Restriction map for
pFN21AE3333
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AcyI AflII AflIII
AlwNI AocI ApaI AscI Asp718I
AspI AviII AvrII BbeI BbvCI
BcgI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII EagI EclXI Eco52I Eco81I
EcoO65I EcoRI FauI FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PmeI PshAI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 1631 TCCGGA T^CCGGA
AclI 2765 AACGTT AA^CGTT
AgeI 2560 ACCGGT A^CCGGT
Aor51HI 1557 AGCGCT AGC^GCT
AseI 2055 ATTAAT AT^TAAT
AsnI 2055 ATTAAT AT^TAAT
BciVI 896 GTATCC GTATCC(6/5)
BclI 2369 TGATCA T^GATCA
BseAI 1631 TCCGGA T^CCGGA
BspEI 1631 TCCGGA T^CCGGA
BstXI 1113 CCAAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1490 GCGTGC GC[ACGT]^[ACGT]GC
DraI 2407 TTTAAA TTT^AAA
DrdI 1952 GACCTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1557 AGCGCT AGC^GCT
EcoNI 633 CCTAGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1528 GATATC GAT^ATC
EcoT22I 2121 ATGCAT ATGCA^T
FbaI 2369 TGATCA T^GATCA
HgaI 28 GACGC GACGC(5/10)
MroI 1631 TCCGGA T^CCGGA
MslI 2557 CACACCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 544 CAGCTG C[AC]G^C[GT]G
NsiI 2121 ATGCAT ATGCA^T
NspI 1232 ACATGC [GA]CATG^[CT]
PinAI 2560 ACCGGT A^CCGGT
PshBI 2055 ATTAAT AT^TAAT
Psp1406I 2765 AACGTT AA^CGTT
PvuII 544 CAGCTG CAG^CTG
SacI 2526 GAGCTC GAGCT^C
SapI 1578 GAAGAGC GCTCTTC(1/4)
SgrAI 2559 CACCGGTG C[GA]^CCGG[CT]G
SmlI 253 CTTGAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccI 816 GTCTAC GT^[AC][GT]AC
1961 GTCTAC
AccII 30 CGCG CG^CG
1719 CGCG
AfaI 2090 GTAC GT^AC
2696 GTAC
AhdI 392 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
864 GACTCTAGGTC
Alw44I 412 GTGCAC G^TGCAC
1492 GTGCAC
ApaLI 412 GTGCAC G^TGCAC
1492 GTGCAC
AspEI 392 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
864 GACTCTAGGTC
BamHI 1842 GGATCC G^GATCC
2010 GGATCC
BanI 1459 GGTGCC G^G[CT][GA]CC
2455 GGTGCC
BbrPI 697 CACGTG CAC^GTG
1702 CACGTG
BsaAI 697 CACGTG [CT]AC^GT[GA]
1702 CACGTG
BsgI 809 GTGCAG GTGCAG(16/14)
959 GTGCAG
BsrFI 212 GCCGGT [GA]^CCGG[CT]
2560 ACCGGT
BstAPI 952 GCAAGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1285 GCAGCCCGTGC
BstUI 30 CGCG CG^CG
1719 CGCG
BstYI 1842 GGATCC [GA]^GATC[CT]
2010 GGATCC
Cfr10I 212 GCCGGT [GA]^CCGG[CT]
2560 ACCGGT
DsaI 859 CCATGG C^C[GA][CT]GG
1455 CCATGG
Eam1105I 392 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
864 GACTCTAGGTC
HaeII 1557 AGCGCT [GA]GCGC^[CT]
2623 AGCGCC
MflI 1842 GGATCC [GA]^GATC[CT]
2010 GGATCC
MvnI 30 CGCG CG^CG
1719 CGCG
NcoI 859 CCATGG C^CATGG
1455 CCATGG
PmaCI 697 CACGTG CAC^GTG
1702 CACGTG
PmlI 697 CACGTG CAC^GTG
1702 CACGTG
RsaI 2090 GTAC GT^AC
2696 GTAC
StuI 1094 AGGCCT AGG^CCT
2218 AGGCCT
Tsp45I 166 GTGAC ^GT[GC]AC
646 GTGAC
XhoII 1842 GGATCC [GA]^GATC[CT]
2010 GGATCC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I AspHI AvaI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XmnI