Restriction map for
pFN21AE3704
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI ApaI
AscI Asp718I AspEI AvaI AviII
AvrII BalI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BspLU11I BsrBI
BssHII BstXI Bsu36I ClaI CpoI
DraI DraII DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoO109I
EcoRV FbaI FseI FspI HgaI
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NdeI NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 1547 GGCGCC G[GA]^CG[CT]C
Alw44I 3007 GTGCAC G^TGCAC
AlwNI 3314 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1613 AGCGCT AGC^GCT
ApaLI 3007 GTGCAC G^TGCAC
AspI 2854 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1547 GGCGCC G^G[CT][GA]CC
BbeI 1547 GGCGCC GGCGC^C
BglII 2391 AGATCT A^GATCT
BlpI 1295 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1295 GCTCAGC GC^T[ACGT]AGC
BsaHI 1547 GGCGCC G[GA]^CG[CT]C
BsmBI 13 GAGACG CGTCTC(1/5)
BsmI 906 GCATTC GAATGC(1/-1)
Bsp1407I 21 TGTACA T^GTACA
BspHI 2961 TCATGA T^CATGA
BsrFI 2034 GCCGGC [GA]^CCGG[CT]
BsrGI 21 TGTACA T^GTACA
Bst1107I 778 GTATAC GTA^TAC
BstAPI 3230 GCATTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3165 TTCGAA TT^CGAA
BstEII 2689 GGTGACC G^GT[ACGT]ACC
BstPI 2689 GGTGACC G^GT[ACGT]ACC
BstZ17I 778 GTATAC GTA^TAC
CelII 1295 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2034 GCCGGC [GA]^CCGG[CT]
DraIII 2414 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1613 AGCGCT AGC^GCT
EcoNI 1257 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2689 GGTGACC G^GT[ACGT]ACC
EcoRI 3423 GAATTC G^AATTC
Hin1I 1547 GGCGCC G[GA]^CG[CT]C
HincII 2176 GTTGAC GT[CT]^[GA]AC
HindII 2176 GTTGAC GT[CT]^[GA]AC
KasI 1547 GGCGCC G^GCGCC
NaeI 2034 GCCGGC GCC^GGC
NarI 1547 GGCGCC GG^CGCC
NcoI 1496 CCATGG C^CATGG
NgoMIV 2034 GCCGGC G^CCGGC
NspV 3165 TTCGAA TT^CGAA
PflFI 2854 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 2407 CTGCAG CTGCA^G
RcaI 2961 TCATGA T^CATGA
ScaI 1217 AGTACT AGT^ACT
SexAI 448 ACCTGGT A^CC[AT]GGT
SfoI 1547 GGCGCC GGC^GCC
SfuI 3165 TTCGAA TT^CGAA
SmlI 631 CTTGAG C^T[CT][GA]AG
SspBI 21 TGTACA T^GTACA
SspI 646 AATATT AAT^ATT
Tth111I 2854 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 196 CCAGTGGATGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 778 GTATAC GT^[AC][GT]AC
2284 GTCTAC
AccII 2320 CGCG CG^CG
2456 CGCG
AseI 649 ATTAAT AT^TAAT
3271 ATTAAT
AsnI 649 ATTAAT AT^TAAT
3271 ATTAAT
Asp700I 1066 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3376 GAAAGATTTC
AvaII 734 GGACC G^G[AT]CC
1173 GGTCC
BamHI 1083 GGATCC G^GATCC
1187 GGATCC
BanII 1138 GAGCTC G[GA]GC[CT]^C
1293 GAGCTC
BfaI 115 CTAG C^TAG
1867 CTAG
BsaI 896 GGTCTC GGTCTC(1/5)
2467 GGTCTC
BssSI 1290 CACGAG CACGAG(-5/-1)
2429 CTCGTG
BstUI 2320 CGCG CG^CG
2456 CGCG
EcoT22I 392 ATGCAT ATGCA^T
1851 ATGCAT
FauI 2454 CCCGC CCCGC(4/6)
918 GCGGG
HindIII 572 AAGCTT A^AGCTT
2611 AAGCTT
MaeI 115 CTAG C^TAG
1867 CTAG
MvnI 2320 CGCG CG^CG
2456 CGCG
NsiI 392 ATGCAT ATGCA^T
1851 ATGCAT
PflMI 196 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
998 CCATCCTTTGG
PshAI 544 GACGTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1535 GACCTGTGTC
PshBI 649 ATTAAT AT^TAAT
3271 ATTAAT
SacI 1138 GAGCTC GAGCT^C
1293 GAGCTC
SapI 1135 GAAGAGC GCTCTTC(1/4)
3088 GAAGAGC
SphI 1849 GCATGC GCATG^C
2199 GCATGC
Van91I 196 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
998 CCATCCTTTGG
XmnI 1066 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3376 GAAAGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BbsI BbvI BciVI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII