################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AccIII AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI AspI AviII AvrII BbeI BciVI BclI BfrI BlnI BsaWI BseAI BsiWI Bsp1407I BspEI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CpoI DraI DraIII DrdI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1617 GACGTC GACGT^C Acc65I 1730 GGTACC G^GTACC AccI 1279 GTCTAC GT^[AC][GT]AC AcyI 1617 GACGTC G[GA]^CG[CT]C AflIII 1784 ACATGT A^C[GA][CT]GT AocI 2191 CCTGAGG CC^T[ACGT]AGG ApaI 2286 GGGCCC GGGCC^C Asp718I 1730 GGTACC G^GTACC BalI 248 TGGCCA TGG^CCA BbrPI 2133 CACGTG CAC^GTG BglI 1637 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1457 AGATCT A^GATCT BlpI 1632 GCTGAGC GC^T[ACGT]AGC BpmI 654 CTGGAG CTGGAG(16/14) Bpu1102I 1632 GCTGAGC GC^T[ACGT]AGC BsaAI 2133 CACGTG [CT]AC^GT[GA] BsaBI 1458 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1617 GACGTC G[GA]^CG[CT]C BsmI 441 GCATTC GAATGC(1/-1) BspDI 1855 ATCGAT AT^CGAT BspHI 2021 TCATGA T^CATGA BspLU11I 1784 ACATGT A^CATGT BstAPI 1182 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1719 GGTGACC G^GT[ACGT]ACC BstPI 1719 GGTGACC G^GT[ACGT]ACC BstXI 1772 CCACGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2191 CCTGAGG CC^T[ACGT]AGG CelII 1632 GCTGAGC GC^T[ACGT]AGC ClaI 1855 ATCGAT AT^CGAT Eco81I 2191 CCTGAGG CC^T[ACGT]AGG EcoO65I 1719 GGTGACC G^GT[ACGT]ACC EcoT14I 1760 CCAAGG C^C[AT][AT]GG HgaI 1226 GCGTC GACGC(5/10) Hin1I 1617 GACGTC G[GA]^CG[CT]C KpnI 1730 GGTACC GGTAC^C MamI 1458 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 248 TGGCCA TGG^CCA MscI 248 TGGCCA TGG^CCA MseI 103 TTAA T^TAA PmaCI 2133 CACGTG CAC^GTG PmlI 2133 CACGTG CAC^GTG PstI 1210 CTGCAG CTGCA^G RcaI 2021 TCATGA T^CATGA SbfI 1209 CCTGCAGG CCTGCA^GG SphI 330 GCATGC GCATG^C Sse8387I 1209 CCTGCAGG CCTGCA^GG StyI 1760 CCAAGG C^C[AT][AT]GG Tru9I 103 TTAA T^TAA XcmI 1557 CCAGCTGCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 44 AAATTC [GA]^AATT[CT] 108 AAATTC AhdI 745 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1783 GACATGTGGTC Aor51HI 1982 AGCGCT AGC^GCT 2062 AGCGCT ApoI 44 AAATTC [GA]^AATT[CT] 108 AAATTC AspEI 745 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1783 GACATGTGGTC BamHI 1595 GGATCC G^GATCC 1687 GGATCC BbvCI 1371 GCTGAGG CCTCAGC(-5/-2) 2065 GCTGAGG BfaI 335 CTAG C^TAG 1895 CTAG BsmBI 701 GAGACG CGTCTC(1/5) 2239 GAGACG Eam1105I 745 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1783 GACATGTGGTC Eco47III 1982 AGCGCT AGC^GCT 2062 AGCGCT KspI 679 CCGCGG CCGC^GG 2282 CCGCGG MaeI 335 CTAG C^TAG 1895 CTAG MslI 1464 CATCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1773 CACGGCCGTG NaeI 1203 GCCGGC GCC^GGC 1642 GCCGGC NgoMIV 1203 GCCGGC G^CCGGC 1642 GCCGGC NspI 330 GCATGC [GA]CATG^[CT] 1784 ACATGT PflMI 647 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1026 CCACCTCTTGG PpuMI 743 GGGACCT [GA]G^G[AT]CC[CT] 1888 GGGACCC PvuII 1558 CAGCTG CAG^CTG 2215 CAGCTG SacI 1174 GAGCTC GAGCT^C 1924 GAGCTC SacII 679 CCGCGG CCGC^GG 2282 CCGCGG SmaI 1840 CCCGGG CCC^GGG 2047 CCCGGG StuI 1700 AGGCCT AGG^CCT 2199 AGGCCT Van91I 647 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1026 CCACCTCTTGG XmaCI 1840 CCCGGG C^CCGGG 2047 CCCGGG XmaI 1840 CCCGGG C^CCGGG 2047 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BmyI BpuAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI