Restriction map for
pFN21AE4264
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BcgI BclI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BsrFI BssHII BssSI Bst1107I
BstAPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 2531 GTCTAC GT^[AC][GT]AC
AccII 1291 CGCG CG^CG
AcyI 2528 GGCGTC G[GA]^CG[CT]C
AvaI 1820 CCCGGG C^[CT]CG[GA]G
AvaII 2616 GGACC G^G[AT]CC
BamHI 207 GGATCC G^GATCC
BbvCI 2629 GCTGAGG CCTCAGC(-5/-2)
BsaHI 2528 GGCGTC G[GA]^CG[CT]C
BsaI 1662 GAGACC GGTCTC(1/5)
BsaWI 743 TCCGGT [AT]^CCGG[AT]
BsmBI 3001 GAGACG CGTCTC(1/5)
BsmFI 1699 GTCCC GGGAC(10/14)
BsoBI 1820 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2126 TGTACA T^GTACA
BspLU11I 2162 ACATGT A^CATGT
BsrDI 2074 CATTGC GCAATG(2/0)
BsrGI 2126 TGTACA T^GTACA
BstEII 2589 GGTGACC G^GT[ACGT]ACC
BstPI 2589 GGTGACC G^GT[ACGT]ACC
BstUI 1291 CGCG CG^CG
BstXI 2861 CCAATAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2097 GCGC GCG^C
DraI 955 TTTAAA TTT^AAA
DraII 2615 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 416 TGGCCG [CT]^GGCC[GA]
EcoO109I 2615 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2589 GGTGACC G^GT[ACGT]ACC
FauI 2517 GCGGG CCCGC(4/6)
HaeII 2096 AGCGCC [GA]GCGC^[CT]
HhaI 2097 GCGC GCG^C
Hin1I 2528 GGCGTC G[GA]^CG[CT]C
HinP1I 2097 GCGC G^CGC
HincII 2316 GTTGAC GT[CT]^[GA]AC
HindII 2316 GTTGAC GT[CT]^[GA]AC
HindIII 392 AAGCTT A^AGCTT
MvnI 1291 CGCG CG^CG
NdeI 1981 CATATG CA^TATG
PpuMI 2615 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1281 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1339 AGTACT AGT^ACT
SexAI 1760 ACCTGGT A^CC[AT]GGT
SmaI 1820 CCCGGG CCC^GGG
SspBI 2126 TGTACA T^GTACA
XmaCI 1820 CCCGGG C^CCGGG
XmaI 1820 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflII 501 CTTAAG C^TTAAG
519 CTTAAG
AflIII 735 ACACGT A^C[GA][CT]GT
2162 ACATGT
BanI 2332 GGTGCC G^G[CT][GA]CC
2912 GGTGCC
BciVI 773 GGATAC GTATCC(6/5)
1585 GTATCC
BfrI 501 CTTAAG C^TTAAG
519 CTTAAG
BsaBI 510 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
984 GATTGTCATC
BsmI 1778 GCATTC GAATGC(1/-1)
1999 GCATTC
BspMI 2237 ACCTGC ACCTGC(4/8)
2309 ACCTGC
BstBI 2062 TTCGAA TT^CGAA
2298 TTCGAA
HgaI 3003 GACGC GACGC(5/10)
2529 GCGTC
MamI 510 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
984 GATTGTCATC
NheI 189 GCTAGC G^CTAGC
1734 GCTAGC
NspI 1082 ACATGC [GA]CATG^[CT]
2162 ACATGT
NspV 2062 TTCGAA TT^CGAA
2298 TTCGAA
SacI 721 GAGCTC GAGCT^C
3162 GAGCTC
SapI 723 GCTCTTC GCTCTTC(1/4)
1708 GCTCTTC
SfcI 1229 CTGTAG C^T[GA][CT]AG
1463 CTACAG
SfuI 2062 TTCGAA TT^CGAA
2298 TTCGAA
XcmI 482 CCAGGAGTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3050 CCAAACTCCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI