Restriction map for
pFN21AE3315
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI AspEI AspI
AvrII BbrPI BcgI BclI BfrI
BglII BlnI BsaAI BseAI BsiWI
BspDI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eam1105I Eco81I EcoO65I
EcoRI EcoT22I FbaI FseI HincII
HindII HpaI KspI MfeI MluI
MroI MunI NdeI NheI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI SphI Sse8387I SspI SwaI
Tth111I XcmI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 1591 AAATTT [GA]^AATT[CT]
Aor51HI 114 AGCGCT AGC^GCT
ApaI 259 GGGCCC GGGCC^C
ApoI 1591 AAATTT [GA]^AATT[CT]
BamHI 2235 GGATCC G^GATCC
BbvCI 580 CCTCAGC CCTCAGC(-5/-2)
BglI 1184 GCCTAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1673 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1673 GCTCAGC GC^T[ACGT]AGC
BsaWI 2351 ACCGGA [AT]^CCGG[AT]
Bsp1407I 609 TGTACA T^GTACA
BspLU11I 1751 ACATGT A^CATGT
BsrGI 609 TGTACA T^GTACA
CelII 1673 GCTCAGC GC^T[ACGT]AGC
DrdI 1021 GACATGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 114 AGCGCT AGC^GCT
EcoNI 687 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1175 GATATC GAT^ATC
HgaI 2443 GACGC GACGC(5/10)
HindIII 148 AAGCTT A^AGCTT
MseI 1316 TTAA T^TAA
NaeI 585 GCCGGC GCC^GGC
NcoI 1442 CCATGG C^CATGG
NgoMIV 585 GCCGGC G^CCGGC
NotI 99 GCGGCCGC GC^GGCCGC
NspI 1751 ACATGT [GA]CATG^[CT]
PaeR7I 1466 CTCGAG C^TCGAG
SacI 289 GAGCTC GAGCT^C
SapI 1657 GAAGAGC GCTCTTC(1/4)
ScaI 1206 AGTACT AGT^ACT
SexAI 1271 ACCTGGT A^CC[AT]GGT
SmaI 15 CCCGGG CCC^GGG
SpeI 1162 ACTAGT A^CTAGT
SspBI 609 TGTACA T^GTACA
TfiI 1238 GATTC G^A[AT]TC
Tru9I 1316 TTAA T^TAA
Tsp509I 1592 AATT ^AATT
XbaI 1117 TCTAGA T^CTAGA
XhoI 1466 CTCGAG C^TCGAG
XmaCI 15 CCCGGG C^CCGGG
XmaI 15 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 226 GGCGCC G[GA]^CG[CT]C
301 GGCGCC
AflIII 770 ACGTGT A^C[GA][CT]GT
1751 ACATGT
Asp700I 1326 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2331 GAACAACTTC
AviII 311 TGCGCA TGC^GCA
2261 TGCGCA
BalI 339 TGGCCA TGG^CCA
2549 TGGCCA
BbeI 226 GGCGCC GGCGC^C
301 GGCGCC
BbsI 1492 GTCTTC GAAGAC(2/6)
2457 GTCTTC
BciVI 894 GGATAC GTATCC(6/5)
1680 GGATAC
BpuAI 1492 GTCTTC GAAGAC(2/6)
2457 GTCTTC
BsaBI 1050 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2403 GATCTACATC
BsaHI 226 GGCGCC G[GA]^CG[CT]C
301 GGCGCC
BsiEI 100 CGGCCG CG[GA][CT]^CG
517 CGGCCG
BsmBI 1090 CGTCTC CGTCTC(1/5)
1972 GAGACG
BsmI 1140 GAATGC GAATGC(1/-1)
1485 GAATGC
BstXI 1617 CCAAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1722 CCAGGGCAATGG
EagI 100 CGGCCG C^GGCCG
517 CGGCCG
EarI 2479 CTCTTC CTCTTC(1/4)
1657 GAAGAG
EclXI 100 CGGCCG C^GGCCG
517 CGGCCG
Eco52I 100 CGGCCG C^GGCCG
517 CGGCCG
FspI 311 TGCGCA TGC^GCA
2261 TGCGCA
Hin1I 226 GGCGCC G[GA]^CG[CT]C
301 GGCGCC
KasI 226 GGCGCC G^GCGCC
301 GGCGCC
Ksp632I 2479 CTCTTC CTCTTC(1/4)
1657 GAAGAG
MamI 1050 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2403 GATCTACATC
MluNI 339 TGGCCA TGG^CCA
2549 TGGCCA
MscI 339 TGGCCA TGG^CCA
2549 TGGCCA
MslI 2064 CATCGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2559 CACTGACATG
NarI 226 GGCGCC GG^CGCC
301 GGCGCC
PflMI 1290 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2028 CCAACAGGTGG
PstI 403 CTGCAG CTGCA^G
1231 CTGCAG
SfoI 226 GGCGCC GGC^GCC
301 GGCGCC
SmlI 1466 CTCGAG C^T[CT][GA]AG
1516 CTCAAG
StuI 1359 AGGCCT AGG^CCT
1512 AGGCCT
Van91I 1290 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2028 CCAACAGGTGG
XmnI 1326 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2331 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
AlwI AlwNI Asp718I AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI KpnI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII