Restriction map for
pFN21AE3208
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 911 CGCG CG^CG
ApaI 736 GGGCCC GGGCC^C
BalI 93 TGGCCA TGG^CCA
BanI 893 GGTGCC G^G[CT][GA]CC
BfaI 880 CTAG C^TAG
BsaAI 42 TACGTG [CT]AC^GT[GA]
BsmBI 622 GAGACG CGTCTC(1/5)
BspMI 218 ACCTGC ACCTGC(4/8)
BsrI 1057 ACTGG ACTGG(1/-1)
BssSI 307 CACGAG CACGAG(-5/-1)
BstUI 911 CGCG CG^CG
DpnII 468 GATC ^GATC
DraIII 388 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 242 CCAAGG C^C[AT][AT]GG
HaeII 949 GGCGCT [GA]GCGC^[CT]
HindIII 319 AAGCTT A^AGCTT
MaeI 880 CTAG C^TAG
MaeIII 686 GTCAC ^GT[ACGT]AC
MboI 468 GATC ^GATC
MluNI 93 TGGCCA TGG^CCA
MscI 93 TGGCCA TGG^CCA
MslI 348 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 911 CGCG CG^CG
NdeII 468 GATC ^GATC
PflMI 612 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 367 GAGTC GAGTC(4/5)
Sau3AI 468 GATC ^GATC
SexAI 584 ACCTGGT A^CC[AT]GGT
SmaI 371 CCCGGG CCC^GGG
SmlI 316 CTCAAG C^T[CT][GA]AG
StyI 242 CCAAGG C^C[AT][AT]GG
TfiI 483 GAATC G^A[AT]TC
Tsp45I 686 GTCAC ^GT[GC]AC
Van91I 612 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 879 TCTAGA T^CTAGA
XmaCI 371 CCCGGG C^CCGGG
XmaI 371 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AvaI 187 CCCGAG C^[CT]CG[GA]G
371 CCCGGG
BlpI 123 GCTGAGC GC^T[ACGT]AGC
509 GCTTAGC
Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC
509 GCTTAGC
BsmAI 622 GAGAC GTCTC(1/5)
637 GAGAC
BsmFI 204 GTCCC GGGAC(10/14)
369 GTCCC
BsoBI 187 CCCGAG C^[CT]CG[GA]G
371 CCCGGG
BsrBI 538 GAGCGG CCGCTC(-3/-3)
721 GAGCGG
BstAPI 378 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
387 GCACAGGGTGC
BstXI 50 CCATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
347 CCATCTGTGTGG
CelII 123 GCTGAGC GC^T[ACGT]AGC
509 GCTTAGC
CfoI 259 GCGC GCG^C
950 GCGC
DraII 735 GGGGCCC [GA]G^G[ACGT]CC[CT]
736 GGGCCCC
EaeI 93 TGGCCA [CT]^GGCC[GA]
248 TGGCCG
EcoNI 291 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1042 CCTCTGGGAGG
EcoO109I 735 GGGGCCC [GA]G^G[ACGT]CC[CT]
736 GGGCCCC
FauI 207 CCCGC CCCGC(4/6)
400 CCCGC
HhaI 259 GCGC GCG^C
950 GCGC
HinP1I 259 GCGC G^CGC
950 GCGC
HinfI 367 GAGTC G^A[ACGT]TC
483 GAATC
HphI 531 GGTGA GGTGA(8/7)
470 TCACC
MaeII 43 ACGT A^CGT
788 ACGT
MspA1I 121 CAGCTG C[AC]G^C[GT]G
712 CAGCTG
NlaIII 447 CATG CATG^
759 CATG
PvuII 121 CAGCTG CAG^CTG
712 CAGCTG
SapI 595 GAAGAGC GCTCTTC(1/4)
961 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI Bsp1286I BsrFI
BssKI BstF5I BstNI Cac8I Cfr10I
Cfr13I DdeI EarI EcoRII Fnu4HI
FokI HaeIII HapII HpaII ItaI
Ksp632I MboII MnlI MspI MvaI
MwoI NciI NlaIV PstI RsaI
Sau96I ScrFI SfaNI SfcI TseI
TspRI