Restriction map for
pFN21AB7881
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
EcoT22I FbaI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 564 GTCTAC GT^[AC][GT]AC
AccII 427 CGCG CG^CG
AflIII 761 ACATGT A^C[GA][CT]GT
AhdI 410 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 310 CCTGAGG CC^T[ACGT]AGG
ApaI 218 GGGCCC GGGCC^C
AspEI 410 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 135 TGGCCA TGG^CCA
BbsI 667 GAAGAC GAAGAC(2/6)
BbvCI 237 GCTGAGG CCTCAGC(-5/-2)
BpuAI 667 GAAGAC GAAGAC(2/6)
BsmBI 957 GAGACG CGTCTC(1/5)
BspLU11I 761 ACATGT A^CATGT
BsrDI 1475 GCAATG GCAATG(2/0)
BstEII 366 GGTGACC G^GT[ACGT]ACC
BstPI 366 GGTGACC G^GT[ACGT]ACC
BstUI 427 CGCG CG^CG
BstXI 837 CCAGCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 310 CCTGAGG CC^T[ACGT]AGG
CfoI 156 GCGC GCG^C
EaeI 135 TGGCCA [CT]^GGCC[GA]
Eam1105I 410 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 55 GAAGAG CTCTTC(1/4)
Eco81I 310 CCTGAGG CC^T[ACGT]AGG
EcoO65I 366 GGTGACC G^GT[ACGT]ACC
EcoRI 1613 GAATTC G^AATTC
EcoRV 1108 GATATC GAT^ATC
EcoT14I 257 CCAAGG C^C[AT][AT]GG
HhaI 156 GCGC GCG^C
HinP1I 156 GCGC G^CGC
Ksp632I 55 GAAGAG CTCTTC(1/4)
MluNI 135 TGGCCA TGG^CCA
MscI 135 TGGCCA TGG^CCA
MseI 332 TTAA T^TAA
MvnI 427 CGCG CG^CG
NspI 761 ACATGT [GA]CATG^[CT]
PpuMI 189 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 854 CAGCTG CAG^CTG
SfaNI 472 GCATC GCATC(5/9)
SmlI 889 CTTGAG C^T[CT][GA]AG
StyI 257 CCAAGG C^C[AT][AT]GG
Tru9I 332 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcsI 750 AAATTT [GA]^AATT[CT]
1613 GAATTC
ApoI 750 AAATTT [GA]^AATT[CT]
1613 GAATTC
AvaI 194 CCCGAG C^[CT]CG[GA]G
905 CCCGAG
AvaII 99 GGACC G^G[AT]CC
190 GGACC
BsmFI 98 GGGAC GGGAC(10/14)
681 GTCCC
BsoBI 194 CCCGAG C^[CT]CG[GA]G
905 CCCGAG
EcoNI 306 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
372 CCTATTAGAGG
FauI 936 GCGGG CCCGC(4/6)
1255 GCGGG
MspA1I 854 CAGCTG C[AC]G^C[GT]G
934 CAGCGG
PflMI 321 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
648 CCAGACTTTGG
TaqI 690 TCGA T^CGA
1686 TCGA
Tsp45I 367 GTGAC ^GT[GC]AC
546 GTCAC
TthHB8I 690 TCGA T^CGA
1686 TCGA
Van91I 321 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
648 CCAGACTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI BanII BbvI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI StuI TfiI
TseI Tsp509I TspRI XhoII XmnI