Restriction map for
pFN21AE9628
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII AccIII AcyI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiWI BsmI
Bsp1407I BspEI BspHI BspMI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I CpoI DraI DrdI Eam1105I
Eco47III EcoO65I EcoT22I FseI FspI
HgaI Hin1I HindIII HpaI KasI
KspI MluI MluNI MroI MscI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PvuI RcaI RsrII SacI
SacII SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 772 GGTACC G^GTACC
AccII 22 CGCG CG^CG
AclI 436 AACGTT AA^CGTT
AflIII 2513 ACATGT A^C[GA][CT]GT
AocI 241 CCTGAGG CC^T[ACGT]AGG
AseI 951 ATTAAT AT^TAAT
AsnI 951 ATTAAT AT^TAAT
Asp718I 772 GGTACC G^GTACC
BbsI 1030 GAAGAC GAAGAC(2/6)
BbvCI 1195 CCTCAGC CCTCAGC(-5/-2)
BciVI 1742 GTATCC GTATCC(6/5)
BclI 489 TGATCA T^GATCA
BglII 2093 AGATCT A^GATCT
BpuAI 1030 GAAGAC GAAGAC(2/6)
BsiEI 136 CGGCCG CG[GA][CT]^CG
BsmBI 2632 CGTCTC CGTCTC(1/5)
BspDI 2139 ATCGAT AT^CGAT
BspLU11I 2513 ACATGT A^CATGT
BsrBI 565 GAGCGG CCGCTC(-3/-3)
BssSI 2584 CTCGTG CACGAG(-5/-1)
BstUI 22 CGCG CG^CG
BstYI 2093 AGATCT [GA]^GATC[CT]
Bsu36I 241 CCTGAGG CC^T[ACGT]AGG
ClaI 2139 ATCGAT AT^CGAT
DraIII 1169 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1604 CCATGG C^C[GA][CT]GG
EagI 136 CGGCCG C^GGCCG
EclXI 136 CGGCCG C^GGCCG
Eco52I 136 CGGCCG C^GGCCG
Eco81I 241 CCTGAGG CC^T[ACGT]AGG
EcoNI 1911 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1312 GAATTC G^AATTC
EcoT14I 1604 CCATGG C^C[AT][AT]GG
FauI 38 CCCGC CCCGC(4/6)
FbaI 489 TGATCA T^GATCA
KpnI 772 GGTACC GGTAC^C
MfeI 962 CAATTG C^AATTG
MflI 2093 AGATCT [GA]^GATC[CT]
MunI 962 CAATTG C^AATTG
MvnI 22 CGCG CG^CG
NcoI 1604 CCATGG C^CATGG
PpuMI 75 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1860 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 951 ATTAAT AT^TAAT
Psp1406I 436 AACGTT AA^CGTT
PvuII 1198 CAGCTG CAG^CTG
SapI 2071 GAAGAGC GCTCTTC(1/4)
SexAI 302 ACCTGGT A^CC[AT]GGT
SmaI 1401 CCCGGG CCC^GGG
StyI 1604 CCATGG C^C[AT][AT]GG
XhoII 2093 AGATCT [GA]^GATC[CT]
XmaCI 1401 CCCGGG C^CCGGG
XmaI 1401 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 126 GTCGAC GT^[AC][GT]AC
2226 GTCGAC
AlwI 91 GGATC GGATC(4/5)
958 GGATC
AvaI 26 CCCGAG C^[CT]CG[GA]G
1401 CCCGGG
BanI 66 GGCACC G^G[CT][GA]CC
772 GGTACC
BpmI 2342 CTCCAG CTGGAG(16/14)
2089 CTGGAG
BsaBI 463 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1737 GATTTGTATC
BsoBI 26 CCCGAG C^[CT]CG[GA]G
1401 CCCGGG
BsrDI 2198 CATTGC GCAATG(2/0)
1510 GCAATG
Cac8I 37 GCCCGC GC[ACGT]^[ACGT]GC
2574 GCTGGC
DraII 75 GGGACCC [GA]G^G[ACGT]CC[CT]
1154 AGGGCCT
EaeI 136 CGGCCG [CT]^GGCC[GA]
553 CGGCCA
EarI 1406 GAAGAG CTCTTC(1/4)
2071 GAAGAG
EcoO109I 75 GGGACCC [GA]G^G[ACGT]CC[CT]
1154 AGGGCCT
EcoRV 402 GATATC GAT^ATC
1972 GATATC
HapII 135 CCGG C^CGG
1402 CCGG
HpaII 135 CCGG C^CGG
1402 CCGG
Ksp632I 1406 GAAGAG CTCTTC(1/4)
2071 GAAGAG
MamI 463 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1737 GATTTGTATC
MslI 1611 CATAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2274 CATTGTGGTG
MspA1I 139 CCGCTG C[AC]G^C[GT]G
1198 CAGCTG
MspI 135 CCGG C^CGG
1402 CCGG
NspI 333 GCATGT [GA]CATG^[CT]
2513 ACATGT
PflMI 1818 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2568 CCACAAGCTGG
SalI 126 GTCGAC G^TCGAC
2226 GTCGAC
SmlI 218 CTCAAG C^T[CT][GA]AG
2469 CTTGAG
Van91I 1818 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2568 CCACAAGCTGG
XcmI 519 CCATATATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1516 CCACTACATAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HincII HindII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I