Restriction map for
pFN21AE4244
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV FauI FseI FspI HapII
Hin1I HpaII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 638 GTAGAC GT^[AC][GT]AC
Alw44I 299 GTGCAC G^TGCAC
AocI 1003 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1187 AGCGCT AGC^GCT
ApaLI 299 GTGCAC G^TGCAC
BciVI 2290 GTATCC GTATCC(6/5)
BclI 1845 TGATCA T^GATCA
BsaAI 388 CACGTA [CT]AC^GT[GA]
BsiYI 1003 CCTGAGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1003 CCTGAGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 693 GTCCC GGGAC(10/14)
BsmI 2067 GCATTC GAATGC(1/-1)
Bsp1407I 449 TGTACA T^GTACA
BspMI 2595 ACCTGC ACCTGC(4/8)
BsrGI 449 TGTACA T^GTACA
BssSI 2546 CACGAG CACGAG(-5/-1)
BstBI 2060 TTCGAA TT^CGAA
BstF5I 1333 CATCC GGATG(2/0)
Bsu36I 1003 CCTGAGG CC^T[ACGT]AGG
Cac8I 1294 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 1188 GCGC GCG^C
Eco47III 1187 AGCGCT AGC^GCT
Eco81I 1003 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1962 CCTTGG C^C[AT][AT]GG
EcoT22I 2053 ATGCAT ATGCA^T
FbaI 1845 TGATCA T^GATCA
FokI 1333 CATCC GGATG(9/13)
HaeII 1187 AGCGCT [GA]GCGC^[CT]
HaeIII 2674 GGCC GG^CC
HgaI 2475 GCGTC GACGC(5/10)
HhaI 1188 GCGC GCG^C
HinP1I 1188 GCGC G^CGC
HindIII 184 AAGCTT A^AGCTT
HpaI 1041 GTTAAC GTT^AAC
MspA1I 2552 CAGCGG C[AC]G^C[GT]G
NdeI 1441 CATATG CA^TATG
NsiI 2053 ATGCAT ATGCA^T
NspI 1317 GCATGT [GA]CATG^[CT]
NspV 2060 TTCGAA TT^CGAA
PstI 116 CTGCAG CTGCA^G
SapI 139 GCTCTTC GCTCTTC(1/4)
SexAI 2141 ACCTGGT A^CC[AT]GGT
SfuI 2060 TTCGAA TT^CGAA
SspBI 449 TGTACA T^GTACA
StyI 1962 CCTTGG C^C[AT][AT]GG
SwaI 227 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 299 GTGCAC G[AT]GC[AT]^C
1261 GAGCAC
BbvI 2551 GCAGC GCAGC(8/12)
481 GCTGC
BcgI 528 CGAAAATGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1743 GCAAAGTTTTCG
BsiHKAI 299 GTGCAC G[AT]GC[AT]^C
1261 GAGCAC
BstYI 38 AGATCC [GA]^GATC[CT]
2637 AGATCC
EarI 140 CTCTTC CTCTTC(1/4)
6 GAAGAG
Fnu4HI 481 GCTGC GC^[ACGT]GC
2551 GCAGC
HincII 1041 GTTAAC GT[CT]^[GA]AC
2532 GTCAAC
HindII 1041 GTTAAC GT[CT]^[GA]AC
2532 GTCAAC
ItaI 481 GCTGC GC^[ACGT]GC
2551 GCAGC
Ksp632I 140 CTCTTC CTCTTC(1/4)
6 GAAGAG
MaeII 389 ACGT A^CGT
1602 ACGT
MflI 38 AGATCC [GA]^GATC[CT]
2637 AGATCC
MwoI 2554 GCGGATTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2675 GCCTTGATAGC
SmlI 31 CTTGAG C^T[CT][GA]AG
1457 CTCAAG
TseI 481 GCTGC G^C[AT]GC
2551 GCAGC
XhoII 38 AGATCC [GA]^GATC[CT]
2637 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbsI BfaI
BmyI BpmI BpuAI BsaJI BseRI
Bsp1286I BspHI BsrI BssKI BstNI
Cfr13I DdeI DpnII DraI DraII
EcoO109I EcoRII HinfI HphI MaeI
MaeIII MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
PpuMI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SspI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XmnI