Restriction map for
pFN21AE4088
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MaeI
MaeII MluI MluNI MroI MscI
MspA1I NarI NcoI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
TaqI TthHB8I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 176 CGCG CG^CG
AciI 177 GCGG CCGC(-3/-1)
AcsI 3 GAATTC [GA]^AATT[CT]
AfaI 117 GTAC GT^AC
AlwNI 495 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 817 GGGCCC GGGCC^C
ApoI 3 GAATTC [GA]^AATT[CT]
AspI 70 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 397 GCTGAGG CCTCAGC(-5/-2)
BclI 1275 TGATCA T^GATCA
BcnI 408 CCGGG CC^[GC]GG
BpmI 733 CTCCAG CTGGAG(16/14)
BsaBI 1270 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 665 CCAGGG C^C[ACGT][ACGT]GG
BsaWI 362 ACCGGA [AT]^CCGG[AT]
BsgI 812 GTGCAG GTGCAG(16/14)
BsmI 160 GAATGC GAATGC(1/-1)
BsrDI 225 CATTGC GCAATG(2/0)
BsrFI 104 GCCGGC [GA]^CCGG[CT]
BstAPI 139 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 176 CGCG CG^CG
BstXI 735 CCAGGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 693 GGATCT [GA]^GATC[CT]
Cfr10I 104 GCCGGC [GA]^CCGG[CT]
DraII 816 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 816 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 3 GAATTC G^AATTC
EcoRV 958 GATATC GAT^ATC
FbaI 1275 TGATCA T^GATCA
HindIII 449 AAGCTT A^AGCTT
MamI 1270 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1305 CAATTG C^AATTG
MflI 693 GGATCT [GA]^GATC[CT]
MunI 1305 CAATTG C^AATTG
MvnI 176 CGCG CG^CG
NaeI 104 GCCGGC GCC^GGC
NciI 408 CCGGG CC^[GC]GG
NdeI 1036 CATATG CA^TATG
NgoMIV 104 GCCGGC G^CCGGC
NlaIV 817 GGGCCC GG[ACGT]^[ACGT]CC
PflFI 70 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 117 GTAC GT^AC
SapI 577 GAAGAGC GCTCTTC(1/4)
SmlI 944 CTCAAG C^T[CT][GA]AG
SspI 1019 AATATT AAT^ATT
StuI 1330 AGGCCT AGG^CCT
Tth111I 70 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 693 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 952 GATCC GGATC(4/5)
693 GGATC
Asp700I 300 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
976 GAATGTTTTC
BbsI 168 GAAGAC GAAGAC(2/6)
22 GTCTTC
BpuAI 168 GAAGAC GAAGAC(2/6)
22 GTCTTC
EarI 577 GAAGAG CTCTTC(1/4)
1092 GAAGAG
Ksp632I 577 GAAGAG CTCTTC(1/4)
1092 GAAGAG
MslI 48 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
757 CACAGGTATG
PflMI 383 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
494 CCAGATGCTGG
PstI 871 CTGCAG CTGCA^G
1164 CTGCAG
SacI 415 GAGCTC GAGCT^C
805 GAGCTC
Van91I 383 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
494 CCAGATGCTGG
XmnI 300 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
976 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AluI AspHI BanII BbvI BmyI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI