Restriction map for
pFN21AE4052
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# NO CUT ENZYMES ( 168 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MamI MfeI MflI
MluI MroI MseI MspA1I MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tsp45I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 253 GAATTT [GA]^AATT[CT]
AflIII 80 ACGTGT A^C[GA][CT]GT
AocI 324 CCTTAGG CC^T[ACGT]AGG
ApoI 253 GAATTT [GA]^AATT[CT]
BalI 2 TGGCCA TGG^CCA
BbrPI 79 CACGTG CAC^GTG
BcnI 584 CCCGG CC^[GC]GG
BsaAI 79 CACGTG [CT]AC^GT[GA]
BseRI 650 CTCCTC GAGGAG(10/8)
BspHI 197 TCATGA T^CATGA
BsrFI 523 GCCGGC [GA]^CCGG[CT]
BstF5I 427 GGATG GGATG(2/0)
Bsu36I 324 CCTTAGG CC^T[ACGT]AGG
Cfr10I 523 GCCGGC [GA]^CCGG[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 273 GAAGAG CTCTTC(1/4)
Eco81I 324 CCTTAGG CC^T[ACGT]AGG
EcoRV 181 GATATC GAT^ATC
FauI 74 GCGGG CCCGC(4/6)
FokI 427 GGATG GGATG(9/13)
Ksp632I 273 GAAGAG CTCTTC(1/4)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 422 CACACGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 523 GCCGGC GCC^GGC
NciI 584 CCCGG CC^[GC]GG
NgoMIV 523 GCCGGC G^CCGGC
NlaIV 573 GGCCCC GG[ACGT]^[ACGT]CC
PleI 469 GAGTC GAGTC(4/5)
PmaCI 79 CACGTG CAC^GTG
PmlI 79 CACGTG CAC^GTG
PstI 340 CTGCAG CTGCA^G
RcaI 197 TCATGA T^CATGA
ScaI 224 AGTACT AGT^ACT
SfaNI 428 GATGC GCATC(5/9)
SmlI 557 CTCAAG C^T[CT][GA]AG
TaqI 472 TCGA T^CGA
Tsp509I 254 AATT ^AATT
TthHB8I 472 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
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AatII 48 GACGTC GACGT^C
629 GACGTC
AciI 37 CCGC CCGC(-3/-1)
74 GCGG
AluI 138 AGCT AG^CT
384 AGCT
BfaI 308 CTAG C^TAG
656 CTAG
BmyI 76 GGGCAC G[AGT]GC[ACT]^C
581 GTGCCC
BpmI 228 CTCCAG CTGGAG(16/14)
466 CTGGAG
BsaJI 99 CCTCGG C^C[ACGT][ACGT]GG
230 CCAGGG
BsmI 121 GAATGC GAATGC(1/-1)
190 GCATTC
Bsp1286I 76 GGGCAC G[AGT]GC[ACT]^C
581 GTGCCC
BstNI 186 CCAGG CC^[AT]GG
230 CCAGG
Cfr13I 526 GGCCC G^G[ACGT]CC
573 GGCCC
DdeI 325 CTTAG C^T[ACGT]AG
512 CTGAG
DpnII 368 GATC ^GATC
484 GATC
EcoRII 186 CCAGG ^CC[AT]GG
230 CCAGG
HapII 524 CCGG C^CGG
585 CCGG
HgaI 91 GACGC GACGC(5/10)
175 GACGC
HpaII 524 CCGG C^CGG
585 CCGG
MaeI 308 CTAG C^TAG
656 CTAG
MboI 368 GATC ^GATC
484 GATC
MspI 524 CCGG C^CGG
585 CCGG
MvaI 186 CCAGG CC^[AT]GG
230 CCAGG
NdeII 368 GATC ^GATC
484 GATC
PshAI 464 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
629 GACGTCTGTC
Sau3AI 368 GATC ^GATC
484 GATC
Sau96I 526 GGCCC G^G[ACGT]CC
573 GGCCC
SfcI 291 CTATAG C^T[GA][CT]AG
340 CTGCAG
TfiI 319 GAATC G^A[AT]TC
536 GAATC
TspRI 335 CAGTG CA[GC]TG[ACGT][ACGT]^
641 CACTG
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AcyI AfaI BbvI BsaHI BsiYI
BslI BsrI BssKI Cac8I Fnu4HI
HaeIII Hin1I HinfI HphI ItaI
MaeII MboII MnlI MwoI NlaIII
RsaI ScrFI TseI