Restriction map for
pFN21AE4230
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I FbaI FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI KspI MaeII MamI MluI
MluNI MroI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1459 GTAGAC GT^[AC][GT]AC
AccII 139 CGCG CG^CG
Alw44I 1604 GTGCAC G^TGCAC
AlwI 176 GATCC GGATC(4/5)
ApaLI 1604 GTGCAC G^TGCAC
Asp700I 27 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 1312 GGATAC GTATCC(6/5)
BsmBI 99 GAGACG CGTCTC(1/5)
BsmI 1431 GCATTC GAATGC(1/-1)
BspHI 2291 TCATGA T^CATGA
BspMI 191 GCAGGT ACCTGC(4/8)
BsrBI 1093 GAGCGG CCGCTC(-3/-3)
BsrFI 516 GCCGGT [GA]^CCGG[CT]
BstAPI 2145 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 139 CGCG CG^CG
CfoI 915 GCGC GCG^C
Cfr10I 516 GCCGGT [GA]^CCGG[CT]
DraI 1329 TTTAAA TTT^AAA
EcoRV 258 GATATC GAT^ATC
EcoT22I 1429 ATGCAT ATGCA^T
FauI 54 GCGGG CCCGC(4/6)
HgaI 10 GACGC GACGC(5/10)
HhaI 915 GCGC GCG^C
HinP1I 915 GCGC G^CGC
HincII 2158 GTTGAC GT[CT]^[GA]AC
HindII 2158 GTTGAC GT[CT]^[GA]AC
MfeI 293 CAATTG C^AATTG
MunI 293 CAATTG C^AATTG
MvnI 139 CGCG CG^CG
NsiI 1429 ATGCAT ATGCA^T
PshAI 1969 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2047 CTGCAG CTGCA^G
RcaI 2291 TCATGA T^CATGA
SmaI 1129 CCCGGG CCC^GGG
XmaCI 1129 CCCGGG C^CCGGG
XmaI 1129 CCCGGG C^CCGGG
XmnI 27 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 329 GTAC GT^AC
2244 GTAC
AspHI 671 GTGCTC G[AT]GC[AT]^C
1604 GTGCAC
AvaII 360 GGACC G^G[AT]CC
609 GGTCC
BbsI 820 GAAGAC GAAGAC(2/6)
2124 GAAGAC
BfaI 188 CTAG C^TAG
215 CTAG
BglII 1680 AGATCT A^GATCT
2109 AGATCT
BpuAI 820 GAAGAC GAAGAC(2/6)
2124 GAAGAC
BsiHKAI 671 GTGCTC G[AT]GC[AT]^C
1604 GTGCAC
BsrDI 1070 GCAATG GCAATG(2/0)
1075 GCAATG
EcoNI 702 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1941 CCTGGAGGAGG
EcoRI 1398 GAATTC G^AATTC
2232 GAATTC
EcoT14I 241 CCAAGG C^C[AT][AT]GG
717 CCAAGG
MaeI 188 CTAG C^TAG
215 CTAG
NspI 59 ACATGC [GA]CATG^[CT]
1767 GCATGT
RsaI 329 GTAC GT^AC
2244 GTAC
SexAI 2246 ACCTGGT A^CC[AT]GGT
2309 ACCAGGT
SmlI 734 CTCAAG C^T[CT][GA]AG
1918 CTCAAG
StyI 241 CCAAGG C^C[AT][AT]GG
717 CCAAGG
TaqI 967 TCGA T^CGA
1477 TCGA
TthHB8I 967 TCGA T^CGA
1477 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AluI AlwNI AocI
ApoI AvaI BanI BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII EarI Eco81I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII