Restriction map for
pFN21AB0448
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AvaI AviII AvrII BbeI
BbrPI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I CpoI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FseI FspI HpaI KasI KpnI
KspI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI StuI SwaI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 998 GACGCC G[GA]^CG[CT]C
AhdI 2534 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2039 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2534 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2318 GTGCTC G[AT]GC[AT]^C
AspI 2644 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1256 GGATCC G^GATCC
BanI 1410 GGTGCC G^G[CT][GA]CC
BclI 32 TGATCA T^GATCA
BsaAI 2527 TACGTG [CT]AC^GT[GA]
BsaBI 766 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 998 GACGCC G[GA]^CG[CT]C
BsaI 2642 GAGACC GGTCTC(1/5)
BsiHKAI 2318 GTGCTC G[AT]GC[AT]^C
BsmI 1531 GCATTC GAATGC(1/-1)
BspDI 110 ATCGAT AT^CGAT
BspMI 1523 ACCTGC ACCTGC(4/8)
BsrDI 2287 GCAATG GCAATG(2/0)
BstYI 1256 GGATCC [GA]^GATC[CT]
ClaI 110 ATCGAT AT^CGAT
DraI 982 TTTAAA TTT^AAA
Eam1105I 2534 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1785 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 347 ATGCAT ATGCA^T
FauI 1860 CCCGC CCCGC(4/6)
FbaI 32 TGATCA T^GATCA
Hin1I 998 GACGCC G[GA]^CG[CT]C
HindIII 211 AAGCTT A^AGCTT
MamI 766 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 152 CAATTG C^AATTG
MflI 1256 GGATCC [GA]^GATC[CT]
MunI 152 CAATTG C^AATTG
NdeI 1178 CATATG CA^TATG
NsiI 347 ATGCAT ATGCA^T
NspI 1053 GCATGT [GA]CATG^[CT]
PflFI 2644 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2334 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1691 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmlI 1116 CTCAAG C^T[CT][GA]AG
SspI 1472 AATATT AAT^ATT
Tth111I 2644 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1256 GGATCC [GA]^GATC[CT]
XmnI 2039 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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BbvCI 1769 CCTCAGC CCTCAGC(-5/-2)
2440 CCTCAGC
BsgI 1763 CTGCAC GTGCAG(16/14)
2125 GTGCAG
BstXI 1916 CCAAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2393 CCACAGCTGTGG
CfoI 1782 GCGC GCG^C
2726 GCGC
DraII 2259 GGGCCCT [GA]G^G[ACGT]CC[CT]
2334 AGGACCT
DraIII 2492 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2603 CACATCGTG
DsaI 238 CCATGG C^C[GA][CT]GG
1221 CCATGG
EcoO109I 2259 GGGCCCT [GA]G^G[ACGT]CC[CT]
2334 AGGACCT
HaeII 1781 AGCGCC [GA]GCGC^[CT]
2725 GGCGCT
HhaI 1782 GCGC GCG^C
2726 GCGC
HinP1I 1782 GCGC G^CGC
2726 GCGC
HphI 769 GGTGA GGTGA(8/7)
2566 TCACC
NcoI 238 CCATGG C^CATGG
1221 CCATGG
SapI 694 GAAGAGC GCTCTTC(1/4)
1063 GAAGAGC
SbfI 1524 CCTGCAGG CCTGCA^GG
2134 CCTGCAGG
Sse8387I 1524 CCTGCAGG CCTGCA^GG
2134 CCTGCAGG
TaqI 111 TCGA T^CGA
163 TCGA
TthHB8I 111 TCGA T^CGA
163 TCGA
XcmI 1081 CCAGCCTTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1397 CCAGTAAGAGAGTGG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Bsu36I Cac8I Cfr13I
DdeI DpnII EaeI EarI Eco81I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaII ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI