Restriction map for
pFN21AE4055
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII AccIII AcyI AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BcnI BglI
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiWI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoT22I FauI FseI FspI HaeII
HgaI Hin1I KasI KspI MfeI
MluI MluNI MroI MscI MunI
NarI NciI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PpuMI PshBI PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 265 GGTACC G^GTACC
AflII 811 CTTAAG C^TTAAG
AlwNI 884 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 144 CCTAAGG CC^T[ACGT]AGG
Asp718I 265 GGTACC G^GTACC
AspI 1303 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1118 CTCGAG C^[CT]CG[GA]G
AvaII 544 GGTCC G^G[AT]CC
BanI 265 GGTACC G^G[CT][GA]CC
BciVI 139 GGATAC GTATCC(6/5)
BfrI 811 CTTAAG C^TTAAG
BglII 1821 AGATCT A^GATCT
BpmI 1664 CTCCAG CTGGAG(16/14)
BsaAI 1378 TACGTG [CT]AC^GT[GA]
BsiEI 1280 CGACCG CG[GA][CT]^CG
BsmBI 2470 CGTCTC CGTCTC(1/5)
BsoBI 1118 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2010 TGTACA T^GTACA
BspHI 836 TCATGA T^CATGA
BspMI 2112 GCAGGT ACCTGC(4/8)
BsrFI 2033 GCCGGC [GA]^CCGG[CT]
BsrGI 2010 TGTACA T^GTACA
BstAPI 637 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2189 CCACCCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 144 CCTAAGG CC^T[ACGT]AGG
CfoI 2215 GCGC GCG^C
Cfr10I 2033 GCCGGC [GA]^CCGG[CT]
DraIII 1193 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2343 GACTTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2507 CCATGG C^C[GA][CT]GG
EaeI 2466 TGGCCG [CT]^GGCC[GA]
Eco81I 144 CCTAAGG CC^T[ACGT]AGG
EcoRV 1793 GATATC GAT^ATC
HapII 2034 CCGG C^CGG
HhaI 2215 GCGC GCG^C
HinP1I 2215 GCGC G^CGC
HpaI 1183 GTTAAC GTT^AAC
HpaII 2034 CCGG C^CGG
KpnI 265 GGTACC GGTAC^C
MspA1I 2392 CAGCTG C[AC]G^C[GT]G
MspI 2034 CCGG C^CGG
NaeI 2033 GCCGGC GCC^GGC
NcoI 2507 CCATGG C^CATGG
NdeI 741 CATATG CA^TATG
NgoMIV 2033 GCCGGC G^CCGGC
PaeR7I 1118 CTCGAG C^TCGAG
PflFI 1303 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2505 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2392 CAGCTG CAG^CTG
RcaI 836 TCATGA T^CATGA
SacI 185 GAGCTC GAGCT^C
ScaI 231 AGTACT AGT^ACT
SpeI 696 ACTAGT A^CTAGT
SspBI 2010 TGTACA T^GTACA
StuI 2626 AGGCCT AGG^CCT
Tth111I 1303 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 462 CCAGTCTATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1118 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 1559 CGCG CG^CG
2214 CGCG
AclI 1108 AACGTT AA^CGTT
1234 AACGTT
AspHI 185 GAGCTC G[AT]GC[AT]^C
1754 GTGCTC
BanII 185 GAGCTC G[GA]GC[CT]^C
855 GAGCCC
BsaBI 1537 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1711 GATGATGATC
BsiHKAI 185 GAGCTC G[AT]GC[AT]^C
1754 GTGCTC
BsmFI 1078 GGGAC GGGAC(10/14)
1309 GTCCC
BsmI 1553 GCATTC GAATGC(1/-1)
1799 GCATTC
BstUI 1559 CGCG CG^CG
2214 CGCG
DraI 10 TTTAAA TTT^AAA
1020 TTTAAA
HincII 88 GTTGAC GT[CT]^[GA]AC
1183 GTTAAC
HindII 88 GTTGAC GT[CT]^[GA]AC
1183 GTTAAC
MamI 1537 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1711 GATGATGATC
MslI 956 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2508 CATGGTCATG
MvnI 1559 CGCG CG^CG
2214 CGCG
NspI 929 ACATGC [GA]CATG^[CT]
1897 GCATGT
PflMI 462 CCAGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
597 CCAACAAGTGG
Psp1406I 1108 AACGTT AA^CGTT
1234 AACGTT
PstI 1336 CTGCAG CTGCA^G
2612 CTGCAG
Van91I 462 CCAGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
597 CCAACAAGTGG
XbaI 207 TCTAGA T^CTAGA
298 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI BbsI BbvI BclI
BfaI BmyI BpuAI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII