Restriction map for
pFN21AE0980
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BciVI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiWI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI MvnI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1602 GGTACC G^GTACC
Asp700I 483 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1602 GGTACC G^GTACC
AvaII 912 GGACC G^G[AT]CC
BalI 1014 TGGCCA TGG^CCA
BanII 1334 GAGCTC G[GA]GC[CT]^C
BbvCI 659 CCTCAGC CCTCAGC(-5/-2)
BcgI 1599 CGAGGTACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1163 CCCGG CC^[GC]GG
BfaI 526 CTAG C^TAG
BglI 67 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1656 GCTCAGC GC^T[ACGT]AGC
BpmI 784 CTCCAG CTGGAG(16/14)
Bpu1102I 1656 GCTCAGC GC^T[ACGT]AGC
BsiEI 1045 CGACCG CG[GA][CT]^CG
BsmBI 1619 CGTCTC CGTCTC(1/5)
BsmI 1431 GCATTC GAATGC(1/-1)
BspHI 837 TCATGA T^CATGA
BspMI 1605 ACCTGC ACCTGC(4/8)
BsrFI 25 GCCGGC [GA]^CCGG[CT]
BstEII 633 GGTTACC G^GT[ACGT]ACC
BstNI 777 CCTGG CC^[AT]GG
BstPI 633 GGTTACC G^GT[ACGT]ACC
CelII 1656 GCTCAGC GC^T[ACGT]AGC
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
EcoO65I 633 GGTTACC G^GT[ACGT]ACC
EcoRII 777 CCTGG ^CC[AT]GG
EcoRV 1530 GATATC GAT^ATC
KpnI 1602 GGTACC GGTAC^C
MaeI 526 CTAG C^TAG
MluNI 1014 TGGCCA TGG^CCA
MscI 1014 TGGCCA TGG^CCA
MslI 1092 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 777 CCTGG CC^[AT]GG
NaeI 25 GCCGGC GCC^GGC
NciI 1163 CCCGG CC^[GC]GG
NdeI 800 CATATG CA^TATG
NgoMIV 25 GCCGGC G^CCGGC
NspI 1148 GCATGC [GA]CATG^[CT]
PshAI 895 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 88 CTGCAG CTGCA^G
PvuII 144 CAGCTG CAG^CTG
RcaI 837 TCATGA T^CATGA
SacI 1334 GAGCTC GAGCT^C
SapI 1426 GAAGAGC GCTCTTC(1/4)
ScaI 749 AGTACT AGT^ACT
SfcI 88 CTGCAG C^T[GA][CT]AG
SmlI 916 CTTGAG C^T[CT][GA]AG
SphI 1148 GCATGC GCATG^C
StuI 207 AGGCCT AGG^CCT
Tsp45I 170 GTGAC ^GT[GC]AC
XcmI 1091 CCATGGCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 483 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 215 GTCTAC GT^[AC][GT]AC
1116 GTCTAC
BbsI 1322 GAAGAC GAAGAC(2/6)
284 GTCTTC
BclI 305 TGATCA T^GATCA
1710 TGATCA
BpuAI 1322 GAAGAC GAAGAC(2/6)
284 GTCTTC
BseRI 1550 CTCCTC GAGGAG(10/8)
1357 GAGGAG
BsmAI 1284 GAGAC GTCTC(1/5)
1620 GTCTC
BssKI 777 CCTGG ^CC[ACGT]GG
1163 CCCGG
BstXI 1005 CCACTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1091 CCATGGCTGTGG
DraII 773 AGGGCCT [GA]G^G[ACGT]CC[CT]
1648 GGGGCCT
DsaI 1091 CCATGG C^C[GA][CT]GG
1347 CCATGG
EaeI 1014 TGGCCA [CT]^GGCC[GA]
1103 TGGCCG
EcoO109I 773 AGGGCCT [GA]G^G[ACGT]CC[CT]
1648 GGGGCCT
EcoRI 259 GAATTC G^AATTC
921 GAATTC
FbaI 305 TGATCA T^GATCA
1710 TGATCA
HapII 26 CCGG C^CGG
1164 CCGG
HpaII 26 CCGG C^CGG
1164 CCGG
HphI 1311 TCACC GGTGA(8/7)
1483 TCACC
MaeII 302 ACGT A^CGT
1492 ACGT
MspA1I 55 CCGCTG C[AC]G^C[GT]G
144 CAGCTG
MspI 26 CCGG C^CGG
1164 CCGG
MwoI 67 GCCATCGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1096 GCTGTGGTGGC
NcoI 1091 CCATGG C^CATGG
1347 CCATGG
ScrFI 777 CCTGG CC^[ACGT]GG
1163 CCCGG
SspI 485 AATATT AAT^ATT
813 AATATT
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI BanI BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BstF5I BstYI Cac8I
Cfr13I DdeI DpnII EarI EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII