Restriction map for
pFN21AE1120
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 800 CGCG CG^CG
AflIII 617 ACGTGT A^C[GA][CT]GT
AspHI 706 GTGCTC G[AT]GC[AT]^C
AvaI 640 CCCGGG C^[CT]CG[GA]G
BamHI 269 GGATCC G^GATCC
BbsI 597 GAAGAC GAAGAC(2/6)
BmyI 706 GTGCTC G[AGT]GC[ACT]^C
BpuAI 597 GAAGAC GAAGAC(2/6)
BsgI 646 GTGCAG GTGCAG(16/14)
BsiHKAI 706 GTGCTC G[AT]GC[AT]^C
BsmBI 396 CGTCTC CGTCTC(1/5)
BsmFI 843 GGGAC GGGAC(10/14)
BsmI 826 GCATTC GAATGC(1/-1)
BsoBI 640 CCCGGG C^[CT]CG[GA]G
Bsp1286I 706 GTGCTC G[AGT]GC[ACT]^C
BsrBI 740 GAGCGG CCGCTC(-3/-3)
BstAPI 862 GCATGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 672 GGTCACC G^GT[ACGT]ACC
BstPI 672 GGTCACC G^GT[ACGT]ACC
BstUI 800 CGCG CG^CG
BstXI 339 CCAGGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 694 CTCAG C^T[ACGT]AG
DraII 111 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 588 CCACGG C^C[GA][CT]GG
EcoO109I 111 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 672 GGTCACC G^GT[ACGT]ACC
HaeII 1045 AGCGCC [GA]GCGC^[CT]
HgaI 798 GACGC GACGC(5/10)
HincII 1021 GTTGAC GT[CT]^[GA]AC
HindII 1021 GTTGAC GT[CT]^[GA]AC
MaeII 617 ACGT A^CGT
MvnI 800 CGCG CG^CG
NaeI 918 GCCGGC GCC^GGC
NgoMIV 918 GCCGGC G^CCGGC
NspI 862 GCATGC [GA]CATG^[CT]
PflMI 167 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 763 GAGTC GAGTC(4/5)
SexAI 338 ACCAGGT A^CC[AT]GGT
SmaI 640 CCCGGG CCC^GGG
SphI 862 GCATGC GCATG^C
TfiI 769 GAATC G^A[AT]TC
Tsp509I 130 AATT ^AATT
Van91I 167 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 734 CCATCCGAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 640 CCCGGG C^CCGGG
XmaI 640 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 819 GGTCC G^G[AT]CC
844 GGACC
BcnI 640 CCCGG CC^[GC]GG
641 CCGGG
BglI 496 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
913 GCCCTGCCGGC
BpmI 1041 CTCCAG CTGGAG(16/14)
382 CTGGAG
BspMI 676 ACCTGC ACCTGC(4/8)
575 GCAGGT
BsrFI 918 GCCGGC [GA]^CCGG[CT]
977 ACCGGC
BstBI 118 TTCGAA TT^CGAA
986 TTCGAA
BstYI 269 GGATCC [GA]^GATC[CT]
530 GGATCT
Cfr10I 918 GCCGGC [GA]^CCGG[CT]
977 ACCGGC
FauI 258 CCCGC CCCGC(4/6)
555 CCCGC
HinfI 763 GAGTC G^A[ACGT]TC
769 GAATC
MaeIII 673 GTCAC ^GT[ACGT]AC
796 GTGAC
MflI 269 GGATCC [GA]^GATC[CT]
530 GGATCT
MslI 699 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
804 CATGGTGGTG
MspA1I 259 CCGCTG C[AC]G^C[GT]G
624 CAGCGG
NciI 640 CCCGG CC^[GC]GG
641 CCGGG
NspV 118 TTCGAA TT^CGAA
986 TTCGAA
SfuI 118 TTCGAA TT^CGAA
986 TTCGAA
TaqI 119 TCGA T^CGA
987 TCGA
Tsp45I 673 GTCAC ^GT[GC]AC
796 GTGAC
TspRI 10 CAGTG CA[GC]TG[ACGT][ACGT]^
168 CAGTG
TthHB8I 119 TCGA T^CGA
987 TCGA
XhoII 269 GGATCC [GA]^GATC[CT]
530 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BalI BbvI BsaI BsaJI BsiYI
BslI BsmAI BsrDI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MboI MboII MluNI
MnlI MscI MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI