Restriction map for
pFN21AE4151
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccII AccIII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BciVI
BclI BcnI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstEII BstPI
BstUI BstYI Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HapII HincII
HindII HindIII HpaI HpaII KasI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
PacI PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 803 GGTACC G^GTACC
AccI 1012 GTATAC GT^[AC][GT]AC
AclI 123 AACGTT AA^CGTT
AcyI 94 GGCGTC G[GA]^CG[CT]C
AlwNI 5 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1256 AGCGCT AGC^GCT
Asp700I 541 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 803 GGTACC G^GTACC
BanI 803 GGTACC G^G[CT][GA]CC
BbsI 36 GAAGAC GAAGAC(2/6)
BcgI 1051 CGAATGGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 36 GAAGAC GAAGAC(2/6)
BsaHI 94 GGCGTC G[GA]^CG[CT]C
BsgI 1568 GTGCAG GTGCAG(16/14)
BsmFI 1001 GGGAC GGGAC(10/14)
BsmI 772 GAATGC GAATGC(1/-1)
BspHI 98 TCATGA T^CATGA
BspMI 165 ACCTGC ACCTGC(4/8)
Bst1107I 1012 GTATAC GTA^TAC
BstAPI 1129 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1089 TTCGAA TT^CGAA
BstXI 1537 CCAATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1012 GTATAC GTA^TAC
CfoI 1257 GCGC GCG^C
DraII 1098 GGGTCCT [GA]G^G[ACGT]CC[CT]
Eco47III 1256 AGCGCT AGC^GCT
EcoO109I 1098 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 682 GATATC GAT^ATC
FauI 82 GCGGG CCCGC(4/6)
HaeII 1256 AGCGCT [GA]GCGC^[CT]
HaeIII 1288 GGCC GG^CC
HgaI 95 GCGTC GACGC(5/10)
HhaI 1257 GCGC GCG^C
Hin1I 94 GGCGTC G[GA]^CG[CT]C
HinP1I 1257 GCGC G^CGC
KpnI 803 GGTACC GGTAC^C
MspA1I 957 CCGCTG C[AC]G^C[GT]G
NheI 453 GCTAGC G^CTAGC
NspV 1089 TTCGAA TT^CGAA
PaeR7I 1112 CTCGAG C^TCGAG
PflMI 1266 CCATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1098 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 123 AACGTT AA^CGTT
RcaI 98 TCATGA T^CATGA
SapI 21 GAAGAGC GCTCTTC(1/4)
SfuI 1089 TTCGAA TT^CGAA
SspI 1496 AATATT AAT^ATT
TspRI 1559 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 1266 CCATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 465 TCTAGA T^CTAGA
XhoI 1112 CTCGAG C^TCGAG
XmnI 541 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 739 GTAC GT^AC
804 GTAC
AflII 726 CTTAAG C^TTAAG
778 CTTAAG
AvaI 59 CTCGGG C^[CT]CG[GA]G
1112 CTCGAG
BfrI 726 CTTAAG C^TTAAG
778 CTTAAG
BpmI 818 CTCCAG CTGGAG(16/14)
1160 CTGGAG
BsaI 1744 GAGACC GGTCTC(1/5)
1530 GGTCTC
BsaJI 1266 CCATGG C^C[ACGT][ACGT]GG
1543 CCATGG
BsoBI 59 CTCGGG C^[CT]CG[GA]G
1112 CTCGAG
DsaI 1266 CCATGG C^C[GA][CT]GG
1543 CCATGG
EcoT14I 1266 CCATGG C^C[AT][AT]GG
1543 CCATGG
HinfI 235 GAATC G^A[ACGT]TC
1416 GAATC
HphI 786 GGTGA GGTGA(8/7)
1534 TCACC
MaeII 124 ACGT A^CGT
134 ACGT
NcoI 1266 CCATGG C^CATGG
1543 CCATGG
NlaIV 803 GGTACC GG[ACGT]^[ACGT]CC
1098 GGGTCC
RsaI 739 GTAC GT^AC
804 GTAC
SphI 988 GCATGC GCATG^C
1244 GCATGC
StyI 1266 CCATGG C^C[AT][AT]GG
1543 CCATGG
TfiI 235 GAATC G^A[AT]TC
1416 GAATC
Tsp45I 131 GTGAC ^GT[GC]AC
1696 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BfaI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NspI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI Tru9I
TseI Tsp509I TthHB8I