Restriction map for
pFN21AE3202
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT22I FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 721 GTCTAC GT^[AC][GT]AC
AccII 835 CGCG CG^CG
AfaI 1162 GTAC GT^AC
AlwNI 1117 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1378 CCTGAGG CC^T[ACGT]AGG
BalI 860 TGGCCA TGG^CCA
BanI 1193 GGTGCC G^G[CT][GA]CC
BbsI 571 GAAGAC GAAGAC(2/6)
BbvCI 937 GCTGAGG CCTCAGC(-5/-2)
BciVI 1392 GGATAC GTATCC(6/5)
BclI 134 TGATCA T^GATCA
BfaI 1144 CTAG C^TAG
BglI 770 GCCGACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 571 GAAGAC GAAGAC(2/6)
BsaBI 1066 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1058 GAGACC GGTCTC(1/5)
BseRI 1003 GAGGAG GAGGAG(10/8)
BspHI 731 TCATGA T^CATGA
BstAPI 1257 GCACACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 835 CGCG CG^CG
Bsu36I 1378 CCTGAGG CC^T[ACGT]AGG
CfoI 323 GCGC GCG^C
DraIII 1258 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 860 TGGCCA [CT]^GGCC[GA]
EarI 918 GAAGAG CTCTTC(1/4)
Eco81I 1378 CCTGAGG CC^T[ACGT]AGG
EcoRI 274 GAATTC G^AATTC
EcoRV 191 GATATC GAT^ATC
FauI 991 GCGGG CCCGC(4/6)
FbaI 134 TGATCA T^GATCA
HgaI 1119 GCGTC GACGC(5/10)
HhaI 323 GCGC GCG^C
HinP1I 323 GCGC G^CGC
HincII 604 GTCAAC GT[CT]^[GA]AC
HindII 604 GTCAAC GT[CT]^[GA]AC
Ksp632I 918 GAAGAG CTCTTC(1/4)
MaeI 1144 CTAG C^TAG
MaeII 665 ACGT A^CGT
MamI 1066 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 860 TGGCCA TGG^CCA
MscI 860 TGGCCA TGG^CCA
MseI 227 TTAA T^TAA
MvnI 835 CGCG CG^CG
PaeR7I 155 CTCGAG C^TCGAG
PflMI 612 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 430 CTGCAG CTGCA^G
PvuII 935 CAGCTG CAG^CTG
RcaI 731 TCATGA T^CATGA
RsaI 1162 GTAC GT^AC
SacI 19 GAGCTC GAGCT^C
SexAI 31 ACCAGGT A^CC[AT]GGT
SfcI 430 CTGCAG C^T[GA][CT]AG
SmaI 1370 CCCGGG CCC^GGG
Tru9I 227 TTAA T^TAA
Van91I 612 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 155 CTCGAG C^TCGAG
XmaCI 1370 CCCGGG C^CCGGG
XmaI 1370 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 274 GAATTC [GA]^AATT[CT]
1045 GAATTT
ApoI 274 GAATTC [GA]^AATT[CT]
1045 GAATTT
AspHI 19 GAGCTC G[AT]GC[AT]^C
1264 GTGCTC
AvaI 155 CTCGAG C^[CT]CG[GA]G
1370 CCCGGG
AvaII 905 GGACC G^G[AT]CC
923 GGACC
BanII 19 GAGCTC G[GA]GC[CT]^C
93 GGGCTC
BglII 44 AGATCT A^GATCT
656 AGATCT
BpmI 5 CTCCAG CTGGAG(16/14)
1123 CTGGAG
BsgI 1255 CTGCAC GTGCAG(16/14)
694 GTGCAG
BsiHKAI 19 GAGCTC G[AT]GC[AT]^C
1264 GTGCTC
BsmAI 1058 GAGAC GTCTC(1/5)
3 GTCTC
BsoBI 155 CTCGAG C^[CT]CG[GA]G
1370 CCCGGG
Cac8I 74 GCAGGC GC[ACGT]^[ACGT]GC
839 GCAAGC
DraII 941 AGGCCCT [GA]G^G[ACGT]CC[CT]
971 AGGCCCT
EcoO109I 941 AGGCCCT [GA]G^G[ACGT]CC[CT]
971 AGGCCCT
EcoT14I 617 CCTTGG C^C[AT][AT]GG
1088 CCAAGG
MslI 126 CACAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1275 CACTCCTGTG
MspA1I 63 CAGCGG C[AC]G^C[GT]G
935 CAGCTG
SmlI 155 CTCGAG C^T[CT][GA]AG
1243 CTCAAG
StyI 617 CCTTGG C^C[AT][AT]GG
1088 CCAAGG
TfiI 51 GATTC G^A[AT]TC
1303 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI Asp700I BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI