GGACC G^G[AT]CC
BanI 248 GGTACC G^G[CT][GA]CC
BanII 594 GGGCTC G[GA]GC[CT]^C
BbsI 183 GAAGAC GAAGAC(2/6)
BglII 221 AGATCT A^GATCT
BmyI 594 GGGCTC G[AGT]GC[ACT]^C
BpmI 26 CTGGAG CTGGAG(16/14)
BpuAI 183 GAAGAC GAAGAC(2/6)
BsaWI 251 ACCGGA [AT]^CCGG[AT]
BseRI 597 CTCCTC GAGGAG(10/8)
BsmAI 614 GTCTC GTCTC(1/5)
BsmBI 613 CGTCTC CGTCTC(1/5)
Bsp1286I 594 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 62 ACATGT A^CATGT
BsrBI 244 GAGCGG CCGCTC(-3/-3)
BssSI 199 CACGAG CACGAG(-5/-1)
BstF5I 540 GGATG GGATG(2/0)
BstUI 611 CGCG CG^CG
BstYI 221 AGATCT [GA]^GATC[CT]
CfoI 531 GCGC GCG^C
Eam1105I 553 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 284 CCATGG C^C[AT][AT]GG
FauI 207 GCGGG CCCGC(4/6)
FokI 540 GGATG GGATG(9/13)
HaeII 530 AGCGCC [GA]GCGC^[CT]
HapII 252 CCGG C^CGG
HgaI 612 GCGTC GACGC(5/10)
HhaI 531 GCGC GCG^C
HinP1I 531 GCGC G^CGC
HpaII 252 CCGG C^CGG
KpnI 248 GGTACC GGTAC^C
MaeII 136 ACGT A^CGT
MflI 221 AGATCT [GA]^GATC[CT]
MluI 610 ACGCGT A^CGCGT
MslI 294 CATTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 252 CCGG C^CGG
MvnI 611 CGCG CG^CG
NcoI 284 CCATGG C^CATGG
NspI 62 ACATGT [GA]CATG^[CT]
PvuII 233 CAGCTG CAG^CTG
RsaI 249 GTAC GT^AC
StuI 521 AGGCCT AGG^CCT
StyI 284 CCATGG C^C[AT][AT]GG
TfiI 565 GATTC G^A[AT]TC
Tsp509I 52 AATT ^AATT
XhoII 221 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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BsiYI 339 CCAAGACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
365 CCTACTCCTGG
BslI 339 CCAAGACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
365 CCTACTCCTGG
BsmFI 227 GGGAC GGGAC(10/14)
374 GGGAC
BssKI 371 CCTGG ^CC[ACGT]GG
534 CCTGG
BstNI 371 CCTGG CC^[AT]GG
534 CCTGG
Cac8I 449 GCCAGC GC[ACGT]^[ACGT]GC
519 GCAGGC
DdeI 353 CTCAG C^T[ACGT]AG
440 CTGAG
DpnII 222 GATC ^GATC
357 GATC
EcoRII 371 CCTGG ^CC[AT]GG
534 CCTGG
MboI 222 GATC ^GATC
357 GATC
MspA1I 233 CAGCTG C[AC]G^C[GT]G
631 CCGCTG
MvaI 371 CCTGG CC^[AT]GG
534 CCTGG
NdeII 222 GATC ^GATC
357 GATC
PleI 29 GAGTC GAGTC(4/5)
322 GAGTC
PstI 217 CTGCAG CTGCA^G
637 CTGCAG
Sau3AI 222 GATC ^GATC
357 GATC
ScrFI 371 CCTGG CC^[ACGT]GG
534 CCTGG
SfcI 217 CTGCAG C^T[GA][CT]AG
637 CTGCAG
TaqI 170 TCGA T^CGA
586 TCGA
TthHB8I 170 TCGA T^CGA
586 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AflIII AluI BbvI BsaJI
BsrI Cfr13I DsaI EarI Fnu4HI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboII MnlI MwoI NlaIII
NlaIV Sau96I SfaNI TseI Tsp45I
TspRI