Restriction map for
pFN21AB6106
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspHI AspI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BmyI BsaAI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT14I EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MluI MluNI MroI
MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 1289 GACGCC G[GA]^CG[CT]C
AhdI 1585 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1188 CCTGAGG CC^T[ACGT]AGG
AspEI 1585 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcnI 1178 CCCGG CC^[GC]GG
BpmI 847 CTCCAG CTGGAG(16/14)
BsaBI 1390 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1289 GACGCC G[GA]^CG[CT]C
BsaI 304 GAGACC GGTCTC(1/5)
BseRI 520 GAGGAG GAGGAG(10/8)
Bsp1407I 1454 TGTACA T^GTACA
BspHI 717 TCATGA T^CATGA
BsrGI 1454 TGTACA T^GTACA
BssSI 1474 CTCGTG CACGAG(-5/-1)
BstAPI 1350 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1322 GGATG GGATG(2/0)
Bsu36I 1188 CCTGAGG CC^T[ACGT]AGG
CfoI 1489 GCGC GCG^C
DraII 116 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 445 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1585 GACTCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1188 CCTGAGG CC^T[ACGT]AGG
EcoO109I 116 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 671 GAATTC G^AATTC
EcoRV 713 GATATC GAT^ATC
FauI 1596 CCCGC CCCGC(4/6)
FokI 1322 GGATG GGATG(9/13)
HapII 1179 CCGG C^CGG
HhaI 1489 GCGC GCG^C
Hin1I 1289 GACGCC G[GA]^CG[CT]C
HinP1I 1489 GCGC G^CGC
HpaII 1179 CCGG C^CGG
MamI 1390 GATTCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 200 CAATTG C^AATTG
MspI 1179 CCGG C^CGG
MunI 200 CAATTG C^AATTG
NciI 1178 CCCGG CC^[GC]GG
PaeR7I 1297 CTCGAG C^TCGAG
PpuMI 116 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1408 GACAACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 717 TCATGA T^CATGA
ScaI 131 AGTACT AGT^ACT
SmlI 1297 CTCGAG C^T[CT][GA]AG
SspBI 1454 TGTACA T^GTACA
SspI 791 AATATT AAT^ATT
XhoI 1297 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 492 CGCG CG^CG
1341 CGCG
AcsI 554 AAATTC [GA]^AATT[CT]
671 GAATTC
AlwNI 967 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1351 CAGATTCTG
ApoI 554 AAATTC [GA]^AATT[CT]
671 GAATTC
Asp700I 498 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1687 GAAGACATTC
AvaI 1297 CTCGAG C^[CT]CG[GA]G
1561 CCCGAG
BamHI 615 GGATCC G^GATCC
1040 GGATCC
BlpI 124 GCTAAGC GC^T[ACGT]AGC
1359 GCTCAGC
Bpu1102I 124 GCTAAGC GC^T[ACGT]AGC
1359 GCTCAGC
BsaJI 1169 CCCAGG C^C[ACGT][ACGT]GG
1430 CCCAGG
BsmFI 1593 GTCCC GGGAC(10/14)
1608 GTCCC
BsmI 148 GAATGC GAATGC(1/-1)
892 GAATGC
BsoBI 1297 CTCGAG C^[CT]CG[GA]G
1561 CCCGAG
BstUI 492 CGCG CG^CG
1341 CGCG
BstYI 615 GGATCC [GA]^GATC[CT]
1040 GGATCC
CelII 124 GCTAAGC GC^T[ACGT]AGC
1359 GCTCAGC
DraI 940 TTTAAA TTT^AAA
1108 TTTAAA
EarI 484 GAAGAG CTCTTC(1/4)
517 GAAGAG
Ksp632I 484 GAAGAG CTCTTC(1/4)
517 GAAGAG
MaeIII 869 GTTAC ^GT[ACGT]AC
950 GTTAC
MflI 615 GGATCC [GA]^GATC[CT]
1040 GGATCC
MvnI 492 CGCG CG^CG
1341 CGCG
SfaNI 904 GATGC GCATC(5/9)
1468 GATGC
TaqI 254 TCGA T^CGA
1298 TCGA
TthHB8I 254 TCGA T^CGA
1298 TCGA
XhoII 615 GGATCC [GA]^GATC[CT]
1040 GGATCC
XmnI 498 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1687 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbsI BbvI BfaI BpuAI BsiYI
BslI BsmAI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI HaeIII HgaI HinfI
ItaI MaeI MaeII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI TfiI
Tru9I TseI Tsp509I TspRI