Restriction map for
pFN21AE4172
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BclI BcnI BfrI BlnI BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
FauI FbaI FseI FspI HgaI
Hin1I HpaI KasI KpnI Ksp632I
KspI MluI MluNI MroI MscI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1031 GTCTAC GT^[AC][GT]AC
AlwNI 73 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 111 GGGCCC GGGCC^C
AspHI 1749 GAGCAC G[AT]GC[AT]^C
BanI 62 GGCACC G^G[CT][GA]CC
BanII 111 GGGCCC G[GA]GC[CT]^C
BbvCI 129 GCTGAGG CCTCAGC(-5/-2)
BciVI 335 GGATAC GTATCC(6/5)
BglI 113 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 54 GCTCAGC GC^T[ACGT]AGC
BpmI 1016 CTCCAG CTGGAG(16/14)
Bpu1102I 54 GCTCAGC GC^T[ACGT]AGC
BsaBI 1726 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 588 GTGCAG GTGCAG(16/14)
BsiHKAI 1749 GAGCAC G[AT]GC[AT]^C
BsmFI 1571 GTCCC GGGAC(10/14)
BsmI 751 GAATGC GAATGC(1/-1)
BspLU11I 384 ACATGT A^CATGT
BspMI 491 GCAGGT ACCTGC(4/8)
BsrDI 1579 GCAATG GCAATG(2/0)
BstAPI 1136 GCAATTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1628 GGATG GGATG(2/0)
BstXI 1574 CCACTGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 54 GCTCAGC GC^T[ACGT]AGC
CfoI 76 GCGC GCG^C
DraII 111 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 111 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 628 GAATTC G^AATTC
EcoRV 433 GATATC GAT^ATC
FokI 1628 GGATG GGATG(9/13)
HaeII 75 GGCGCT [GA]GCGC^[CT]
HhaI 76 GCGC GCG^C
HinP1I 76 GCGC G^CGC
HincII 1061 GTCAAC GT[CT]^[GA]AC
HindII 1061 GTCAAC GT[CT]^[GA]AC
HphI 482 TCACC GGTGA(8/7)
MamI 1726 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 57 CAGCTG C[AC]G^C[GT]G
NspI 384 ACATGT [GA]CATG^[CT]
PleI 1671 GACTC GAGTC(4/5)
PstI 656 CTGCAG CTGCA^G
PvuII 57 CAGCTG CAG^CTG
ScaI 1861 AGTACT AGT^ACT
SfcI 656 CTGCAG C^T[GA][CT]AG
SmlI 42 CTTGAG C^T[CT][GA]AG
SspI 1561 AATATT AAT^ATT
StuI 20 AGGCCT AGG^CCT
TaqI 342 TCGA T^CGA
TthHB8I 342 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflIII 384 ACATGT A^C[GA][CT]GT
809 ACACGT
Asp700I 252 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1885 GAAGGAGTTC
BbsI 35 GAAGAC GAAGAC(2/6)
1668 GAAGAC
BglII 533 AGATCT A^GATCT
1266 AGATCT
BmyI 111 GGGCCC G[AGT]GC[ACT]^C
1749 GAGCAC
BpuAI 35 GAAGAC GAAGAC(2/6)
1668 GAAGAC
BseRI 136 GAGGAG GAGGAG(10/8)
139 GAGGAG
Bsp1286I 111 GGGCCC G[AGT]GC[ACT]^C
1749 GAGCAC
BstYI 533 AGATCT [GA]^GATC[CT]
1266 AGATCT
Cac8I 59 GCTGGC GC[ACGT]^[ACGT]GC
1011 GCTTGC
Cfr13I 111 GGGCC G^G[ACGT]CC
112 GGCCC
EcoNI 23 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
485 CCTTTTGCAGG
EcoT14I 268 CCAAGG C^C[AT][AT]GG
687 CCTTGG
HapII 145 CCGG C^CGG
1626 CCGG
HindIII 262 AAGCTT A^AGCTT
472 AAGCTT
HpaII 145 CCGG C^CGG
1626 CCGG
MaeII 185 ACGT A^CGT
811 ACGT
MflI 533 AGATCT [GA]^GATC[CT]
1266 AGATCT
MspI 145 CCGG C^CGG
1626 CCGG
Sau96I 111 GGGCC G^G[ACGT]CC
112 GGCCC
SpeI 648 ACTAGT A^CTAGT
1615 ACTAGT
StyI 268 CCAAGG C^C[AT][AT]GG
687 CCTTGG
XhoII 533 AGATCT [GA]^GATC[CT]
1266 AGATCT
XmnI 252 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1885 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstNI DdeI DpnII
EcoRII EcoT22I Fnu4HI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MfeI MnlI MseI MslI MunI
MvaI MwoI NdeII NlaIII NlaIV
NsiI RsaI Sau3AI ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI