Restriction map for
pFN21AE4042
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MroI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 121 GTCTAC GT^[AC][GT]AC
AflIII 91 ACGTGT A^C[GA][CT]GT
Aor51HI 230 AGCGCT AGC^GCT
AspHI 517 GTGCTC G[AT]GC[AT]^C
BcnI 299 CCCGG CC^[GC]GG
BlpI 14 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 14 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 517 GTGCTC G[AT]GC[AT]^C
BsmFI 212 GGGAC GGGAC(10/14)
BspHI 336 TCATGA T^CATGA
BspMI 204 GCAGGT ACCTGC(4/8)
BssHII 541 GCGCGC G^CGCGC
CelII 14 GCTCAGC GC^T[ACGT]AGC
DpnII 537 GATC ^GATC
Eco47III 230 AGCGCT AGC^GCT
EcoNI 420 CCTGTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 230 AGCGCT [GA]GCGC^[CT]
HapII 300 CCGG C^CGG
HpaII 300 CCGG C^CGG
MaeIII 395 GTGAC ^GT[ACGT]AC
MboI 537 GATC ^GATC
MslI 556 CACCTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 218 CAGCTG C[AC]G^C[GT]G
MspI 300 CCGG C^CGG
NciI 299 CCCGG CC^[GC]GG
NdeII 537 GATC ^GATC
NlaIV 621 GGGTCC GG[ACGT]^[ACGT]CC
PvuII 218 CAGCTG CAG^CTG
RcaI 336 TCATGA T^CATGA
Sau3AI 537 GATC ^GATC
Tsp45I 395 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 540 CGCG CG^CG
542 CGCG
AvaII 305 GGTCC G^G[AT]CC
622 GGTCC
BalI 222 TGGCCA TGG^CCA
524 TGGCCA
BbsI 87 GAAGAC GAAGAC(2/6)
511 GTCTTC
BpuAI 87 GAAGAC GAAGAC(2/6)
511 GTCTTC
BsmAI 130 GAGAC GTCTC(1/5)
475 GAGAC
BsrI 329 ACTGG ACTGG(1/-1)
441 ACTGG
BstF5I 321 CATCC GGATG(2/0)
429 GGATG
BstUI 540 CGCG CG^CG
542 CGCG
EcoT14I 485 CCAAGG C^C[AT][AT]GG
527 CCAAGG
FokI 321 CATCC GGATG(9/13)
429 GGATG
HphI 245 TCACC GGTGA(8/7)
296 TCACC
MluNI 222 TGGCCA TGG^CCA
524 TGGCCA
MscI 222 TGGCCA TGG^CCA
524 TGGCCA
MvnI 540 CGCG CG^CG
542 CGCG
NlaIII 337 CATG CATG^
522 CATG
SfaNI 201 GATGC GCATC(5/9)
430 GATGC
StyI 485 CCAAGG C^C[AT][AT]GG
527 CCAAGG
TaqI 314 TCGA T^CGA
425 TCGA
TfiI 73 GATTC G^A[AT]TC
333 GATTC
TthHB8I 314 TCGA T^CGA
425 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BmyI BpmI
BsaJI BsgI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DdeI EaeI EcoRII Fnu4HI HaeIII
HhaI HinP1I HinfI ItaI MaeII
MboII MnlI MvaI MwoI PleI
Sau96I ScrFI TseI TspRI