Restriction map for
pFN21AE9544
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI ApaLI
ApoI AscI Asp700I AspEI AspI
AviII AvrII BamHI BbsI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI Ksp632I MamI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PshAI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
SexAI SfaNI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 715 GTCTAC GT^[AC][GT]AC
AflIII 692 ACACGT A^C[GA][CT]GT
Aor51HI 110 AGCGCT AGC^GCT
AseI 925 ATTAAT AT^TAAT
AsnI 925 ATTAAT AT^TAAT
AspHI 736 GTGCTC G[AT]GC[AT]^C
BalI 318 TGGCCA TGG^CCA
BbeI 1099 GGCGCC GGCGC^C
BbrPI 693 CACGTG CAC^GTG
BglII 1130 AGATCT A^GATCT
BpmI 58 CTCCAG CTGGAG(16/14)
BsaAI 693 CACGTG [CT]AC^GT[GA]
BsiHKAI 736 GTGCTC G[AT]GC[AT]^C
Bsp1407I 419 TGTACA T^GTACA
BspHI 911 TCATGA T^CATGA
BsrBI 403 GAGCGG CCGCTC(-3/-3)
BsrDI 496 CATTGC GCAATG(2/0)
BsrFI 1004 ACCGGC [GA]^CCGG[CT]
BsrGI 419 TGTACA T^GTACA
BssHII 1275 GCGCGC G^CGCGC
BstF5I 906 CATCC GGATG(2/0)
Cfr10I 1004 ACCGGC [GA]^CCGG[CT]
Eco47III 110 AGCGCT AGC^GCT
EcoNI 1245 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 906 CATCC GGATG(9/13)
HphI 822 GGTGA GGTGA(8/7)
KasI 1099 GGCGCC G^GCGCC
MluNI 318 TGGCCA TGG^CCA
MscI 318 TGGCCA TGG^CCA
MseI 926 TTAA T^TAA
NarI 1099 GGCGCC GG^CGCC
NheI 459 GCTAGC G^CTAGC
PmaCI 693 CACGTG CAC^GTG
PmlI 693 CACGTG CAC^GTG
PshBI 925 ATTAAT AT^TAAT
PvuII 22 CAGCTG CAG^CTG
RcaI 911 TCATGA T^CATGA
ScaI 671 AGTACT AGT^ACT
SfoI 1099 GGCGCC GGC^GCC
SmlI 474 CTTGAG C^T[CT][GA]AG
SspBI 419 TGTACA T^GTACA
Tru9I 926 TTAA T^TAA
Tsp509I 928 AATT ^AATT
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# TWO CUT ENZYMES ( 34 enzymes) #
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AcyI 712 GGCGTC G[GA]^CG[CT]C
1099 GGCGCC
AlwI 15 GATCC GGATC(4/5)
1206 GATCC
AlwNI 22 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1355 CAGGGCCTG
ApaI 794 GGGCCC GGGCC^C
1286 GGGCCC
BanII 794 GGGCCC G[GA]GC[CT]^C
1286 GGGCCC
BfaI 460 CTAG C^TAG
503 CTAG
BglI 302 GCCAGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1279 GCCTTCCGGGC
BsaHI 712 GGCGTC G[GA]^CG[CT]C
1099 GGCGCC
BsmI 947 GCATTC GAATGC(1/-1)
956 GCATTC
BsrI 303 CCAGT ACTGG(1/-1)
669 CCAGT
BssSI 1057 CACGAG CACGAG(-5/-1)
739 CTCGTG
BstYI 1130 AGATCT [GA]^GATC[CT]
1205 AGATCC
EaeI 318 TGGCCA [CT]^GGCC[GA]
554 CGGCCA
EcoT14I 146 CCTTGG C^C[AT][AT]GG
600 CCTTGG
HaeII 110 AGCGCT [GA]GCGC^[CT]
1099 GGCGCC
Hin1I 712 GGCGTC G[GA]^CG[CT]C
1099 GGCGCC
KspI 155 CCGCGG CCGC^GG
1329 CCGCGG
MaeI 460 CTAG C^TAG
503 CTAG
MboII 366 GAAGA GAAGA(8/7)
1133 TCTTC
MflI 1130 AGATCT [GA]^GATC[CT]
1205 AGATCC
MslI 271 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
906 CATCCTCATG
PstI 19 CTGCAG CTGCA^G
284 CTGCAG
SacII 155 CCGCGG CCGC^GG
1329 CCGCGG
SfcI 19 CTGCAG C^T[GA][CT]AG
284 CTGCAG
SmaI 1094 CCCGGG CCC^GGG
1308 CCCGGG
StuI 171 AGGCCT AGG^CCT
374 AGGCCT
StyI 146 CCTTGG C^C[AT][AT]GG
600 CCTTGG
TaqI 194 TCGA T^CGA
585 TCGA
Tsp45I 664 GTGAC ^GT[GC]AC
1078 GTGAC
TthHB8I 194 TCGA T^CGA
585 TCGA
XcmI 1025 CCACGCTGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1373 CCAGCCGGGAGTTGG
XhoII 1130 AGATCT [GA]^GATC[CT]
1205 AGATCC
XmaCI 1094 CCCGGG C^CCGGG
1308 CCCGGG
XmaI 1094 CCCGGG C^CCGGG
1308 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
Asp718I AvaI AvaII BanI BbvCI
BbvI BcnI BmyI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BssKI
BstAPI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI KpnI MaeII MaeIII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PpuMI RsaI Sau3AI
Sau96I ScrFI TseI TspRI Van91I