Restriction map for
pFN21AE4263
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# NO CUT ENZYMES ( 74 enzymes) #
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Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BbrPI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsiWI Bsp1407I BspHI BsrDI BsrGI
BssHII Bst1107I BstAPI BstZ17I CelII
CpoI DraIII EcoNI EcoT22I FseI
FspI HindIII KasI KpnI KspI
MfeI MluI MunI NarI NdeI
NheI NruI NsiI PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI SspBI
SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 2554 GACGTC GACGT^C
AcyI 2554 GACGTC G[GA]^CG[CT]C
AflIII 1935 ACATGT A^C[GA][CT]GT
AhdI 193 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 215 GTGCAC G^TGCAC
AlwNI 1018 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 337 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2501 AGCGCT AGC^GCT
ApaI 1507 GGGCCC GGGCC^C
ApaLI 215 GTGCAC G^TGCAC
AspEI 193 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1577 TGGCCA TGG^CCA
BamHI 2732 GGATCC G^GATCC
BbvCI 963 CCTCAGC CCTCAGC(-5/-2)
BglI 46 GCCCGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1179 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2554 GACGTC G[GA]^CG[CT]C
BsmBI 2764 CGTCTC CGTCTC(1/5)
BspLU11I 1935 ACATGT A^CATGT
BssSI 56 CACGAG CACGAG(-5/-1)
BstEII 1980 GGTTACC G^GT[ACGT]ACC
BstPI 1980 GGTTACC G^GT[ACGT]ACC
Bsu36I 337 CCTAAGG CC^T[ACGT]AGG
DrdI 2548 GACGAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 193 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2501 AGCGCT AGC^GCT
Eco81I 337 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1980 GGTTACC G^GT[ACGT]ACC
EcoRI 1701 GAATTC G^AATTC
EcoRV 154 GATATC GAT^ATC
Hin1I 2554 GACGTC G[GA]^CG[CT]C
HpaI 636 GTTAAC GTT^AAC
MamI 1179 GATCCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1577 TGGCCA TGG^CCA
MscI 1577 TGGCCA TGG^CCA
NaeI 1817 GCCGGC GCC^GGC
NgoMIV 1817 GCCGGC G^CCGGC
NotI 3080 GCGGCCGC GC^GGCCGC
PaeR7I 21 CTCGAG C^TCGAG
PpuMI 371 AGGACCT [GA]G^G[AT]CC[CT]
SacI 1501 GAGCTC GAGCT^C
SbfI 456 CCTGCAGG CCTGCA^GG
Sse8387I 456 CCTGCAGG CCTGCA^GG
SspI 1324 AATATT AAT^ATT
StuI 1333 AGGCCT AGG^CCT
XhoI 21 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccII 1607 CGCG CG^CG
3154 CGCG
Asp700I 547 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2581 GAACTGATTC
AspI 656 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2535 GACGCTGTC
BclI 1139 TGATCA T^GATCA
2175 TGATCA
BcnI 3020 CCGGG CC^[GC]GG
3045 CCGGG
BfaI 703 CTAG C^TAG
1868 CTAG
BsgI 725 CTGCAC GTGCAG(16/14)
73 GTGCAG
BsiEI 751 CGGCCG CG[GA][CT]^CG
3081 CGGCCG
BsmI 1537 GAATGC GAATGC(1/-1)
2660 GAATGC
BspDI 37 ATCGAT AT^CGAT
884 ATCGAT
BsrFI 1817 GCCGGC [GA]^CCGG[CT]
2057 GCCGGT
BstBI 1970 TTCGAA TT^CGAA
2657 TTCGAA
BstUI 1607 CGCG CG^CG
3154 CGCG
BstXI 2063 CCAGGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2406 CCAACTGCTTGG
Cfr10I 1817 GCCGGC [GA]^CCGG[CT]
2057 GCCGGT
ClaI 37 ATCGAT AT^CGAT
884 ATCGAT
DraI 181 TTTAAA TTT^AAA
2805 TTTAAA
DraII 371 AGGACCT [GA]G^G[ACGT]CC[CT]
1507 GGGCCCC
EagI 751 CGGCCG C^GGCCG
3081 CGGCCG
EclXI 751 CGGCCG C^GGCCG
3081 CGGCCG
Eco52I 751 CGGCCG C^GGCCG
3081 CGGCCG
EcoO109I 371 AGGACCT [GA]G^G[ACGT]CC[CT]
1507 GGGCCCC
FbaI 1139 TGATCA T^GATCA
2175 TGATCA
MaeI 703 CTAG C^TAG
1868 CTAG
MvnI 1607 CGCG CG^CG
3154 CGCG
NciI 3020 CCGGG CC^[GC]GG
3045 CCGGG
NcoI 1976 CCATGG C^CATGG
2838 CCATGG
NspV 1970 TTCGAA TT^CGAA
2657 TTCGAA
PflFI 656 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2535 GACGCTGTC
SalI 140 GTCGAC G^TCGAC
1354 GTCGAC
SfuI 1970 TTCGAA TT^CGAA
2657 TTCGAA
Tth111I 656 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2535 GACGCTGTC
XcmI 1565 CCAAGCAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2984 CCATCCGCTGCGTGG
XmnI 547 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2581 GAACTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AccIII AciI AcsI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseAI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MroI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII